Article (Scientific journals)
β-arrestin2 recruitment at the β2 adrenergic receptor: a luciferase complementation assay adapted for undergraduate training in pharmacology
Ferraiolo, Mattia; Beckers, Pauline; Marquet, Nicolas et al.
2021In Pharmacology Research and Perspectives, 9 (1)
Peer Reviewed verified by ORBi
 

Files


Full Text
Article_Ferraiolo et al_Arrestin assay for pharmacology training.pdf
Publisher postprint (1.08 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Adrenoceptors; G protein coupled recepto; Arrestin
Abstract :
[en] In the context of pharmacology teaching, hands-on activities constitute an essential complement to theoretical lectures. Frequently, these activities consist in exposing fresh animal tissues or even living animals to selected drugs and qualitatively or quantitatively evaluating functional responses. However, technological advancements in pharmacological research and the growing concerns for animal experimentation support the need for innovative and flexible in vitro assays adapted for teaching purposes. We herein report the implementation of a luciferase complementation assay enabling to dynamically monitor β-arrestin2 recruitment at the β2 adrenergic receptor in the framework of pharmacological training at the faculty of Pharmacy and Biomedical Sciences. The assay allowed students to quantitatively characterize the competitive antagonism of propranolol, and to calculate pEC50, pKB and pA2 values after a guided data-analysis session. Moreover, the newly implemented workshop delivered highly reproducible results and were generally appreciated by students. As such, we report that the luciferase complementation-based assay proved to be a straightforward, robust and cost-effective alternative to experiments performed on animal tissues, constituting a useful and flexible tool to enhance and update current hands-on training in the context of pharmacological teaching.
Disciplines :
Education & instruction
Pharmacy, pharmacology & toxicology
Author, co-author :
Ferraiolo, Mattia;  Université Catholique de Louvain - UCL > Institute of Neuroscience > Neuropharmacology Laboratory
Beckers, Pauline
Marquet, Nicolas
Roumain, Martin
Ruiz, Lucie
Dupuis, Nadine
Hanson, Julien  ;  Université de Liège - ULiège > GIGA - Molecular Biology of Diseases > Laboratory of Molecular Pharmacology
Hermans, Emmanuel;  Université Catholique de Louvain - UCL > Institute of Neuroscience > Neuropharmacology Laboratory
Language :
English
Title :
β-arrestin2 recruitment at the β2 adrenergic receptor: a luciferase complementation assay adapted for undergraduate training in pharmacology
Publication date :
February 2021
Journal title :
Pharmacology Research and Perspectives
eISSN :
2052-1707
Publisher :
John Wiley & Sons
Volume :
9
Issue :
1
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 03 February 2021

Statistics


Number of views
137 (4 by ULiège)
Number of downloads
132 (2 by ULiège)

Scopus citations®
 
1
Scopus citations®
without self-citations
1
OpenCitations
 
0

Bibliography


Similar publications



Contact ORBi