ZRT-IRT-Like PROTEIN 6 expression perturbs local ion homeostasis in flowers and leads to anther indehiscence and male sterility.

Spielmann, Julien; Detry, Nathalie; Thiébaut, Noémie; Jadoul, Alice; Schloesser, Marie; Motte, Patrick; Périlleux, Claire; Hanikenne, Marc

doi:10.1111/pce.14200

Article (Scientific journals)

ZRT-IRT-Like PROTEIN 6 expression perturbs local ion homeostasis in flowers and leads to anther indehiscence and male sterility.

Spielmann, Julien; Detry, Nathalie; Thiébaut, Noémie et al.

2022 • In Plant, cell & environment, 45, p. 206-219

Peer reviewed

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https://hdl.handle.net/2268/264918

DOI
10.1111/pce.14200

PubMed
34628686

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Keywords :

anther indehiscence; endothecium; magnesium; male sterility; pollen; potassium; tapetum; zinc

Abstract :

[en] Metallic micronutrients are essential throughout the plant life cycle. Maintaining metal homeostasis in plant tissues requires a highly complex and finely tuned network controlling metal uptake, transport, distribution and storage. Zinc and cadmium hyperaccumulation, such as observed in the model plant Arabidopsis halleri, represents an extreme evolution of this network. Here, non-ectopic overexpression of the A. halleri ZIP6 (AhZIP6) gene, encoding a zinc and cadmium influx transporter, in Arabidopsis thaliana enabled examining the importance of zinc for flower development and reproduction. We show that AhZIP6 expression in flowers leads to male sterility resulting from anther indehiscence in a dose-dependent manner. The sterility phenotype is associated to delayed tapetum degradation and endothecium collapse, as well as increased magnesium and potassium accumulation and higher expression of the MHX gene in stamens. It is rescued by the co-expression of the zinc efflux transporter AhHMA4, linking the sterility phenotype to zinc homeostasis. Altogether, our results confirm that AhZIP6 is able to transport zinc in planta and highlight the importance of fine-tuning zinc homeostasis in reproductive organs. The study illustrates how the characterization of metal hyperaccumulation mechanisms can reveal key nodes and processes in the metal homeostasis network.

Disciplines :

Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Spielmann, Julien ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale

Detry, Nathalie

Thiébaut, Noémie ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale

Jadoul, Alice

Schloesser, Marie

Motte, Patrick ; Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale

Périlleux, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie végétale

Hanikenne, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Biologie végétale translationnelle

Language :

English

Title :

ZRT-IRT-Like PROTEIN 6 expression perturbs local ion homeostasis in flowers and leads to anther indehiscence and male sterility.

Publication date :

2022

Journal title :

Plant, cell & environment

ISSN :

0140-7791

eISSN :

1365-3040

Volume :

Pages :

206-219

Peer reviewed :

Peer reviewed

Commentary :

Available on ORBi :

since 09 November 2021

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