Efficient D-threitol production by an engineered strain of Yarrowia lipolytica overexpressing xylitol dehydrogenase gene from Scheffersomyces stipitis

Chi, P.; Wang, S.; Ge, X.; Bilal, M.; Fickers, Patrick; Cheng, H.

doi:10.1016/j.bej.2019.107259

Article (Scientific journals)

Efficient D-threitol production by an engineered strain of Yarrowia lipolytica overexpressing xylitol dehydrogenase gene from Scheffersomyces stipitis

Chi, P.; Wang, S.; Ge, X. et al.

2019 • In Biochemical Engineering Journal, 149

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/257035

DOI
10.1016/j.bej.2019.107259

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Keywords :

Erythritol; Scheffersomyces stipitis; Threitol; Xylitol dehydrogenase; Yarrowia lipolytica; Candida; Glucose; Polyols; Sugar substitutes; Yeast; Gene encoding; Article; Candida parapsilosis

Abstract :

[en] D-threitol (hereinafter as threitol) is a diastereoisomer of erythritol with wider applications in green chemistry, food, pharmaceutical, and medicine. Herein, xylitol dehydrogenase from Scheffersomyces stipitis CBS 6054 was demonstrated able to catalyze highly efficient threitol formation from erythritol with erythrulose as an intermediate. When the corresponding gene Ss-XDH was overexpressed in the erythritol-producing Yarrowia lipolytica strain CGMCC7326, threitol was produced with a titer of 112 g·L−1 and yield of 0.37 from glucose. Unexpectedly, gene encoding mannitol dehydrogenase was upregulated in the threitol producing stain, leading to mannitol accumulation in the culture broth. In order to eliminate this byproduct and coproduced erythritol, a second process step based on the culture of Candida parapsilosis was developed to facilitate subsequent threitol purification. This is the first report to efficiently biosynthesize threitol from glucose through the fermentation process by an engineered Y. lipolytica and wild-type Candida parapsilosis. © 2019 Elsevier B.V.

Disciplines :

Biotechnology

Author, co-author :

Chi, P.; State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

Wang, S.; State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

Ge, X.; College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, China

Bilal, M.; School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China

Fickers, Patrick ; Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies

Cheng, H.; State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

Language :

English

Title :

Efficient D-threitol production by an engineered strain of Yarrowia lipolytica overexpressing xylitol dehydrogenase gene from Scheffersomyces stipitis

Publication date :

2019

Journal title :

Biochemical Engineering Journal

ISSN :

1369-703X

Publisher :

Elsevier B.V.

Volume :

149

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

NSCF - National Natural Science Foundation of China [CN]

Available on ORBi :

since 18 February 2021

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