[en] High throughput sequencing was performed on virion-associated nucleic acids (VANA) from a pool of fifty asymptomatic rough bluegrasses (Poa trivialis L.) collected in a Belgian grazed pasture. Bioinformatics analyses produced some contigs presenting similarities with secovirid genomes, in particular nepoviruses and waikaviruses. Three distinct positive-sense single-stranded RNAs including 5’ and 3’ UTR were reconstructed and they represented two novel viruses infecting rough bluegrass, for which the provisional names poaceae Liege nepovirus A (PoLNVA, 7298 nt for RNA1 and 4263 nt for RNA2) and poaceae Liege virus 1 (PoLV1, 11,623 nt) were proposed. Compared to other Secoviridae members, the highest amino acid identity reached 90.7 % and 66.7 % between PoLNVA and nepoviruses for the Pro-Pol and CP regions respectively, while PoLV1 presented the highest amino acid identity with waikaviruses but with lower identities, i.e. 41.2 % for Pro-Pol and 25.8 % for CP regions, far below the ICTV demarcation criteria for novel secovirid. Based on sequence identity and phylogenetic analyses, PoLNVA was proposed to belong to the genus Nepovirus and PoLV1 as an unclassified secovirids. Detection of the two novel viruses was confirmed in high prevalence in rough bluegrass and ten other wild Poaceae species (Agropyron repens, Agrostis capillaris, Apera spica-venti, Anthoxanthum odoratum, Cynosorus cristatus, Festuca rubra, Holcus lanatus, Lolium perenne, Phleum bertolini and Phleum pratense) by RT-PCR and Sanger sequencing, revealing a diverse host range within Poaceae for these novel secovirids. Seed transmission was evaluated and confirmed for PoLNVA.
Alexander, H.M., Steets, J.A., Ali, A., Distribution of asclepias asymptomatic virus and exploration of possible effects on the wild plant host, asclepias viridis. Plant Health Prog., 21, 2020, 10.1094/PHP-09-19-0061-RS.
Altschul, S., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25 (1997), 3389–3402, 10.1093/nar/25.17.3389.
Awika, J.M., Major cereal grains production and use around the world. Advances in Cereal Science: Implications to Food Processing and Health Promotion, 2011, American Chemical Society, 1–13, 10.1021/bk-2011-1089 ch001.
Bankevich, A., Nurk, S., Antipov, D., Gurevich, A.A., Dvorkin, M., Kulikov, A.S., Lesin, V.M., Nikolenko, S.I., Pham, S., Prjibelski, A.D., Pyshkin, A.V., Sirotkin, A.V., Vyahhi, N., Tesler, G., Alekseyev, M.A., Pevzner, P.A., SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J. Comput. Biol. 19 (2012), 455–477, 10.1089/cmb.2012.0021.
Eweida, M., Rydén, K., Detection of barley yellow dwarf virus (BYDV) in cereals and grasses by enzyme-linked immunosorbent assay (ELISA) in Sweden / Nachweis des Virus der Gersten- Gelbverzwergung (BYDV) in Getreide und Gräsern durch ELISA in Schwede. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz / J. PlantDiseases Prot. 91 (1984), 131–137.
François, S., Filloux, D., Fernandez, E., Ogliastro, M., Roumagnac, P., Viral metagenomics approaches for high-resolution screening of multiplexed arthropod and plant viral communities. Methods in Molecular Biology, 2018, Humana Press Inc, 77–95, 10.1007/978-1-4939-7683-6_7.
Guy, P.L., Viruses of New Zealand pasture grasses and legumes: a review. Crop Pasture Sci., 65, 2014, 841, 10.1071/CP14017.
Ingwell, L.L., Bosque-Pérez, N.A., New experimental hosts of Barley yellow dwarf virus among wild grasses, with implications for grassland habitats. Plant Pathol. 64 (2015), 1300–1307, 10.1111/ppa.12387.
Jones, R.A.C., Virus diseases of pasture grasses in Australia: incidences, losses, epidemiology, and management. Crop Pasture Sci. 64 (2013), 216–233, 10.1071/CP13134.
Kraberger, S., Geering, A.D.W., Walters, M., Martin, D.P., Varsani, A., Novel mastreviruses identified in Australian wild rice. Virus Res. 238 (2017), 193–197, 10.1016/J.VIRUSRES.2017.07.003.
Lapierre, Signoret, Viruses and Virus Diseases of Poaceae (Gramineae). editi. ed., 2004, INRA, Paris, France.
Martin, D.P., Murrell, B., Golden, M., Khoosal, A., Muhire, B., RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evol., 1, 2015, 10.1093/ve/vev003.
Oñate-Sánchez, L., Vicente-Carbajosa, J., DNA-free RNA isolation protocols for Arabidopsis thaliana, including seeds and siliques. BMC Res. Notes 1 (2008), 1–7, 10.1186/1756-0500-1-93.
Ordon, F., Habekuss, A., Kastirr, U., Rabenstein, F., Kühne, T., Virus resistance in cereals: sources of resistance, genetics and breeding. J. Phytopathol. 157 (2009), 535–545, 10.1111/j.1439-0434.2009.01540.x.
Palanga, E., Filloux, D., Martin, D.P., Fernandez, E., Gargani, D., Ferdinand, R., Zabré, J., Bouda, Z., Neya, J.B., Sawadogo, M., Traore, O., Peterschmitt, M., Roumagnac, P., Metagenomic-based screening and molecular characterization of cowpea-infecting viruses in Burkina Faso. PLoS One, 11, 2016, 10.1371/journal.pone.0165188 e0165188.
Prendeville, H.R., Ye, X., Morris, T.J., Pilson, D., Virus infections in wild plant populations are both frequent and often unapparent. Am. J. Bot. 99 (2012), 1033–1042.
Rowhani, A., Daubert, S.D., Uyemoto, J.K., Al-Rwahnih, M., Fuchs, M., American nepoviruses. Meng, B., (eds.) Grapevine Viruses: Molecular Biology, Diagnostics and Management, 2017, Springer International Publishing AG, 109–126, 10.1007/978-3-319-57706-7.
Sanfaçon, H., Secoviridae: A Family of Plant Picorna-like Viruses With Monopartite or Bipartite Genomes. 2015, eLS, 1–14, 10.1002/9780470015902.a0000764.pub3.
Susi, P., Black currant reversion virus, a mite-transmitted nepovirus. Mol. Plant Pathol. 5 (2004), 167–173, 10.1111/j.1364-3703.2004.00217.x.
Thapa, V., Melcher, U., Wiley, G.B., Doust, A., Palmer, M.W., Roewe, K., Roe, B.A., Shen, G., Roossinck, M.J., Wang, Y.M., Kamath, N., Detection of members of the Secoviridae in the Tallgrass Prairie Preserve, Osage County, Oklahoma, USA. Virus Res. 167 (2012), 34–42, 10.1016/j.virusres.2012.03.016.
Thompson, J.R., Dasgupta, I., Fuchs, M., Iwanami, T., Karasev, A.V., Petrzik, K., Sanfaçon, H., Tzanetakis, I., van Der Vlugt, R., Wetzel, T., Yoshikawa, N., ICTV virus taxonomy profile: secoviridae. J. Gen. Virol. 98 (2017), 529–531, 10.1099/jgv.0.000779.
