Reference : Enteropathogenic and enterohaemorrhagic Escherichia coli deliver a novel effector cal...
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biochemistry, biophysics & molecular biology
Enteropathogenic and enterohaemorrhagic Escherichia coli deliver a novel effector called Cif, which blocks cell cycle G(2)/M transition
Marches, O. [> > > >]
Ledger, T. N. [> > > >]
Boury, M. [> > > >]
Ohara, M. [> > > >]
Tu, X. L. [> > > >]
Goffaux, F. [> > > >]
Mainil, Jacques mailto [Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]
Rosenshine, I. [> > > >]
Sugai, M. [> > > >]
De Rycke, J. [> > > >]
Oswald, E. [> > > >]
Molecular Microbiology
Blackwell Publishing Ltd
Yes (verified by ORBi)
[en] Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) are closely related pathogens. Both use a type III secretion system (TTSS) encoded by the 'locus of enterocyte effacement' (LEE) to subvert and attach to epithelial cells through the injection of a repertoire of effector molecules. Here, we report the identification of a new TTSS translocated effector molecule called Cif, which blocks cell cycle G(2)/M transition and induces the formation of stress fibres through the recruitment of focal adhesions. Cif is not encoded by the LEE but by a lambdoid prophage present in EPEC and EHEC. A cif mutant causes localized effacement of microvilli and intimately attaches to the host cell surface, but is defective in the ability to block mitosis. When expressed in TTSS competent LEE-positive pathogens, Cif is injected into the infected epithelial cells. These cells arrested at the G(2)/M phase displayed accumulation of inactive phosphorylated Cdk1. In conclusion, Cif is a new member of a growing family of bacterial cyclomodulins that subvert the host eukaryotic cell cycle.

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