[en] BACKGROUND: Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry. RESULTS: In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculum densities was evaluated in fed-batch cultures. The greatest lipid content, 31% of CDW, was obtained using a low-density inoculum, a constant agitation rate of 800 rpm, and an oxygenation rate of 1.5 L/min. When the strain was cultured for 450 h in a chemostat containing a nitrogen-limited medium (dilution rate of 0.01 h(-1); 250 g/L crude glycerol), volumetric lipid productivity was 0.43 g/L/h and biomass yield was 60 g CDW/L. The coefficient of lipid yield to glycerol consumption (Y L/gly) and the coefficient of lipid yield to biomass yield (Y L/X ) were equal to 0.1 and 0.4, respectively. CONCLUSIONS: These results indicate that lipids may be produced using renewable feedstock, thus providing a means of decreasing the cost of biodiesel production. Furthermore, using molasses for biomass production and recycling glycerol from the biodiesel industry should allow biolipids to be sustainably produced.
Disciplines :
Biotechnology
Author, co-author :
Rakicka, Magdalena
Lazar, Zbigniew
Dulermo, Thierry
Fickers, Patrick ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Bio-industries
Nicaud, Jean Marc
Language :
English
Title :
Lipid production by the oleaginous yeast Yarrowia lipolytica using industrial by-products under different culture conditions.
Tai M, Stephanopoulos G (2013) Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production. Metab Eng 15:1-9
Hill J, Nelson E, Tilman D, Polasky S, Tiffany D (2006) Environmental, economic, and energetic costs and benefits of biodiesel and ethanol biofuels. Proc Natl Acad Sci USA 103:11206-11210
Papanikolaou S, Chevalot I, Komaitis M, Aggelis G, Marc I (2001) Kinetic profile of the cellular lipid composition in an oleaginous Yarrowia lipolytica capable of producing a cocoabutter substitute from industrial fats. Antonie Van Leeuwenhoek 80:215-224
Papanikolaou S, Chevalot I, Komaitis M, Marc I, Aggelis G (2002) Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures. Appl Microbiol Biotechnol 58:308-312
Meher LC, Sagar DV, Naik SN (2006) Technical aspects of biodiesel production by transesterification-a review. Renew Sust Energy Rev 10:248-268
Beopoulos A, Cescut J, Haddouche R, Uribelarrea JL, Molina-Jouve C, Nicaud JM (2009) Yarrowia lipolytica as a model for bio-oil production. Prog Lipid Res 48:375-387
Beopoulos A, Nicaud JM (2012) Yeast: a new oil producer. Ol Corps Gras Lipides OCL 19:22-88. doi: 10.1684/ocl.2012.0426
Thevenieau F, Nicaud J-M (2013) Microorganisms as sources of oils. Ol Corps Gras Lipides OCL 20(6):D603
Mliɥková K, Luo Y, Andrea S, Peɥ P, Chardot T, Nicaud JM (2004) Acyl-CoA oxidase, a key step for lipid accumulation in the yeast Yarrowia lipolytica. J Mol Catal B Enzym 28:81-85
Beopoulos A, Mrozova Z, Thevenieau F, Dall MT, Hapala I, Papanikolaou S et al (2008) Control of lipid accumulation in the yeast Yarrowia lipolytica. Appl Environ Microbiol 74:7779-7789
Blazeck J, Hill A, Liu L, Knight R, Miller J, Pan A et al (2014) Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production. Nat Commun 5:3131. doi: 10.1038/ncomms4131
Li Y, Zhao ZK, Bai F (2007) High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture. Enzyme Microb Tech 41:312-317
Zhao X, Kong X, Hua Y, Feng B, Zhao ZK (2008) Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi. Eur J Lipid Sci Technol 110:405-412
Meesters PAEP, Huijberts GNM, Eggink G (1996) High-cell-density cultivation of the lipid accumulating yeast Cryptococcus curvatus using glycerol as a carbon source. App Microbiol Biotechnol 45:575-579
Papanikolaou S, Aggelis G (2002) Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture. Bioresour Technol 82:43-49
Barth G, Gaillardin C (1996) Yarrowia lipolytica. In: Wolf K (ed) Nonconventional yeasts in biotechnology. Springer-Verlag, Berlin, Heidelberg, New York, pp 313-388
Xie D, Jackson EN (2015) Zhu Q Sustainable source of omega-3 eicosapentaenoic acid from metabolically engineered Yarrowia lipolytica: from fundamental research to commercial production. Appl Microbiol Biotechnol 99:1599-1610
Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497-509
Lazar Z, Dulermo T, Neuvéglise C, Crutz-LeCoq A-M, Nicaud J-M (2014) Hexokinase-a limiting factor in lipid production from fructose in Yarrowia lipolytica. Metab Eng 26:89-99
Joshi S, Bharucha C, Jha S, Yadav S, Nerurkar A, Desa AJ (2008) Biosurfactant production using molasses and whey under thermophilic conditions. Bioresour Technol 99:195-199
Makkar RS, Swaranjit S, Cameotra SS (1997) Utilization of molasses for biosurfactant production by two Bacillus strains at thermophilic conditions. J Am Oil Chem Soc 74:887-889
Babel W, Hofmann KH (1981) The conversion of triosephosphate via methylglyoxal, a bypass to the glycolytic sequence in methylotrophic yeasts? FEMS Microbiol Lett 10:133-136
Ermakova IT, Morgunov IG (1987) Pathways of glycerol metabolism in Yarrowia (Candida) lipolytica yeasts. Mikrobiology 57:533-537
May JW, Sloan J (1981) Glycerol utilization by Schizosaccharomyces pombe: dehydrogenation as the initial step. J Gen Microbiol 123:183-185
Makri A, Fakas S, Aggelis G (2010) Metabolic activities of biotechnological interest in Yarrowia lipolytica grown on glycerol in repeated batch cultures. Bioresour Technol 101:2351-2358
Bellou S, Makri A, Triantaphyllidou I-E, Papanikolaou S, Aggelis G (2014) Morphological and metabolic shifts of Yarrowia lipolytica induced by alteration of the dissolved oxygen concentration in the growth environment. Microbiology 160:807-817
Ykema A, Verbree EC, Verseveld HW, Smit H (1986) Mathematical modeling of lipid production by oleaginous yeast in continuous cultures. Antoinie Van Leeuwenhoek 52:491-506
Brown BD, Hsu KH, Hammond EG, Glatz BA (1989) A relationship between growth and lipid accumulation in Candida cyrvata D. J Ferment Bioeng 68:344-352
Ratledge C (1994) Yeast moulds algae and bacteria as sources of lipids. In: Kamel BS, Kakuda Y (eds) Technological advances in improved and alternative sources of lipids. Blackie academic and professional, London, pp 235-291
Evans CT, Ratledge C (1983) A comparison of the oleaginous yeast Candia curvata grown on different carbon sources in continuous and batch culture. Lipids 18:623-629
Rywińska A, Juszczyk P, Wojtatowicz M, Rymowicz W (2011) Chemostat study of citric acid production from glycerol by Yarrowia lipolytica. J Biotechnol 152:54-57
Davies RJ (1992) Scale up of yeast oil technology. In: Ratledge C, Kyle DJ (eds) Industrial application of single cell oil. AOCS Press, Champaign, pp 196-218
Meesters PAEP, van de Wal H, Weusthuis R, Eggink G (1996) Cultivation of the oleaginous yeast Carptococcus curvatus in a new reactor with improved mixing and mass transfer characteristics (surer®). Biotechnol Tech 10:277-282
Browse J, Mc Court PJ, Somerville CR (1986) Fatty acid composition of leaf lipids determined after combined digestion and fatty acid methyl ester formation from fresh tissue. Anal Biochem 152:141-145