Foot-and-mouth disease virus non-structural protein 2B negatively regulates the RLR-mediated IFN-β induction

Evasion; Foot-and-mouth disease virus; Innate immune; Protein 2B; Type I IFNs; DDX58 protein, human; IFIH1 protein, human; Article; Foot and mouth disease virus; HEK293T cell line; IRF3 gene; TBK1 gene; HEK293 cell line; DEAD Box Protein 58; HEK293 Cells; Humans; Immunity, Innate; Interferon-beta; Interferon-Induced Helicase, IFIH1; Phosphorylation; Signal Transduction; Transfection; Up-Regulation; Viral Nonstructural Proteins

Abstract :

[en] Foot-and-mouth disease virus (FMDV) is the causative agent of Foot-and-mouth disease (FMD), which is an acute and highly contagious disease affecting pigs, cattle and other cloven-hoofed animals. Several studies have shown that FMDV has evolved multiple strategies to evade the host innate immune response, but the underlying mechanisms for immune evasion are still not fully understood. In the current research, we have demonstrated that FMDV utilizes its non-structural protein 2B to sabotage the host immune response. Over-expression of the FMDV 2B inhibited Poly(I:C)-induced or SeV-triggered up-regulation of IFN-β, IL-6 as well as ISG15. When HEK293T cells were transfected with FMDV 2B, the phosphorylation of TBK1 and IRF3 was inhibited. Co-immunoprecipitation and pull-down experiments indicated that FMDV 2B protein could interact with host RIG-I and MDA5. Moreover, FMDV 2B also inhibited the expression of the RIG-I and MDA5. Thus, FMDV 2B negatively regulates the RLR-mediated IFN-β induction by targeting RIG-I and MDA5. © 2018 Elsevier Inc.

Disciplines :

Agriculture & agronomy

Author, co-author :

Li, M.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

Xin, T.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

Gao, X.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

Wu, Jing ; Université de Liège - ULiège

Wang, X.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

Fang, L.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

Sui, X.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China, Gembloux Agro-bio Tech, University of Liège, Liège, 4000, Belgium

Zhu, H.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

Cui, S.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China, No.2, Yuanmingyuan West Road, Haidian District, Beijing, China

Guo, X.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

Language :

English

Title :

Foot-and-mouth disease virus non-structural protein 2B negatively regulates the RLR-mediated IFN-β induction

Publication date :

2018

Journal title :

Biochemical and Biophysical Research Communications

ISSN :

0006-291X

eISSN :

1090-2104

Publisher :

Elsevier, Atlanta, United States - California

Volume :

504

Issue :

Pages :

238-244

Peer reviewed :

Peer reviewed

Funders :

National Natural Science Foundation of China, NSFC: 31402232; National Basic Research Program of China (973 Program): 2017YFD0500902, 2017YFD0500906, 2017YFD0502302

Available on ORBi :

since 10 December 2021

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