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Article (Scientific journals)
PUC18-CpG stimulates RAW 264.7 via TBK1-mediated pathway and presents adjuvanticity in mice
Wu, Jing  ; Chen, Q.; Xin, T. et al.
2019 • In Polish Journal of Veterinary Sciences, 22 (2), p. 195-201
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Keywords :
Adjuvant; CpG DNA; Echinococcus granulosus; TBK1; Type I interferons; CPG-oligonucleotide; Tbk1 protein, mouse; Bagg albino mouse; RAW 264.7 cell line; Adjuvants, Immunologic; Animals; Antibodies, Helminth; Antigens, Helminth; Cytokines; Gene Expression Regulation; Immunity, Humoral; Mice; Mice, Inbred BALB C; Oligodeoxyribonucleotides; Protein-Serine-Threonine Kinases; RAW 264.7 Cells
Abstract :
[en] Phosphorothioate CpG oligodeoxynucleotides (ODN) are reported to be recognized by the membrane-bound TLR9 and trigger the MyD88-dependent up-regulation of Type I interferons and pro-inflammatory cytokines. Whether plasmids containing multiple CpG motifs stimulate the same signaling pathway is yet to be determined. The present results show that the CpG motifs enrich plasmid pUC18-CpG stimulates RAW 264.7 in vitro, mainly through the TBK1-mediated signaling pathway, causing the up-regulation of IFN-β, and pro-inflammatory cytokines TNF-α and IL-6. When pUC18-CpG is co-administered with the recombinant Echinococcus granulosus antigen, the antigen-specific antibody titers are markedly increased compared to the Quil-A adjuvanted group. Antigen specific cytokine quantification shows that cytokine profiles from the pUC18-CpG adjuvanted-group are switched to a Th1-biased immune response. © 2019 Polish Academy of Sciences. All rights reserved.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Wu, Jing ;  Université de Liège - ULiège
Chen, Q.;  College of Biological Science and Engineering, Beijing University of Agriculture, No. 7 Beinong Road, Haidian District, Beijing, 102206, China, Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture, No. 7 Beinong Road, Haidian District, Beijing, 102206, China
Xin, T.;  Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China
Sun, Y.;  China Animal Husbandry Industry Co., Ltd, No. 6 Huansan Road, Fengtai District, Beijing, 100070, China
Jia, H.;  Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China
Hou, S. H.;  Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China
Guo, X. Y.;  Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China
Language :
English
Title :
PUC18-CpG stimulates RAW 264.7 via TBK1-mediated pathway and presents adjuvanticity in mice
Publication date :
2019
Journal title :
Polish Journal of Veterinary Sciences
ISSN :
1505-1773
Publisher :
HARD Pub. Co., Poland
Volume :
22
Issue :
2
Pages :
195-201
Peer reviewed :
Peer Reviewed verified by ORBi

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