Sum-frequency generation spectroscopy applied to model biosensors systems

[en] Vibrational information recorded by infrared-visible sum frequency generation spectroscopy was used to study the adsorption of a derivated vitamin (biocytin) on different substrates and its subsequent reaction with a protein (avidin). No reaction is observed on metallic substrates. When the experiments are carried out with a CaF2 substrate in the total internal reflection configuration, significant changes of the CH and NH vibrations can be related to the specific bonding of avidin to biocytin.

Disciplines :

Physics

Author, co-author :

Dreesen, Laurent ; Université de Liège - ULiège > Département de physique > Biophotonique

Sartenaer, Y.

Humbert, C.

Mani, A. A.

Lemaire, J.-J.

Méthivier, C.

Pradier, C.-M.

Thiry, P. A.

Peremans, A.

Language :

English

Title :

Sum-frequency generation spectroscopy applied to model biosensors systems

Publication date :

2004

Journal title :

Thin Solid Films

ISSN :

0040-6090

Publisher :

Elsevier Science

Volume :

464-465

Pages :

373-378

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 October 2009

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