Reference : PUC18-CpG stimulates RAW 264.7 via TBK1-mediated pathway and presents adjuvanticity i...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/262221
PUC18-CpG stimulates RAW 264.7 via TBK1-mediated pathway and presents adjuvanticity in mice
English
Wu, Jing [Université de Liège - ULiège > > >]
Chen, Q. [College of Biological Science and Engineering, Beijing University of Agriculture, No. 7 Beinong Road, Haidian District, Beijing, 102206, China, Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture, No. 7 Beinong Road, Haidian District, Beijing, 102206, China]
Xin, T. [Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China]
Sun, Y. [China Animal Husbandry Industry Co., Ltd, No. 6 Huansan Road, Fengtai District, Beijing, 100070, China]
Jia, H. [Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China]
Hou, S. H. [Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China]
Guo, X. Y. [Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China]
2019
Polish Journal of Veterinary Sciences
HARD Pub. Co.
22
2
195-201
Yes (verified by ORBi)
International
1505-1773
Poland
[en] Adjuvant ; CpG DNA ; Echinococcus granulosus ; TBK1 ; Type I interferons ; CPG-oligonucleotide ; Tbk1 protein, mouse ; Bagg albino mouse ; RAW 264.7 cell line ; Adjuvants, Immunologic ; Animals ; Antibodies, Helminth ; Antigens, Helminth ; Cytokines ; Gene Expression Regulation ; Immunity, Humoral ; Mice ; Mice, Inbred BALB C ; Oligodeoxyribonucleotides ; Protein-Serine-Threonine Kinases ; RAW 264.7 Cells
[en] Phosphorothioate CpG oligodeoxynucleotides (ODN) are reported to be recognized by the membrane-bound TLR9 and trigger the MyD88-dependent up-regulation of Type I interferons and pro-inflammatory cytokines. Whether plasmids containing multiple CpG motifs stimulate the same signaling pathway is yet to be determined. The present results show that the CpG motifs enrich plasmid pUC18-CpG stimulates RAW 264.7 in vitro, mainly through the TBK1-mediated signaling pathway, causing the up-regulation of IFN-β, and pro-inflammatory cytokines TNF-α and IL-6. When pUC18-CpG is co-administered with the recombinant Echinococcus granulosus antigen, the antigen-specific antibody titers are markedly increased compared to the Quil-A adjuvanted group. Antigen specific cytokine quantification shows that cytokine profiles from the pUC18-CpG adjuvanted-group are switched to a Th1-biased immune response. © 2019 Polish Academy of Sciences. All rights reserved.
http://hdl.handle.net/2268/262221
10.24425/pjvs.2019.127086

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