Effects of Peptidic Glycosaminoglycans Complex on Human Chondrocytes Cultivated in Three Dimensions

[en] Human chondrocytes from the pelvic joint were cultivated in suspension; under these conditions, after a few days, cells aggregated. These chondrocytes were morphologically differentiated (round shape, situated inside cavities and surrounded by a matrix synthesized during cultivation) and biosynthetically differentiated (synthesis of type II collagen and cartilage proteoglycans (PG) (Bassleer et al. In vitro 22, 115-120, 1986). In this work, we present the metabolic and cellular effects of a peptidic-glycosaminoglycan (P-GAG) complex isolated from calf cartilage and bone marrow. We analyzed the effects of P-GAG on DNA synthesis (appreciated by 3H-thymidine incorporation into DNA), on type II collagen and on PG synthesis analyzed by specific radioimmunoassays. According to its final concentration in culture medium, P-GAG was able to stimulate proliferation or to favor the production of specific components of cartilage matrix, type II collagen and PG.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Bassleer, Corinne ; Université de Liège - ULiège

Gysen, Philippe ; Université de Liège - ULiège

Bassleer, Roger ; Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)

Franchimont, Paul

Language :

English

Title :

Effects of Peptidic Glycosaminoglycans Complex on Human Chondrocytes Cultivated in Three Dimensions

Publication date :

11 May 1988

Journal title :

Biochemical Pharmacology

ISSN :

0006-2952

eISSN :

1873-2968

Publisher :

Elsevier, Netherlands

Volume :

Issue :

Pages :

1939-45

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 06 December 2021

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