Reference : Comparison of three techniques for the serological diagnosis of Neospora caninum in t...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Life sciences : Veterinary medicine & animal health
Life sciences : Entomology & pest control
Comparison of three techniques for the serological diagnosis of Neospora caninum in the dog and their use for epidemiological studies
Lasri, S. [> > > >]
De Meerschman, F. [> > > >]
Rettigner, C. [> > > >]
Focant, Charles mailto [Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire >]
Losson, Bertrand mailto [Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Veterinary Parasitology
Elsevier Science Bv
Yes (verified by ORBi)
[en] Neospora caninum ; direct ELISA ; IFAT ; C-ELISA ; dog ; seroprevalence
[en] An enzyme-linked immunosorbent assay (ELISA) was developed in our laboratory and used to determine the seroprevalence of Neospora caninum in three different dog populations in Belgium: healthy dogs from cattle farms and urban dogs with or without various neurological disorders. The test was validated and compared with two other tests: an indirect fluorescent antibody test (IFAT) and a commercially available competitive enzyme-linked immunosorbent assay (C-ELISA). The study showed a good correlation between the IFAT and the ELISA developed. When the two tests were compared with the C-ELISA, moderate positive and negative agreement indices were observed. Using our ELISA and the IFAT techniques, a high prevalence was found in farm dogs. This result showed that the neurological symptoms are not usually associated with the Neospora infection. In conclusion, the ELISA developed in our laboratory could replace the IFAT for the screening of a large number of dogs' sera. (C) 2004 Elsevier B.V. All rights reserved.

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