Article (Scientific journals)
Host PDZ-containing proteins targeted by SARS-CoV-2.
Caillet-Saguy, Célia; Durbesson, Fabien; Rezelj, Veronica V. et al.
2021In FEBS Journal
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Keywords :
3A and N; PDZ-binding motif; PDZ-containing protein; SARS-CoV-2; human PDZ library; viral proteins E; viral replication
Abstract :
[en] Small linear motifs targeting protein interacting domains called PSD-95/Dlg/ZO-1 (PDZ) have been identified at the C terminus of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins E, 3a, and N. Using a high-throughput approach of affinity-profiling against the full human PDZome, we identified sixteen human PDZ binders of SARS-CoV-2 proteins E, 3A, and N showing significant interactions with dissociation constants values ranging from 3 to 82 μm. Six of them (TJP1, PTPN13, HTRA1, PARD3, MLLT4, LNX2) are also recognized by SARS-CoV while three (NHERF1, MAST2, RADIL) are specific to SARS-CoV-2 E protein. Most of these SARS-CoV-2 protein partners are involved in cellular junctions/polarity and could be also linked to evasion mechanisms of the immune responses during viral infection. Among the binders of the SARS-CoV-2 proteins E, 3a, or N, seven significantly affect viral replication under knock down gene expression in infected cells. This PDZ profiling identifying human proteins potentially targeted by SARS-CoV-2 can help to understand the multifactorial severity of COVID19 and to conceive effective anti-coronaviral agents for therapeutic purposes.
Disciplines :
Immunology & infectious disease
Author, co-author :
Caillet-Saguy, Célia
Durbesson, Fabien
Rezelj, Veronica V.
Gogl, Gergö
Tran, Quang Dinh
Twizere, Jean-Claude  ;  Université de Liège - ULiège > GIGA Molecul. Bio. of Diseases - Viral Interactomes Network
Vignuzzi, Marco
Vincentelli, Renaud
Wolff, Nicolas
Language :
English
Title :
Host PDZ-containing proteins targeted by SARS-CoV-2.
Publication date :
2021
Journal title :
FEBS Journal
ISSN :
1742-464X
eISSN :
1742-4658
Publisher :
Blackwell, Oxford, United Kingdom
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
© 2021 Federation of European Biochemical Societies.
Available on ORBi :
since 22 November 2021

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