[en] The possibility to continuously record locomotor movements using accelerometers (actigraphy) has allowed field studies of sleep and rest-activity patterns. It has also enabled large-scale data collections, opening new avenues for research. However, each brand of actigraph devices encodes recordings in its own format and closed-source proprietary softwares are typically used to read and analyse actigraphy data. In order to provide an alternative to these softwares, we developed a comprehensive open-source toolbox for actigraphy data analysis, pyActigraphy. It allows researchers to read actigraphy data from 7 different file formats and gives access to a variety of rest-activity rhythm variables, automatic sleep detection algorithms and more advanced signal processing techniques. Besides, in order to empower researchers and clinicians with respect to their analyses, we created a series of interactive tutorials that illustrate how to implement the key steps of typical actigraphy data analyses. As an open-source project, all kind of user’s contributions to our toolbox are welcome. As increasing evidence points to the predicting value of rest-activity patterns derived from actigraphy for brain integrity, we believe that the development of the pyActigraphy package will not only benefit the sleep and chronobiology research, but also the neuroscientific community at large.