[en] Vibrational information recorded by infrared-visible sum frequency generation spectroscopy was used to study the adsorption of a derivated vitamin (biocytin) on different substrates and its subsequent reaction with a protein (avidin). No reaction is observed on metallic substrates. When the experiments are carried out with a CaF2 substrate in the total internal reflection configuration, significant changes of the CH and NH vibrations can be related to the specific bonding of avidin to biocytin.
Disciplines :
Physics
Author, co-author :
Dreesen, Laurent ; Université de Liège - ULiège > Département de physique > Biophotonique
Sartenaer, Y.
Humbert, C.
Mani, A. A.
Lemaire, J.-J.
Méthivier, C.
Pradier, C.-M.
Thiry, P. A.
Peremans, A.
Language :
English
Title :
Sum-frequency generation spectroscopy applied to model biosensors systems
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