[en] Polysaccharides have received a lot of attention in biomedical research for their high potential as scaffolds owing to their unique biological properties. Fibrillar scaffolds made of chitosan demonstrated high promise in tissue engineering, especially for skin. As far as bone regeneration is concerned, curdlan (1,3-β-glucan) is particularly interesting as it enhances bone growth by helping mesenchymal stem cell adhesion, by favoring their differentiation into osteoblasts and by limiting the osteoclastic activity. Therefore, we aim to combine both chitosan and curdlan polysaccharides in a new scaffold for bone regeneration. For that purpose, curdlan was electrospun as a blend with chitosan into a fibrillar scaffold. We show that this novel scaffold is biodegradable (8% at two weeks), exhibits a good swelling behavior (350%) and is non-cytotoxic in vitro. In addition, the benefit of incorporating curdlan in the scaffold was demonstrated in a scratch assay that evidences the ability of curdlan to express its immunomodulatory properties by enhancing cell migration. Thus, these innovative electrospun curdlan–chitosan scaffolds show great potential for bone tissue engineering.
Research Center/Unit :
Complex and Entangled Systems from Atoms to Materials (CESAM) Research Unit Center for Education and Research on Macromolecules (CERM)
Disciplines :
Materials science & engineering Chemistry
Author, co-author :
Toullec, Clément ; University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM) Research Unit, Center for Education and Research on Macromolecules (CERM), Belgium > Universities of Angers and Nantes, CRCINA, SFR ICAT, France
Le Bideau, Jean; University of Nantes, CNRS, Institut des Matériaux Jean Rouxel, France
Geoffroy, Valérie; Université of Nantes, INSERM, Regenerative Medicine and Skeleton, ONIRIS, UFR Odontologie, France
Halgand, Boris; Université of Nantes, INSERM, Regenerative Medicine and Skeleton, ONIRIS, UFR Odontologie, France > CHU Nantes, France
Buchtova, Nela; Universities of Angers and Nantes, CRCINA, SFR ICAT, France
Molina‐Peña, Rodolfo; Universities of Angers and Nantes, CRCINA, SFR ICAT, France
Garcion, Emmanuel; Universities of Angers and Nantes, CRCINA, SFR ICAT, France
Avril, Sylvie; Universities of Angers and Nantes, CRCINA, SFR ICAT, France
Sindji, Laurence; Universities of Angers and Nantes, CRCINA, SFR ICAT, France
Dube, Admire; University of the Western Cape, School of Pharmacy, South Africa
Boury, Frank; Universities of Angers and Nantes, CRCINA, SFR ICAT, France
Jérôme, Christine ; University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM) Research Unit, Center for Education and Research on Macromolecules (CERM), Belgium
Language :
English
Title :
Curdlan–chitosan electrospun fibers as potential scaffolds for bone regeneration
Publication date :
02 February 2021
Journal title :
Polymers
ISSN :
2073-4360
Publisher :
MDPI Open Access Publishing, Switzerland
Volume :
13
Issue :
4
Pages :
526
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Ligue Nationale Contre le Cancer The french "Comité Départemental de Loire‐Altantique" The french "Région Pays‐de‐la‐Loire" FEDER - Fonds Européen de Développement Régional Walloon region the PROSTEM2 project
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