Reference : Development and validation of a method for determining estrogenic compounds in surfac...
Scientific journals : Article
Life sciences : Environmental sciences & ecology
Physical, chemical, mathematical & earth Sciences : Chemistry
Development and validation of a method for determining estrogenic compounds in surface water at the ultra-trace level required by the EU Water Framework Directive Watch List
Glineur, Alex mailto [Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes >]
Nott, Katherine [La Société Wallonne des Eaux, > > Rue de la Concorde 41, 4800 Verviers, Belgium > >]
Carbonelle, Philippe [La Société Wallonie des Eaux > > > >]
Ronkart, Sebastien [La Société Wallonie des Eaux > > > >]
Purcaro, Giorgia mailto [Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes >]
Journal of Chromatography. A
Yes (verified by ORBi)
[en] Estrogenic compounds ; EU surface water Watch List ; LC-ESI-MS/MS ; SPE disk ; surface water ; method validation
[en] Natural estrogens (estrone: E1, 17 β-estradiol: E2, estriol: E3) and the synthetic estrogen (17 α- ethinylestradiol: EE2) are endocrine disruptors harmful to aquatic wildlife. The European Commission included these molecules in the surface water Watch Lists issued in 2015 and 2018 under the Water Framework Directive regarding emerging aquatic pollutants, proposing maximum detection limits (LOD) of 0.035 ng/L for EE2 and 0.4 ng/L for E1 and E2. Attaining these limits represents a challenge even with the most up-to-date analytical tools, in particular in surface water. A two-step sample preparation, involving a preliminary extraction of a whole water sample on a solid-phase extraction (SPE) disk and further purification on a Florisil SPE cartridge, was optimized. The purified extract was derivatized subsequently and quantified by LC-MS/MS. The main goal was to maximize the recoveries to achieve the very low LODs required by the European Watch Lists. The method was fully validated in seven surface water. The LODs calculated were below the maximum acceptable limits required by the European Commission.
Interreg France-Wallonie-Vlaanderen
DIADEM- Développement d’une Approche Intégrée pour le Diag- nostic de la qualitédes Eaux de la Meuse
Researchers ; Professionals

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