Damage and functional recovery of the Atlantic cod (Gadus morhua) inner ear hair cells following local injection of gentamicin

Faucher, Karine; Aas-Hansen, Øyvind; Damsgård, Borge; Laukli, Einar; Stenklev, Niels-Christian

doi:10.1080/14992020902738029

Article (Scientific journals)

Damage and functional recovery of the Atlantic cod (Gadus morhua) inner ear hair cells following local injection of gentamicin

Faucher, Karine; Aas-Hansen, Øyvind; Damsgård, Borge et al.

2009 • In International Journal of Audiology, 48 (7), p. 456-464

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/27524

DOI
10.1080/14992020902738029

PubMed
19925332

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Keywords :

Fish hearing; AEP; SEM; Gentamicin; Inner ear hair cells; Regeneration

Abstract :

[en] This study addresses the ultrastructural and functional damage and subsequent recovery of the inner ear in the Atlantic cod following intrasaccular gentamicin injection. Inner ear damage was assessed using SEM and measurements of AEP following 250-Hz pure-tone stimuli. Data from gentamicin-treated fish were compared with control (no injection) and sham (injection of saline) fish. Control fish had normal response thresholds associated with welldeveloped hair cell bundles in their macula sacculi. Sham fish had higher response thresholds compared with control fish during the first week post-intervention, but response thresholds were subsequently normal. Treated fish displayed significant inner ear damage associated with an increased average AEP threshold on the third day following treatment. Thereafter, inner ear tissue displayed signs of progressive regeneration until it was comparable to controls from the 14th day. Response thresholds were similar to those of control fish from the 17th day following treatment. These observations suggest that the macula sacculi of Atlantic cod can regenerate towards a near-complete functional and ultrastructural recovery within 17-21 days following ototoxic gentamicin treatment.

Disciplines :

Aquatic sciences & oceanology

Author, co-author :

Faucher, Karine ; Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Morphologie fonctionnelle et évolutive

Aas-Hansen, Øyvind

Damsgård, Borge

Laukli, Einar

Stenklev, Niels-Christian

Language :

English

Title :

Damage and functional recovery of the Atlantic cod (Gadus morhua) inner ear hair cells following local injection of gentamicin

Publication date :

2009

Journal title :

International Journal of Audiology

ISSN :

1499-2027

eISSN :

1708-8186

Publisher :

Taylor & Francis, Abingdon, United Kingdom

Volume :

Issue :

Pages :

456-464

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 04 November 2009

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Bibliography

Avallone, B., Fascio, U., Balsao, G. & Marmo, F. 2008. Gentamicin ototoxicity in the saccule of the lizard Podarcis Sicula induces hair cell recovery and regeneration. Hear Res, 235, 15-22.
Chapman, C.J. & Hawkins, A.D. 1973. A field study of hearing in the cod. Gadus morhua L J Comp Physiol A, 85, 147-167.
Corey, D.P. & Hudspeth, A.J. 1983. Analysis of the microphonic potential of the bullfrog's sacculus. J Neurosci, 3, 942-961.
Corwin, J.T., Bullock, T.H. & Schweitzer, J. 1982. The auditory brain stem response in five vertebrate classes. Electroencephalogr Clin Neurophysiol, 54, 629-641.
Dale, T. 1976. The labyrinthine mechanoreceptor organs of the cod Gadus morhua L. (Teleostei: Gadidae). A scanning electron microscopical study, with special reference to the morphological polarization of the macular sensory cells. Norway Journal of Zoology, 24, 85-128.
Dulon, D., Aurousseau, C., Erre, J.P. & Aran, J.M. 1988. Relationship between the nephrotoxicity and ototoxicity induced by gentamicin in the guinea pig. Acta Otolaryngol (Stockh), 106, 219-225.
Enger, P.S. & Andersen, R. 1967. An electrophysiological field study of hearing in fish. Comp Biochem Physiol, 22, 517-525.
Faucher, K., Aas-Hansen, O., Damsgard, B. & Stenklev, N.C. 2008. Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations. Hear Res, 240, 12-21.
Gale, J.E., Meyers, J.R., Periasamy, A. & Corwin, J.T. 2002. Survival of bundleless hair cells and subsequent bundle replacement in the bullfrog's saccule. J Neurobiol, 50, 81-92.
Harris, J.A., Cheng, A.G., Cunningham, L.L., Macdonald, G., Raible, D.W., et al. 2003. Neomycin-induced hair cell death and rapid regeneration in the lateral line of zebrafish (Danio rerio). JARO, 04, 219-234.
Hawkins, A.D. 1972a. The role played by the swimbladder in the hearing in fish. J Physiol, 227, 47P.
Hawkins, A.D. 1972b. The role played by the swimbladder in the hearing of cod. J Physiol, 227, 10P.
Hecox, K., Galambos, R. 1974. Brain stem auditory evoked responses in human infants and adults. Arch Otolaryngol, 99, 30-33.
Hudspeth, A.J. 1982. Extracellular current flow and the site of transduction by vertebrate hair cells. J Neurosci, 2, 1-10.
Hudspeth, A.J. & Kroese, A.B.A. 1983. Voltage-dependent interaction of dihydrostreptomycin with the transduction channels in bullfrog sensory hair cells. J Physiol, 345, 66.
Kaus, S. 1987. The effect of aminoglycoside antibiotics on the lateral line organ of Aplocheilus lineatus (Cyprinodontidae). Acta Otolaryngol, (Stockh), 103, 291-298.
Kenyon, N., Ladich, F. & Yan, H.Y. 1998. A comparative study of hearing ability in fishes: The auditory brainstem response approach. J Comp Physiol A, 182, 307-318.
Kroese, B.A., Das, A. & Hudspeth, A.J. 1989. Blockage of the transduction channels of hair cells in the bullfrog's sacculus by aminoglycoside antibiotics. Hear Res, 37, 203-217.
Lombarte, A., Yan, H.Y., Popper, A.N., Chang, J.S. & Platt, C. 1993. Damage and regeneration of hair cell ciliary bundles in a fish ear following treatment with gentamicin. Hear Res, 64, 166-174.
Matsuura, S., Ikeda, K. & Furukawa, T. 1971. Effects of streptomycin, kanamycin, quinine, and other drugs on the microphonic potentials of goldfish sacculus. Jap J Physiol, 21, 579-590.
Nakagawa, T., Kim, T.S., Murai, N., Endo, T., Iguchi, F., et al. 2003. A novel technique for inducing local inner ear damage. Hear Res, 176, 122-127.
Presson, J.C. & Popper, A.N. 1990. Possible precursors to new hair cells, support cells, and Schwann cells in the ear of a post-embryonic fish. Hear Res, 46, 9-21.
Rouse, G.W. & Pickles, J.O. 1991. Paired development of hair cells in neuromasts of the teleost lateral line. Proc R Soc Lond B, 246, 123-128.
Sand, O. & Enger, P.S. 1973. Evidence for an auditory function of the swimbladder in the Cod. J Exp Biol, 59, 405-414.
Santos, F., Macdonald, G., Rubel, E.W. & Raible, D.W. 2006. Lateral line hair cell maturation is a determinant of aminoglycoside susceptibility in zebrafish (Danio rerio). Hear Res, 213, 25-33.
Scholik, A.R. & Yan, H.Y. 2002. The effects of noise on the auditory sensitivity of the bluegill sunfish, Lepomis macrochirus. Comp Biochem Physiol A, 133, 43-52.
Schönleber, J. & Anken, R.H. 2004. Efficacy of an ototoxic aminoglycoside (gentamicin) on the differentiation of the inner ear of cichlid fish. Adv Space Res, 33, 1416-1420.
Schuijf, A. 1975. Directional hearing of cod (Gadus morhua) under approximate free field conditions. Comp. J Physiol A, 98, 307-332.
Schuijf, A. & Buwalda, R.J.A. 1975. On the mechanism of directional hearing in cod (Gadus morhua L.). J Comp Physiol A, 98, 333-343.
Schuijf, A. & Hawkins, A.D. 1983. Acoustic distance discrimination by the cod. Nature, 302, 143-144.
Smith, M.E., Coffin, A.B., Miller, D.L. & Popper, A.N. 2006. Anatomical and functional recovery of the goldfish (Carassius auratus) ear following noise exposure. J Exp Biol, 209, 4193-4202.
Song, J., Young Yan, H. & Popper, A.N. 1995. Damage and recovery of hair cells in fish canal (but not superficial) neuromasts after gentamicin exposure. Hear Res, 91, 63-71.
Starr, A. & Achor, J. 1975. Auditory brain stem responses in neurological disease. Arch Neurol, 32, 761-768.
Wersäll, J., Bjorkroth, B., Flock, A. & Lundquist, P.-G. 1973. Experiments on ototoxic effects of antibiotics. Adv Oto-Rhino-Laryng, 20, 14-41.
Wysocki, L.E., Davidson, III J.W., Smith, M.E., Frankel, A.S., Ellison, W., et al. 2007. Effects of aquaculture production noise on hearing, growth, and disease resistance of rainbow trout Oncorhynchus mykiss. Aquaculture, 272, 687-697.
Yan, H.Y., Saidel,W.M., Chang, J.S., Presson, J.C. & Popper, A. N. 1991. Sensory hair cells of a fish ear: Evidence of multiple types based on ototoxicity sensitivity. Proc R Soc Lond B, 245, 133-138.