Multiplex PCRs for Identification of Necrotoxigenic Escherichia coli

[en] Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necrotoxigenic E. coli strains isolated from different animal species.

Disciplines :

Microbiology

Author, co-author :

Van Bost, S.

Jacquemin, E.

Oswald, E.

Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes

Language :

English

Title :

Multiplex PCRs for Identification of Necrotoxigenic Escherichia coli

Publication date :

September 2003

Journal title :

Journal of Clinical Microbiology

ISSN :

0095-1137

eISSN :

1098-660X

Publisher :

Amer Soc Microbiology, Washington, United States - Washington

Volume :

Issue :

Pages :

4480-4482

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 27 October 2009

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Bibliography

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