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Abstract :
[en] Plant Growth-Promoting Rhizobacteria (PGPR) induce systemic resistance (SR) in plants, decreasing the development of
phytopathogens. The FZB42 strain of Bacillus velezensis is known to induce an SR against pathogens in various plant species.
Previous studies suggested that it could also influence the interactions between plants and associated pests. However,
insects have developed several strategies to counteract plant defenses, including salivary proteins that allow the insect
escaping detection, manipulating defensive pathways to its advantage, deactivating early signaling processes, or detoxifying
secondary metabolites. Because Brown Marmorated Stink Bug (BMSB) Halyomorpha halys is highly invasive and
polyphagous, we hypothesized that it could detect the PGPR-induced systemic defenses in the plant, and efficiently adapt its
salivary compounds to counteract them. Therefore, we inoculated a beneficial rhizobacterium on Vicia faba roots and soil,
previous to plant infestation with BMSB. Salivary gland proteome of BMSB was analyzed by LC–MS/MS and a label-free
quantitative proteomic method. Among the differentially expressed proteins, most were up-regulated in salivary glands of
insects exposed to PGPR-treated plants for 24 h. We could confirm that BMSB was confronted with a stress during feeding
on PGPR-treated plants. The to-be-confirmed defensive state of the plant would have been rapidly detected by the invasive
H. halys pest, which consequently modified its salivary proteins. Among the up-regulated proteins, many could be associated
with a role in plant defense counteraction, and more especially in allelochemicals detoxification or sequestration.
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