Evaluation of hydroxyapatite texture using CTAB template and effects on protein adsorption

[en] This work aims to optimize the texture properties of hydroxyapatite (HA) in view to adopt them as biopharmaceutical drug delivery carrier. For this purpose, cetrimonium bromide (CTAB) has been adopted as a nanotemplate. The micellization behavior of this cationic surfactant has been studied within conditions simulating HA synthesis in order to better understand and control its aggregation behavior during HA synthesis. Micelle formation and their size have been monitored by dynamic light scattering (DLS) in the presence of phosphate ions, adopting different CTAB concentrations. Interestingly, distinct populations of CTAB aggregates have been distinguished in the autocorrelation curves derived from DLS analysis. The influence of CTAB aggregation during HA synthesis has been assessed by comparing the purified inorganic powders by FTIR, XRD, TGA, BET and TEM analysis. The evolution of the size and shape of HA nanoparticles visualized under TEM has been closely correlated to the change in specific surface area, which has considerably increased (up to 150 m2 .g− 1 ) in a narrow range of CTAB concentration. Soybean trypsin inhibitor (STI) was adopted as a model of bone morphogenic protein. Its loading capacity, release rate in vitro and its stability were correlated to the HA nanotexture.

Disciplines :

Materials science & engineering
Biotechnology

Author, co-author :

Monteiro, Ana ; Université de Liège - ULiège > CEIB

Idczak, Gaëlle; Université de Liège - ULiège

Tilkin, Rémi; Centre de recherches Centexbel

Vandeberg, Romain ; Université de Liège - ULiège > CEIB

Vertruyen, Bénédicte ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie inorganique structurale

Lambert, Stéphanie ; Université de Liège - ULiège > Department of Chemical Engineering > Nanomaterials, Catalysis, Electrochemistry

Grandfils, Christian ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine

Language :

English

Title :

Evaluation of hydroxyapatite texture using CTAB template and effects on protein adsorption

Publication date :

2021

Journal title :

Surfaces and Interfaces

eISSN :

2468-0230

Publisher :

Elsevier, Amsterdam, Netherlands

Volume :

Pages :

101565

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 19 January 2022

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