The coding and non-coding RNA single-cell atlas of the human fetal striatum

Dickinson Bocchi, V; Conforti, P; Bessuso, D; Cappadona, C; Ranzani, V; Bonnal, R.J.P.; De Simone, M; Rossetti, G; Espuny Camacho, Ira Mercedes; Faedo, A; Gervasoni, F; Vuono, R; He, X; Chen, J; Felsenfeld, D; Pavesi, G; Barker, R.A.; Pagani, M; Cattaneo, E

doi:10.1126/science.abf5759

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The coding and non-coding RNA single-cell atlas of the human fetal striatum

Dickinson Bocchi, V; Conforti, P; Bessuso, D et al.

2021 • In Science, 372 (6542)

Peer reviewed

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https://hdl.handle.net/2268/259450

DOI
10.1126/science.abf5759

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Keywords :

fetal striatum; single cell transcriptomics; long non-coding RNA

Abstract :

[en] Deciphering how the human striatum develops is paramount to understand diseases affecting this region. To decode the transcriptional modules that regulate this structure during development we first catalogued 1116, de novo identified, lincRNAs and then profiled 96,789 single-cells from the early human fetal striatum. We found that D1 and D2 medium spiny neurons (MSNs) arise from a common progenitor and that lineage commitment is established during the post-mitotic transition, across a pre-MSN phase that exhibits a continuous spectrum of fate determinants. We then uncovered cell type-specific gene regulatory networks that we validated through in silico perturbation. Finally, we identified human-specific lincRNAs that contribute to the phylogenetic divergence of this structure in humans. In conclusion, our study has delineated the cellular hierarchies governing MSN lineage commitment.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Dickinson Bocchi, V

Conforti, P

Bessuso, D

Cappadona, C

Ranzani, V

Bonnal, R.J.P.

De Simone, M

Rossetti, G

Espuny Camacho, Ira Mercedes ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

Faedo, A

More authors (9 more)

Language :

English

Title :

The coding and non-coding RNA single-cell atlas of the human fetal striatum

Publication date :

07 May 2021

Journal title :

Science

Volume :

372

Issue :

6542

Peer reviewed :

Peer reviewed

Commentary :

Bulk and single-cell RNA-seq reveal the human-specific modules governing human striatal development with the identification of previously unannotated cellular states and gene regulatory networks that define the ontogeny of medium spiny neurons.

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