Article (Scientific journals)
The coding and non-coding RNA single-cell atlas of the human fetal striatum
Dickinson Bocchi, V; Conforti, P; Bessuso, D et al.
2021In Science, 372 (6542)
Peer reviewed
 

Files


Full Text
Bocchi et al 2021 Science.pdf
Author postprint (45.17 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
fetal striatum; single cell transcriptomics; long non-coding RNA
Abstract :
[en] Deciphering how the human striatum develops is paramount to understand diseases affecting this region. To decode the transcriptional modules that regulate this structure during development we first catalogued 1116, de novo identified, lincRNAs and then profiled 96,789 single-cells from the early human fetal striatum. We found that D1 and D2 medium spiny neurons (MSNs) arise from a common progenitor and that lineage commitment is established during the post-mitotic transition, across a pre-MSN phase that exhibits a continuous spectrum of fate determinants. We then uncovered cell type-specific gene regulatory networks that we validated through in silico perturbation. Finally, we identified human-specific lincRNAs that contribute to the phylogenetic divergence of this structure in humans. In conclusion, our study has delineated the cellular hierarchies governing MSN lineage commitment.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Dickinson Bocchi, V
Conforti, P
Bessuso, D
Cappadona, C
Ranzani, V
Bonnal, R.J.P.
De Simone, M
Rossetti, G
Espuny Camacho, Ira Mercedes  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Faedo, A
Gervasoni, F
Vuono, R
He, X
Chen, J
Felsenfeld, D
Pavesi, G
Barker, R.A.
Pagani, M
Cattaneo, E
More authors (9 more) Less
Language :
English
Title :
The coding and non-coding RNA single-cell atlas of the human fetal striatum
Publication date :
07 May 2021
Journal title :
Science
Volume :
372
Issue :
6542
Peer reviewed :
Peer reviewed
Commentary :
Bulk and single-cell RNA-seq reveal the human-specific modules governing human striatal development with the identification of previously unannotated cellular states and gene regulatory networks that define the ontogeny of medium spiny neurons.
Available on ORBi :
since 30 April 2021

Statistics


Number of views
174 (18 by ULiège)
Number of downloads
111 (17 by ULiège)

Scopus citations®
 
30
Scopus citations®
without self-citations
22
OpenCitations
 
21

Bibliography


Similar publications



Contact ORBi