PUC18-CpG stimulates RAW 264.7 via TBK1-mediated pathway and presents adjuvanticity in mice

Adjuvant; CpG DNA; Echinococcus granulosus; TBK1; Type I interferons; CPG-oligonucleotide; Tbk1 protein, mouse; Bagg albino mouse; RAW 264.7 cell line; Adjuvants, Immunologic; Animals; Antibodies, Helminth; Antigens, Helminth; Cytokines; Gene Expression Regulation; Immunity, Humoral; Mice; Mice, Inbred BALB C; Oligodeoxyribonucleotides; Protein-Serine-Threonine Kinases; RAW 264.7 Cells

Abstract :

[en] Phosphorothioate CpG oligodeoxynucleotides (ODN) are reported to be recognized by the membrane-bound TLR9 and trigger the MyD88-dependent up-regulation of Type I interferons and pro-inflammatory cytokines. Whether plasmids containing multiple CpG motifs stimulate the same signaling pathway is yet to be determined. The present results show that the CpG motifs enrich plasmid pUC18-CpG stimulates RAW 264.7 in vitro, mainly through the TBK1-mediated signaling pathway, causing the up-regulation of IFN-β, and pro-inflammatory cytokines TNF-α and IL-6. When pUC18-CpG is co-administered with the recombinant Echinococcus granulosus antigen, the antigen-specific antibody titers are markedly increased compared to the Quil-A adjuvanted group. Antigen specific cytokine quantification shows that cytokine profiles from the pUC18-CpG adjuvanted-group are switched to a Th1-biased immune response. © 2019 Polish Academy of Sciences. All rights reserved.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Wu, Jing ; Université de Liège - ULiège

Chen, Q.; College of Biological Science and Engineering, Beijing University of Agriculture, No. 7 Beinong Road, Haidian District, Beijing, 102206, China, Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture, No. 7 Beinong Road, Haidian District, Beijing, 102206, China

Xin, T.; Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China

Sun, Y.; China Animal Husbandry Industry Co., Ltd, No. 6 Huansan Road, Fengtai District, Beijing, 100070, China

Jia, H.; Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China

Hou, S. H.; Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China

Guo, X. Y.; Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China

Language :

English

Title :

PUC18-CpG stimulates RAW 264.7 via TBK1-mediated pathway and presents adjuvanticity in mice

Publication date :

2019

Journal title :

Polish Journal of Veterinary Sciences

ISSN :

1505-1773

Publisher :

HARD Pub. Co., Poland

Volume :

Issue :

Pages :

195-201

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 22 July 2021

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