ELF, human, magnetic fields; plasma melatonin; human exposure; urinary a-MT6s
Abstract :
[en] Concern about the health effects of extremely low frequency (ELF) magnetic fields (MF) has been raised by epidemiological studies indicating an association between certain cancers and living near power lines or working in high electric field environments. Alterations in pineal function have been proposed as a mechanism through which power-frequency MFs may interact with living organisms. A double blind laboratory study was performed to evaluate daytime exposure effects of 100 microT root mean square (rms) 50 Hz MF. Three head exposure sessions of 30 min each were performed: sham, continuous, and intermittent (15 s on/off cycles) MFs were presented to each subject in early or late afternoon (13:30 or 16:30 hr). Twenty-one healthy male volunteers (20-27 yr old) participated in these 3-weekly experimental conditions. Blood samples were drawn for serum melatonin measurement, hourly at night (from 20:00 to 07:00 hr) under controlled environmental conditions. Urinary excretion of 6-sulfatoxymelatonin (aMT6s), the main melatonin metabolite, was measured for a 17 hr period, by means of urine samples taken at 19:00 hr (14:00-19:00 hr "afternoon period"), 23:00 hr (19:00-23:00 hr "evening period"), and 07:00 hr, day 2 (23:00-07:00 hr day 2 "night-time period"). There were no significant differences in either plasma melatonin or in aMT6s excretion profiles in the three experimental conditions. However, a tendency for a smaller increase of night-time urinary aMT6s after continuous MF exposure was found (P=0.08) particularly in men with the lower excretion rate of aMT6s ("Low Group") (P=0.07). We conclude that this study does not indicate that daytime acute MF exposure influences either melatonin secretion or aMT6s excretion. Inter-individual differences in pineal production of melatonin, however, have to be taken into account in further studies.
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Crasson, Marion ; Université de Liège - ULiège > Département des sciences cliniques > Psychiatrie et psychologie médicale
Beckers, Véronique
Pequeux, Christel ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Claustrat, Bruno
Legros, Jean-Jacques ; Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Language :
English
Title :
Daytime 50 Hz Magnetic Field Exposure and Plasma Melatonin and Urinary 6-Sulfatoxymelatonin Concentration Profiles in Humans
Publication date :
October 2001
Journal title :
Journal of Pineal Research
ISSN :
0742-3098
eISSN :
1600-079X
Publisher :
Munksgaard International Publishers, Copenhagen, Denmark
Volume :
31
Issue :
3
Pages :
234-41
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Effets des champs électriques et magnétiques sur la santé, BBEMG
Arnetz B.B., Berg M. (1996) Melatonin and adrenocorticotropic hormone levels in Video Display Unit workers during work and leisure. Journal of Occupational and Environmental Medicine 38:1108-1110.
Bakos J., Nagy N., Thuroczy G., Szabo L.D. (1997) Urinary 6-sulfatoxymelatonin excretion is increased in rats after 24 hours of exposure to vertical 50-Hz, 100 μT magnetic field. Bioelectromagnetics 18:190-192.
Baldwin W.S., Barrett J.C. (1998) Melatonin: Receptor-mediated events that may affect breast and other steroid hormone-dependent cancers. Mol. Carcinogen. 21:149-155.
Bergiannaki J.D., Soldatos C.R., Papauigopoulos T.S., Syrengelas M., Stefanis C.N. (1995) Low and high melatonin excretors among healthy individual. J. Pineal Res. 18:159-164.
Bojklowski Ch.J., Arendt J., Shih M.C., Markey S.P. (1987) Melatonin secretion in humans assessed by measuring its metabolite, 6-sulfatoxymelatonin. Clin. Chem. 33:1343-1348.
Brzenzinski A. (1997) Melatonin in humans. N. Engl J. Med. 16:186-195.
Burch J.B., Reif J.S., Yost M.G. (1999) Geomagnetic disturbances are associated with reduced nocturnal excretion of a melatonin metabolite in humans. Neurosci. Lett. 266:209-212.
Burch J.B., Reif J.S., Yost M.G., Keefe T.J., Pitrat M.S. (1998) Nocturnal excretion of a urinary melatonin metabolite among electric utility workers. Scand. J. Work Environ. Health 24:183-189.
Claustrat B., Chazot G., Brun J., Jordan D., Sassolas G. (1984) A chronobiological study of melatonin and cortisol secretion in depressed subjects: Plasma melatonin, a biochemical marker in major depression. Biol. Psychiatry 19:1215-1228.
Cook M.R., Graham C., Cohen H.D., Gerkovich M.M. (1992) A replication study of human exposure to 60-Hz fields: Effects on neurobehavioral measures. Bioelectromagnetics 13:261-285.
Cook M.R., Graham C., Kavet R. (1997) Melatonin and its urinary metabolite: Comparison across age and gender. Second World Congress for Electricity and Magnetism in Biology and Medicine, Bologna, June 8-13 230 p.; .
Crasson M., Timsit-Berthier M., Legros J.J. (1992) Les champs èlectromagnétiques ont-ils un effet sur la santé? Revue de la litterature. Psychol. Méd. 24:1205-1215.
Crasson M., Timsit-Berthier M., Legros J.J. (1993) Contribution à l'étude des effets de l'exposition à des champs magnétiques 50-Hz sur certains paramètres neuropsychologiques et neuroendocriniens. Psychol. Méd. 25(13):1341-1346.
Crasson M., Legros J.J., Scarpa P., Legros W. (1999) 50 Hz magnetic field exposure influence on human performance and psychophysiological parameters. Two double-blind experimental studies. Bioelectromagnetics 20:474-486.
Graham C., Cook M.R., Riffle D.W. (1997) Human melatonin during continuous magnetic field exposure. Bioelectromagnetics 18:166-171.
Graham C., Cook M.R., Riffle D.W., Gerkovich M.M., Cohen H.D. (1996) Nocturnal melatonin levels in human volunteers exposed to intermittent 60-Hz magnetic fields. Bioelectromagnetics 17:263-273.
Graham C., Cook M.M., Kavet R., Smith D.K. (1998) Prediction of nocturnal plasma melatonin from morning urinary measures. J. Pineal Res. 24:230-238.
Graham C., Cook M.R., Sastre A., Riffle D.W., Gerkovich M.M. (2000) Multi-night exposure to 60 Hz magnetic fields: Effects on melatonin and its enzymatic metabolite. J. Pineal Res. 28:1-8.
Harthé C., Claustrat B., Brun J., Chazot G. (1991) Direct radioimmunoassay of 6-sulfatoxymelatonin in plasma with use of an iodinated tracer. Clin. Chem. 37:536-539.
(1998) Guidelines for limiting exposure to time-varying electric, magnetic, and electromagnetic fields (up to 300 GHz). Health Phys. 74:49-522.
Juutilainen J., Stevens R.G., Anderson L.E., Hansen N.H., Kumlin M., Laitinen J.T., Sobel E., Wilson B.W. (2000) Nocturnal 6-hydroxymelatonin sulfate excretion in female workers exposed to magnetic fields. J. Pineal Res. 28:97-104.
Karasek M., Woldanska-Okonska M., Czernicki J., Zylinska K., Swietoslawski J. (1998) Chronic exposure to 2.9 mT, 40 Hz magnetic field reduces melatonin concentrations in humans. J. Pineal Res. 25:240-244.
Karasek M., Czernickl J., Woldanska-Okonska M., Zylinska K., Swietoslawski J. (2000) Chronic exposure to 25-80 μT, 200 Hz magnetic field does not influence serum melatonin concentrations in patients with Iow back pain. J. Pineal Res. 29:81-85.
Lambrozo J., Touitou Y., Dab W. (1996) Exploring the EMF-melatonin connection: A review of the possible effects of 50/60-Hz electric and magnetic fields on melatonin secretion. Occup. Environ. Health 2:37-47.
Markey S.P., Higa S., Shih M., Danforth D.N., Tamarkin L. (1985) The correlation between human plasma melatonin levels and urinary 6-hydroxymelatonin excretion. Clin. Chim. Acta 150:221-225.
Niehaus M., Briiggemeyer H., Behre H.M., Lerchl A. (1997) Growth retardation, testicular stimulation, and increased melatonin synthesis by weak magnetic fields (50 Hz) in djungarian hamsters, Phodopus sungorus. Biochem, Biophys. Res. Commun. 234:707-711.
Pfluger D.H., Minder C.E. (1996) Effects of exposure to 16.7 Hz magnetic fields on urinary 6-hydroxymelatonin sulfate excretion of Swiss railway workers. J. Pineal Res. 21:91-100.
Portier C.J., Wolfe M.S. Assessment of health effects from exposure to power-line frequency electric and magnetic fields: NIEHS Working Group Report, NIH Publication No. 98-3981. NIH, Bethesda; 1998.
Portier C.J., Wolfe M.S. NIEHS report on health effects from exposure to power-line frequency electric and magnetic fields, NIH Publication No. 99-4493. NIH, Bethesda; 1999.
Reiter R.J. (1993) Static and extremely low frequency electromagnetic field exposure reported effects on the circadian production of melatonin. J. Cell. Biochem. 51:394-403.
Reiter R.J. (1995) Reported biological consequences related to the suppression of melatonin by electric and magnetic field exposure. Integr. Physiol. Behavi. Sci. 30:314-330.
Reiter R.J. (1998) Melatonin in the context of the reported bioeffects of environmental electromagnetic fields. Bioelectrochem. Bioenerg. 47:135-142.
Ronco A.L., Halberg F. (1996) The pineal gland and cancer. Anticancer Res. 16:2033-2039.
Schneider T., Semm P. (1992) The biological and possible clinical significance of magnetic influences on the pineal melatonin synthesis. Exp. Clin. Endocrinol. 11:251-258.
Selmaoui B., Lambrozo J., Touitou Y. (1996) Magnetic fields and pineal function in humans: Evaluation of nocturnal acute exposure to extremely low frequency magnetic fields on serum melatonin and urinary 6-sulfatoxymelatonin circadian rhythms. Life Sci. 58:1539-1549.
Stevens R.G. (1987) Electric power use and breast cancer: A hypothesis. Am. J. Epidemiol. 125:556-561.
Stevens R.G., Davis S., Thomas D.B., Anderson L.E., Wilson B.W. (1992) Electric power, pineal function, and the risk of breast cancer. FASEB J. 6:853-860.
Stevens R.G., Scott D. (1996) The melatonin hypothesis: Electric power and breast cancer. Environ..
Stevens R.G., Wilson B.W., Anderson L.E. The melatonin hypothesis. Breast cancer and use of electric Power, Battelle Press, Columbus; 1997.
Vaughan G.M., Bell R., De La Pena A. (1979) Nocturnal plasma melatonin in humans; episodic pattern and influence of light. Neurosci. Lett. 14:81-84.
Weitzman E.D., Weinberg U., D'Eletto R., Lynch H., Wurtman R.J., Czeisler C.H., Erlich S. (1978) Studies of the 24 hour rhythm of melatonin in man. J. Neural Transm. Suppl. 13:325-337.
Wetterberg L. (1978) Melatonin in humans. Physiological and clinical studies. J. Neural Transm. Suppl. 13:298-310.
Wilson B.W. (1988) Chronic exposure to ELF Fields may induce depression. Bioelectromagnetics 9:195-205.
Wilson B.W., Wright C.W., Morris J.E., Buschbom R.L., Brown D.L., SommersFlanigan R., Anderson L.E. (1990) Evidence for an effect of ELF electromagnetic fields on human pineal gland function. J. Pineal Res. 9:259-269.
Wilson B.W., Stevens R.G., Anderson L.E. (1989) Neuroendocrine mediated effects of electromagnetic field exposure: Possible role of the pineal gland. Life Sci. 45:1319-1332.
Wood A.W., Armstrong S.M., Sait M.L., Devine L., Martin M.J. (1998) Changes in human plasma melatonin profiles in response to 50 Hz magnetic field exposure. J. Pineal Res. 25:116-127.