Article (Scientific journals)
Maximizing binary interactome mapping with a minimal number of assays.
Choi, Soon Gang; Olivet, Julien; Cassonnet, Patricia et al.
2019In Nature Communications, 10 (1), p. 3907
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Keywords :
Biological Assay/methods; Databases, Protein; HEK293 Cells; HeLa Cells; High-Throughput Screening Assays/methods; Humans; Protein Interaction Mapping/methods; Protein Interaction Maps; Proteins/metabolism; Proteome/analysis; Proteomics/methods; Two-Hybrid System Techniques
Abstract :
[en] Complementary assays are required to comprehensively map complex biological entities such as genomes, proteomes and interactome networks. However, how various assays can be optimally combined to approach completeness while maintaining high precision often remains unclear. Here, we propose a framework for binary protein-protein interaction (PPI) mapping based on optimally combining assays and/or assay versions to maximize detection of true positive interactions, while avoiding detection of random protein pairs. We have engineered a novel NanoLuc two-hybrid (N2H) system that integrates 12 different versions, differing by protein expression systems and tagging configurations. The resulting union of N2H versions recovers as many PPIs as 10 distinct assays combined. Thus, to further improve PPI mapping, developing alternative versions of existing assays might be as productive as designing completely new assays. Our findings should be applicable to systematic mapping of other biological landscapes.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Choi, Soon Gang
Olivet, Julien ;  Université de Liège - ULiège > GIGA
Cassonnet, Patricia
Vidalain, Pierre-Olivier
Luck, Katja
Lambourne, Luke
Spirohn, Kerstin
Lemmens, Irma
Dos Santos, Mélanie
Demeret, Caroline
Jones, Louis
Rangarajan, Sudharshan
Bian, Wenting
Coutant, Eloi P.
Janin, Yves L.
van der Werf, Sylvie
Trepte, Philipp
Wanker, Erich E.
De Las Rivas, Javier
Tavernier, Jan
Twizere, Jean-Claude  ;  Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies
Hao, Tong
Hill, David E.
Vidal, Marc
Calderwood, Michael A.
Jacob, Yves
More authors (16 more) Less
Language :
English
Title :
Maximizing binary interactome mapping with a minimal number of assays.
Publication date :
2019
Journal title :
Nature Communications
eISSN :
2041-1723
Publisher :
Nature Publishing Group, United Kingdom
Volume :
10
Issue :
1
Pages :
3907
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 December 2021

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