[en] Infrared-visible sum-frequency generation (SFG) spectroscopy is used to study the recognition of a protein (avidin) by a derivated vitamin (biocytin). No interaction is observed when the measurements are performed on metallic substrates in the normal co-propagative configuration. When the biocytin layer is deposited on a CaF2 substrate and the SFG measurements are performed in the total internal reflection configuration, drastic modifications in the C-H and N-H spectral ranges attest the molecular recognition of avidin by biocytin. The specificity of the recognition process is confirmed by replacing avidin by other proteins.
Disciplines :
Physics
Author, co-author :
Sartenaer, Y.
Dreesen, Laurent ; Université de Liège - ULiège > Département de physique > Biophotonique
Humbert, Christophe
Mani, A. A.
Lemaire, J.-J.
Salmain, M.
Pradier, C.-M.
Thiry, P. A.
Peremans, A.
Language :
English
Title :
Study by the sum-frequency generation spectroscopy of a model biosensor : The biocytin-avidin complex*
