Antimicrobial resistance of Escherichia coli isolated from freshwaters and hospital effluents in Belgium

[en] The purpose of this work was to evaluate the level of antimicrobial resistant Escherichia coli isolates in freshwaters and hospital effluents in Belgium. The samples were collected from 24 locations along the Ourthe, Vesdre, Ambl eve and Meuse rivers and in the wastewater effluents of several hospitals. The sampling stations in rivers were classified according to the dominant land covers of the rivers (rural, urban and forest areas). Two sampling campaigns were organized in May and October 2019 to highlight a possible seasonal effect. A total of 938 E. coli strains were isolated on Chromogenic Selective Tryptone Bile X-glucuronide (TBX) and TBX supplemented with amoxicillin (TBX+AMX) media. Disk diffusion assays were performed following the EUCAST’s recommendations to assess the antimicrobial resistance against 12 antibiotics. A total of 32 7% of strains were at least resistant to one antibiotic and 24 6% were multiple antimicrobial resistant strains on TBX. The highest resistance rates were found for ampicillin (AMP), amoxicillin coupled with clavulanic acid (AMC) and sulfamethoxazole/trimethoprim (SXT). The lowest resistance rates were observed for meropenem (MEM) and ertapenem (ETP), which are last resort antibiotics. No significant difference was observed between both campaigns for the resistance rate to antibiotics.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Crettels, L.

Burlion, N.

Breyer, R.

Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Département des maladies infectieuses et parasitaires (DMI)

Servais, P.

Korfer, J.

Thiry, Damien ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie vétérinaire et maladies bactériennes animales

Language :

English

Title :

Antimicrobial resistance of Escherichia coli isolated from freshwaters and hospital effluents in Belgium

Publication date :

2021

Journal title :

Letters in Applied Microbiology

ISSN :

0266-8254

eISSN :

1472-765X

Publisher :

Blackwell, Oxford, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://mail.ulg.ac.be/service/home/~/?auth=co&loc=fr_FR&id=39186&part=4

Available on ORBi :

since 16 February 2022

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Bibliography

Bessa, L.J., Barbosa-Vasconcelos, A., Mendes, Â., Vaz-Pires, P. and Martins da Costa, P. (2014) High prevalence of multidrug-resistant Escherichia coli and Enterococcus spp. in river water, upstream and downstream of a wastewater treatment plant. J Water Health 12, 426–435.
Ghaderpour, A., Ho, W.S., Chew, L.-L., Bong, C.W., Chong, V.C., Thong, K.-L. and Chai, L.C. (2015) Diverse and abundant multi-drug resistant E. coli in Matang mangrove estuaries, Malaysia. Front Microbiol 6, 977.
Hassoun-Kheir, N., Stabholz, Y., Kreft, J.-U., de la Cruz, R., Romalde, J.L., Nesme, J., Sørensen, S.J., Smets, B.F. et al. (2020) Comparison of antibiotic-resistant bacteria and antibiotic resistance genes abundance in hospital and community wastewater: a systematic review. Sci Total Environ 743, 140804.
Herrig, I., Fleischmann, S., Regnery, J., Wesp, J., Reifferscheid, G. and Manz, W. (2020) Prevalence and seasonal dynamics of blaCTX-M antibiotic resistance genes and fecal indicator organisms in the lower Lahn River, Germany. PLoS One 15, e0232289.
Koczura, R., Mokracka, J., Barczak, A., Krysiak, N. and Kaznowski, A. (2013) Association between the presence of class 1 integrons, virulence genes, and phylogenetic groups of Escherichia coli isolates from river water. Microb Ecol 65, 84–90.
Krumperman, P.H. (1983) Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol 46, 165–170.
Kürekci, C., Aydin, M., Yipel, M., Katouli, M. and Gündoğdu, A. (2017) Characterization of extended spectrum β-lactamase (ESBL)-producing Escherichia coli in Asi (Orontes) River in Turkey. J Water Health 15, 788–798.
Lenart-Boroń, A. (2017) Antimicrobial resistance and prevalence of extended-spectrum beta-lactamase genes in Escherichia coli from major rivers in Podhale, southern Poland. Int J Environ Sci Technol 14, 241–250.
Matuschek, E., Brown, D.F.J. and Kahlmeter, G. (2014) Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories. Clin Microbiol Infect 20, O255–O266.
O’Dwyer, J., Hynds, P., Pot, M., Adley, C.C. and Ryan, M.P. (2017) Evaluation of levels of antibiotic resistance in groundwater-derived E. coli isolates in the Midwest of Ireland and elucidation of potential predictors of resistance. Hydrogeol J 25, 939–951.
Oberlé, K., Capdeville, M.-J., Berthe, T., Budzinski, H. and Petit, F. (2012) Evidence for a complex relationship between antibiotics and antibiotic-resistant Escherichia coli: from medical center patients to a receiving environment. Environ Sci Technol 46, 1859–1868.
Passerat, J., Tamtam, F., Le Bot, B., Eurin, J., Chevreuil, M. and Servais, P. (2010) Rejets hospitaliers d’antibiotiques et de bactéries fécales antibiorésistantes dans les rivières du bassin de la Seine. Eur J Water Qual 41, 1–13.
Pereira, A., Santos, A., Tacão, M., Alves, A., Henriques, I. and Correia, A. (2013) Genetic diversity and antimicrobial resistance of Escherichia coli from Tagus estuary (Portugal). Sci Total Environ 461–462, 65–71.
Pruden, A., Pei, R., Storteboom, H. and Carlson, K.H. (2006) Antibiotic resistance genes as emerging contaminants: studies in Northern Colorado. Environ Sci Technol 40, 7445–7450.
Ramírez Castillo, F.Y., Avelar González, F.J., Garneau, P., Márquez Díaz, F., Guerrero Barrera, A.L. and Harel, J. (2013) Presence of multi-drug resistant pathogenic Escherichia coli in the San Pedro River located in the State of Aguascalientes, Mexico. Front Microbiol 4, 147.
Sayah, R.S., Kaneene, J.B., Johnson, Y. and Miller, R. (2005) Patterns of antimicrobial resistance observed in Escherichia coli isolates obtained from domestic- and wild-animal fecal samples, human septage, and surface water. Appl Environ Microbiol 71, 1394–1404.
Servais, P. and Passerat, J. (2009) Antimicrobial resistance of fecal bacteria in waters of the Seine river watershed (France). Sci Total Environ 408, 365–372.
Su, H.-C., Ying, G.-G., Tao, R., Zhang, R.-Q., Zhao, J.-L. and Liu, Y.-S. (2012) Class 1 and 2 integrons, sul resistance genes and antibiotic resistance in Escherichia coli isolated from Dongjiang River, South China. Environ Pollut 169, 42–49.
Taylor, N.G.H., Verner-Jeffreys, D.W. and Baker-Austin, C. (2011) Aquatic systems: maintaining, mixing and mobilising antimicrobial resistance? Trends Ecol Evol 26, 278–284.
Zurfluh, K., Hächler, H., Nüesch-Inderbinen, M. and Stephan, R. (2013) Characteristics of extended-spectrum β-lactamase-and carbapenemase-producing Enterobacteriaceae isolates from rivers and lakes in Switzerland. Appl Environ Microbiol 79, 3021–3026.