Article (Scientific journals)
Developmental regulation of the composite CAG promoter activity in the murine T lymphocyte cell lineage.
Baup, D.; Moser, M.; Schurmans, Stéphane et al.
2009In Genesis, 47, p. 799-804
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Keywords :
transgenesis; CAG promoter; T cell development; thymus
Abstract :
[en] Promoter selection is of utmost importance for the study of in vivo gene function using transgenic models. In the present study, we have analyzed the expression of the GFP marker under the control of the composite CAG promoter in the lymphoid compartment of several transgenic mouse strains. Despite the ability of the CAG promoter to drive gene expression in almost all tissues examined to date, its activity appears to be developmentally regulated within the T lymphocyte cell lineage. In particular, CD4 and CD8-expressing, thymic immature T cells displayed lower levels of the GFP marker when compared with both bone marrow precursors and mature circulating T cells, suggesting a transient downregulation of CAG activity during T cell development. Alternative promoters may therefore be preferred for the study of T cell development in vivo using a transgenic approach.
Disciplines :
Genetics & genetic processes
Biochemistry, biophysics & molecular biology
Author, co-author :
Baup, D.;  Université Libre de Bruxelles - ULB > Laboratoire de Physiologie Animale
Moser, M.;  Université Libre de Bruxelles - ULB > Laboratoire de Physiologie Animale
Schurmans, Stéphane  ;  Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire
Leo, O.;  Université Libre de Bruxelles - ULB > Laboratoire de Physiologie Animale
Language :
English
Title :
Developmental regulation of the composite CAG promoter activity in the murine T lymphocyte cell lineage.
Publication date :
2009
Journal title :
Genesis
ISSN :
1526-954X
eISSN :
1526-968X
Publisher :
Wiley Liss, Inc., New York, United States - New York
Volume :
47
Pages :
799-804
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 November 2009

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