Article (Scientific journals)
Comparability of Raman Spectroscopic Configurations: A Large Scale Cross-Laboratory Study
Guo, Shuxia; Beleites, Claudia; Malherbe, Cédric et al.
2020In Analytical Chemistry, 92, p. 15745-15756
Peer Reviewed verified by ORBi
 

Files


Full Text
acs.analchem.0c02696.pdf
Publisher postprint (3.39 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Raman Spectroscopy; inter laboratory study; chemometrics; signal-to-noise; data processing; metrology
Abstract :
[en] The variable configuration of Raman spectroscopic platforms is one of the major obstacles in establishing Raman spectroscopy as a valuable physicochemical method within real-world scenarios such as clinical diagnostics. For such real world applications like diagnostic classification, the models should ideally be usable to predict data from different setups. Whether it is done by training a rugged model with data from many setups or by a primary-replica strategy where models are developed on a ‘primary’ setup and the test data are generated on ‘replicate’ setups, this is only possible if the Raman spectra from different setups are consistent, reproducible, and comparable. However, Raman spectra can be highly sensitive to the measurement conditions, and they change from setup to setup even if the same samples are measured. Although increasingly recognized as an issue, the dependence of the Raman spectra on the instrumental configuration is far from being fully understood and great effort is needed to address the resulting spectral variations and to correct for them. To make the severity of the situation clear, we present a round robin experiment investigating the comparability of 35 Raman spectroscopic devices with different configurations in 15 institutes within seven European countries from the COST (European Cooperation in Science and Technology) action Raman4clinics. The experiment was developed in a fashion that allows various instrumental configurations ranging from highly confocal setups to fibre-optic based systems with different excitation wavelengths. We illustrate the spectral variations caused by the instrumental configurations from the perspectives of peak shifts, intensity variations, peak widths, and noise levels. We conclude this contribution with recommendations that may help to improve the inter-laboratory studies.
Research center :
MolSys - Molecular Systems - ULiège
Disciplines :
Chemistry
Author, co-author :
Guo, Shuxia
Beleites, Claudia
Malherbe, Cédric ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Eppe, Gauthier  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Popp, Jürgen
Bocklitz, Thomas
Language :
English
Title :
Comparability of Raman Spectroscopic Configurations: A Large Scale Cross-Laboratory Study
Publication date :
2020
Journal title :
Analytical Chemistry
ISSN :
0003-2700
eISSN :
1520-6882
Publisher :
American Chemical Society, United States - District of Columbia
Volume :
92
Pages :
15745-15756
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 August 2021

Statistics


Number of views
36 (1 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
44
Scopus citations®
without self-citations
23
OpenCitations
 
29

Bibliography


Similar publications



Contact ORBi