Article (Scientific journals)
Comparative analysis of borate fusion versus sodium carbonate extraction for quantification of silicon contents in plants
Nakamura, R.; Cornelis, Jean-Thomas; de Tombeur, Félix et al.
2020In Journal of Plant Research, 133 (2), p. 271-277
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Keywords :
Borate fusion; ICP; Silicon; Sodium carbonate extraction; Tropical tree phytoliths; Borates; Carbonates; Chemical Fractionation; Plants
Abstract :
[en] Studies of plant-silicon (Si) interaction benefit from safe, affordable and accurate methods to measure acid-insoluble silica (phytoliths) for a large number of plant samples. This study aimed to evaluate the comparability between two chemical methods to dissolve leaf silica, borate fusion and 1% sodium carbonate (Na2CO3) extraction, in combination of two detection methods (ICP, molybdenum-blue colorimetry).We compared the results obtained by these methods, using dried leaf samples of five tropical tree species that differ widely in Si concentrations (4 to 100 mg g DW−1). Leaf Si concentration values determined after the two extraction methods were highly correlated (y = 0.79x, R2 = 0.998). However, compared to the extraction with borate fusion, the 1% Na2CO3 method resulted in lower Si concentration per unit dry mass by 16% to 32% (mean of 24.2%). We also found that molybdenum-blue colorimetry method may interfere with certain extraction methods. A simple equation can be used to correct for systematic underestimation of Si contents determined after extraction with 1% Na2CO3, which is the least expensive and safest among commonly used methods for extraction of Si from land plants. © 2020, The Botanical Society of Japan and Springer Japan KK, part of Springer Nature.
Disciplines :
Earth sciences & physical geography
Author, co-author :
Nakamura, R.;  Graduate School of Agriculture, Kyoto University, Kyoto, Japan
Cornelis, Jean-Thomas ;  Université de Liège - ULiège > Département GxABT > Echanges Eau - Sol - Plantes
de Tombeur, Félix ;  Université de Liège - ULiège > Département GxABT > Echanges Eau - Sol - Plantes
Nakagawa, M.;  Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
Kitajima, K.;  Graduate School of Agriculture, Kyoto University, Kyoto, Japan
Language :
English
Title :
Comparative analysis of borate fusion versus sodium carbonate extraction for quantification of silicon contents in plants
Publication date :
2020
Journal title :
Journal of Plant Research
ISSN :
0918-9440
eISSN :
1618-0860
Publisher :
Springer, Germany
Volume :
133
Issue :
2
Pages :
271-277
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 15 February 2022

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