Poster (Scientific congresses and symposiums)
Influence of storage temperature for respiratory epithelial samples on ciliary functional analysis
Benchimol, Lionel; Bricmont, Noémie; BOBOLI, Hedwige et al.
2021SRB ORL KBV: Springmeeting
 

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Keywords :
Primary Ciliary Dyskinesia; Diagnosis; Videomicroscopy; Sample processing
Abstract :
[en] Introduction and Aim: Primary ciliary dyskinesia is characterized by inherited stationary or dyskinetic respiratory cilia. Digital high speed videomicroscopy is highly sensitive and specific for diagnosis but lacks standardization. Particularly, the temperature for samples conservation has never been studied. The study goal was to compare ciliary analysis after samples storage at 4°C or 22°C. Agreement between two independent observers was sought as secondary goal. Material and Methods: Ciliated epithelial samples were obtained by inferior turbinate brushing from 13 healthy subjects, divided equally and conserved at 4°C or at 22°C. Digital high speed videomicroscopy assessed ciliary beat frequency (CBF) and percentage of normal ciliary beat pattern (CBP) nine hours after sampling. Results: There was no significant difference when samples were stored at 4°C or 22°C. Median CBF was 13.37 (12,23 –15,37) Hz and 15.24 (12,89 –16,06) Hz at 4°C and 22°C respectively (p = 0.210). Percentage of normal CBP was 81,80% (63,75 – 90,65) and 80,00% (71,70 – 87,30) at 4°C and 22°C respectively (p = 0.910). Moreover, inter observer analysis showed high agreement rate with 93,4% for the CBF and 90.0% for the CBP. Conclusion: This pilot study suggested with a high inter observer agreement rate that the temperature for respiratory ciliated samples conservation had no effect on ciliary function analysis. Larger studies are needed to confirm these preliminary results, notably in pathological conditions.
Disciplines :
Otolaryngology
Author, co-author :
Benchimol, Lionel ;  Centre Hospitalier Universitaire de Liège - CHU > Autres Services Médicaux > Service d'ORL, d'audiophonologie et de chir. cervico-faciale
Bricmont, Noémie ;  Université de Liège - ULiège > Département des sciences cliniques > Pédiatrie cardiologique et pneumologique
BOBOLI, Hedwige ;  Centre Hospitalier Universitaire de Liège - CHU > Département de Pédiatrie > Service de pédiatrie
SEGHAYE, Marie-Christine ;  Centre Hospitalier Universitaire de Liège - CHU > Département de Pédiatrie > Service de pédiatrie
LOUIS, Renaud ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de pneumologie - allergologie
LEFEBVRE, Philippe ;  Centre Hospitalier Universitaire de Liège - CHU > Autres Services Médicaux > Service d'ORL, d'audiophonologie et de chir. cervico-faciale
Papon, Jean-François;  Université Paris-Est > Faculté de Médecine > INSERM U955
Louis, Bernard;  Université Paris-Est > Faculté de Médecine > INSERM U955
Kempeneers, Céline  ;  Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
POIRRIER, Anne-Lise  ;  Centre Hospitalier Universitaire de Liège - CHU > Autres Services Médicaux > Service d'ORL, d'audiophonologie et de chir. cervico-faciale
Language :
English
Title :
Influence of storage temperature for respiratory epithelial samples on ciliary functional analysis
Publication date :
06 March 2021
Event name :
SRB ORL KBV: Springmeeting
Event organizer :
Royal Belgian Society for Oto-Rhino-Laryngology, Head and Neck Surgery
Event date :
6 mars 2021
Audience :
International
Available on ORBi :
since 18 March 2021

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