Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus.

Alkylation; Animals; Brucella abortus/genetics/metabolism; Brucellosis; DNA Damage/genetics; DNA Methylation/genetics; DNA Repair/genetics; Host-Pathogen Interactions/genetics; Macrophages/metabolism; Mice; RAW 264.7 Cells; Stress, Physiological/genetics; Vacuoles/metabolism

Abstract :

[en] It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.

Disciplines :

Immunology & infectious disease

Author, co-author :

Poncin, Katy

Roba, Agnès

Jimmidi, Ravikumar

Potemberg, Georges ; Centre Hospitalier Universitaire de Liège - CHU > Département de Physique Médicale > STA Assurance qualité

Fioravanti, Antonella

Francis, Nayla

Willemart, Kévin

Zeippen, Nicolas

Machelart, Arnaud

Biondi, Emanuele G.

More authors (3 more)

Language :

English

Title :

Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus.

Publication date :

2019

Journal title :

Nature Communications

eISSN :

2041-1723

Publisher :

Nature Publishing Group, United Kingdom

Volume :

Issue :

Pages :

4847

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 16 March 2021

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