A Blood Exosomal miRNA Signature in Acute Respiratory Distress Syndrome.

[en] Acute respiratory distress syndrome (ARDS) is a diffuse, acute, inflammatory lung disease characterized by a severe respiratory failure. Recognizing and promptly treating ARDS is critical to combat the high mortality associated with the disease. Despite a significant progress in the treatment of ARDS, our ability to identify early patients and predict outcomes remains limited. The development of novel biomarkers is crucial. In this study, we profiled microRNA (miRNA) expression of plasma-derived exosomes in ARDS disease by small RNA sequencing. Sequencing of 8 ARDS patients and 10 healthy subjects (HSs) allowed to identify 12 differentially expressed exosomal miRNAs (adjusted p < 0.05). Pathway analysis of their predicted targets revealed enrichment in several biological processes in agreement with ARDS pathophysiology, such as inflammation, immune cell activation, and fibrosis. By quantitative RT-PCR, we validated the alteration of nine exosomal miRNAs in an independent cohort of 15 ARDS patients and 20 HSs, among which seven present high capability in discriminating ARDS patients from HSs (area under the curve > 0.8) (miR-130a-3p, miR-221-3p, miR-24-3p, miR-98-3p, Let-7d-3p, miR-1273a, and miR-193a-5p). These findings highlight exosomal miRNA dysregulation in the plasma of ARDS patients which provide promising diagnostic biomarkers and open new perspectives for the development of therapeutics.

Disciplines :

Cardiovascular & respiratory systems

Author, co-author :

Parzibut, Gilles

Henket, Monique

Moermans, Catherine

Struman, Ingrid ; Université de Liège - ULiège > GIGA Cancer - Molecular Angiogenesis Laboratory

Louis, Edouard ; Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie

Malaise, Michel ; Université de Liège - ULiège > Département des sciences cliniques > Rhumatologie

Louis, Renaud ; Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie

Misset, Benoît ; Université de Liège - ULiège > Département des sciences cliniques > Soins intensifs

NJOCK, Makon-Sébastien ^✱; Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de rhumatologie

Guiot, Julien ^✱; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie

^✱ These authors have contributed equally to this work.

Language :

English

Title :

A Blood Exosomal miRNA Signature in Acute Respiratory Distress Syndrome.

Publication date :

2021

Journal title :

Frontiers in Molecular Biosciences

eISSN :

2296-889X

Publisher :

Frontiers Media S.A., Lausanne, Switzerland

Volume :

Pages :

640042

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 29 September 2021

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