Enhancing succinic acid productivity in the yeast Yarrowia lipolytica with improved glycerol uptake rate

Glycerol; Glycerol kinase; Overexpression; Productivity; Succinic acid; Yarrowia lipolytica; Cost effectiveness; Enzymes; Genes; Culture supernatant; Engineered strains; Feeding strategies; Gene over-expression; Over-expression; Succinic acids; Article; GUT1 gene; Saccharomyces cerevisiae; Yarrowia; Biological Transport; Bioreactors; Metabolic Engineering

Abstract :

[en] Development of cost effective and highly efficient process for bio-based succinic acid (SA) production is a main concern for industry. The metabolically engineered Y. lipolytica strain PGC01003 was successfully used for SA production with high titre. However, this strain possesses as main drawback with a low growth rate when glycerol is used as a feedstock. Herein, gene GUT1, encoding glycerol kinase, was overexpressed in strain PGC01003 with the aim to improve glycerol uptake capacity. In the resulting strain RIY420, glycerol uptake was 13.5% higher than for the parental strain. GUT1 gene overexpression also positively influences SA production. In batch bioreactor, SA titre, yield and productivity were 32%, 39% and 143% higher, respectively, than for the parental strain PGC01003. Using a glycerol feeding strategy, SA titre, yield and productivity were further improved by 11%, 5% and 10%, respectively. Moreover, the process duration to yield the highest concentration of SA in the culture supernatant was reduced by 9%. This demonstrated the contribution of metabolically engineered strain RIY420 to lower SA process cost and increase the efficiency of bio-based SA production. © 2019 Elsevier B.V.

Disciplines :

Biotechnology

Author, co-author :

Ong, K. L.; School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong

Fickers, Patrick ; Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies

Lin, C. S. K.; School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong

Language :

English

Title :

Enhancing succinic acid productivity in the yeast Yarrowia lipolytica with improved glycerol uptake rate

Publication date :

2020

Journal title :

Science of the Total Environment

ISSN :

0048-9697

eISSN :

1879-1026

Publisher :

Elsevier B.V.

Volume :

702

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

CityU - City University of Hong Kong

Available on ORBi :

since 18 February 2021

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