Reference : Steatosis-induced proteomic changes in liver mitochondria evidenced by two-dimensiona...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Steatosis-induced proteomic changes in liver mitochondria evidenced by two-dimensional differential in-gel electrophoresis
Douette, Pierre [Université de Liège - ULiège > > Bioénergétique et physiologie cellulaire >]
Navet, Rachel mailto [Université de Liège - ULiège > > GIGA - Formation >]
Gerkens, Pascal [Université de Liège - ULiège > > > > Bioénergétique et physiologie cellulaire > >]
De Pauw, Edwin mailto [Université de Liège - ULiège > > Chimie physique, spectrométrie de masse >]
Leprince, Pierre mailto [Université de Liège - ULiège > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
Sluse-Goffart, Claudine [Université de Liège - ULiège > > > > Bioénergétique et physiologie cellulaire > >]
Sluse, Francis mailto [Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
Journal of Proteome Research
American Chemical Society
Yes (verified by ORBi)
[en] steatosis ; liver mitochondria ; proteomics ; two-dimensional differential in-gel electrophoresis ; ob/ob mouse
[en] Steatosis encompasses the accumulation of droplets of fats into hepatocytes. In this work, we performed a comparative analysis of mitochondrial protein patterns found in wild-type and steatosis-affected liver using the novel technique two-dimensional differential in-gel electrophoresis (2D-DIGE). A total of 56 proteins exhibiting significant difference in their abundances were unambiguously identified. Interestingly, major proteins that regulate generation and consumption of the acetyl-CoA pool were dramatically changed during steatosis. Many proteins involved in the response to oxidative stress were also affected.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Spéciaux de recherche dans les Universités ; Fonds Social Européen - FSE ; Région wallonne : Direction générale de l'Economie et de l'Emploi - DGEE ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
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