Streptomyces; TORK; common scab; lipopeptide; nonribosomal peptide; proteomics
Abstract :
[en] Streptomyces scabies is a phytopathogen associated with common scab disease. This is mainly attributed to its ability to produce the phytotoxin thaxtomin A, the biosynthesis of which is triggered by cellobiose. During a survey of other metabolites released in the presence of cellobiose, we discovered additional compounds in the thaxtomin-containing extract from Streptomyces scabies. Structural analysis by mass spectrometry (MS) and nuclear magnetic resonance (NMR) revealed that these compounds are amino acid sequence variants of the TOR (target of rapamycin) kinase (TORK) pathway inhibitory lipopeptide rotihibin A, and the main compounds were named rotihibins C and D. In contrast to thaxtomin, the production of rotihibins C and D was also elicited in the presence of glucose, indicating different regulation of their biosynthesis. Through a combination of shotgun and targeted proteomics, the putative rotihibin biosynthetic gene cluster rth was identified in the publicly available genome of S. scabies 87-22. This cluster spans 33 kbp and encodes 2 different nonribosomal peptide synthetases (NRPSs) and 12 additional enzymes. Homologous rth biosynthetic gene clusters were found in other publicly available and complete actinomycete genomes. Rotihibins C and D display herbicidal activity against Lemna minor and Arabidopsis thaliana at low concentrations, shown by monitoring the effects on growth and the maximal photochemistry efficiency of photosystem II.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège InBios - Integrative Biological Sciences - ULiège Laboratory for Microbiology - Department of Biochemistry and Microbiology - Ghent University NMR and Structure Analysis Group - Ghent University Laboratory of Applied Mycology and Phenomics - Department of Plants and Crops - Ghent University
Disciplines :
Microbiology
Author, co-author :
Planckaert, Sören
Deflandre, Benoit ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
de Vries, Anne-Mare
Ameye, Maarten
Martins, José
Audenaert, Kris
Rigali, Sébastien ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
Devreese, Bart
Language :
English
Title :
Identification of Novel Rotihibin Analogues in Streptomyces scabies, Including Discovery of its Biosynthetic Gene Cluster
Alternative titles :
[fr] Identification de nouveaux analogues des rotihibines et de leur cluster de gènes de biosynthèse chez Streptomyces scabies
Publication date :
04 August 2021
Journal title :
Microbiology Spectrum
eISSN :
2165-0497
Publisher :
American Society for Microbiology, United States
Volume :
9
Issue :
1
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] Ghent University research council
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