Neospora caninum; direct ELISA; IFAT; C-ELISA; dog; seroprevalence
Abstract :
[en] An enzyme-linked immunosorbent assay (ELISA) was developed in our laboratory and used to determine the seroprevalence of Neospora caninum in three different dog populations in Belgium: healthy dogs from cattle farms and urban dogs with or without various neurological disorders. The test was validated and compared with two other tests: an indirect fluorescent antibody test (IFAT) and a commercially available competitive enzyme-linked immunosorbent assay (C-ELISA). The study showed a good correlation between the IFAT and the ELISA developed. When the two tests were compared with the C-ELISA, moderate positive and negative agreement indices were observed. Using our ELISA and the IFAT techniques, a high prevalence was found in farm dogs. This result showed that the neurological symptoms are not usually associated with the Neospora infection. In conclusion, the ELISA developed in our laboratory could replace the IFAT for the screening of a large number of dogs' sera. (C) 2004 Elsevier B.V. All rights reserved.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...) Veterinary medicine & animal health Entomology & pest control
Author, co-author :
Lasri, S.
De Meerschman, F.
Rettigner, C.
Focant, Charles ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Comparison of three techniques for the serological diagnosis of Neospora caninum in the dog and their use for epidemiological studies
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