Rats; Time Factors; Androgen Antagonists; Animals; Sexual Maturation; Cephalometry; Testosterone; Maxillofacial Development; Orchiectomy
Abstract :
[en] The role of endogenous testosterone in the craniofacial growth of the young male rat was investigated. First, the effect of neonatal surgical castration was examined in a randomized, cross-sectional study in which male Wistar rats were allocated to be either castrated or sham-operated 4 h after birth. Then, the effect of prepubertal chemical castration was analysed in a second, randomized longitudinal study in which male Wistar rats were randomly allocated either to a control group or to two experimental groups, one injected with triptorelin at day 25 and the other injected on day 25 and on day 45. Every tenth day between 20 and 70 days of age for the first study, and between 30 and 110 days of age for the second, body length and weight were measured, cephalometric X-rays taken, and blood samples obtained. Neonatal and prepubertal castration resulted in decreased plasma concentrations of testosterone and in delayed growth of somatic and craniofacial components. The initiation, duration and magnitude of the effect was dependent on individual bones (cranial base, skull roof) and on the lower incisor, and related to the testosterone concentrations. These results suggest that testosterone effects participate in the process of normal craniofacial growth, particularly during puberty.
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Verdonck, A.; School of Dentistry, Department of Orthodontics, University Hospital St. Rafaël, Leuven, Belgium
De Ridder, L.; School of Dentistry, Department of Orthodontics, University Hospital St. Rafaël, Leuven, Belgium
Verbeke, G.
Bourguignon, Jean-Pierre ; Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Carels, C.; School of Dentistry, Department of Orthodontics, University Hospital St. Rafaël, Leuven, Belgium
Kühn, E. R.
Darras, V.
De Zegher, F.
Language :
English
Title :
Comparative effects of neonatal and prepubertal castration on craniofacial growth in rats
Asch R.H., Rojas F.J., Andrzej B., Schally A.V., Tice T.R., Klemcke H.G., Siler-Khodr Tm, Bray R.E., Hogan M.P. Prolonged suppression of plasma LH levels in male rats after a single injection of an L-RH agonist in poly (DL-lactide-co-glycolide) microcapsules. J. Androl. 6:1984;83-88.
Attie K.M., Ramirez N.R., Conte F.A., Kaplan S.L., Grumbach M.M. The pubertal growth spurt in eight patients with true precocious puberty and growth hormone deficiency: evidence for a direct role of sex steroids. J. Clin. Endocrinol. Metab. 71:1990;975-983.
Baes M., Denef C. Evidence that stimulation of growth hormone release by epinephrine and vasoactive intestinal peptide is based on cell-to-cell communication in the pituitary. Endocrinology. 120:1987;280-284.
Baughan, B., Demirjian, A., Levesque, G.Y., La, Palme-Chaput, L., 1979. The pattern of facial growth before and during puberty as shown by French-Canadian girls. Ann. Hum. Biol. 59-76.
Bourguignon J.P., Gerard A., Mathieu J., Mathieu A., Franchimont P. Maturation of the hypothalamic control of pulsatile gonadotropin-releasing hormone secretion at onset of puberty. Increased activation of N-methyl-D-aspartate receptors. Endocrinology. 127:1990;873-881.
Bourguignon, J.P., Gerard, A., Fawe, L., Alvarez-Gonzalez, M.L., Franchimont, P., 1991.Neuroendocrine control of the onset of puberty: secretion of gonadotrophin-releasing hormone from rat hypothalamic explants. Acta Paediatr. Scand. (Suppl. 372), 19-25.
Bourguignon, J.P., 1993. Delayed puberty and hypogonadism. In: J. Bertrand, R. Rappaport, P. Sizonenko (Ed.). Pediatric Endocrinology. Physiology, Pathophysiology and Clinical Aspects. Williams and Wilkins, Baltimore, pp. 404-419.
Brown B.C., Butler G.E., Kelnar C.J. A double blind, placebo controlled study of the effects of low dose testosterone undecanoate on the growth of small for age, prepubertal boys. Arch. Dis. Child. 73:1995;131-135.
Cleall J.F., Wilson G.W., Garnett D.S. Normal craniofacial skeletal growth of the rat. Am. J. Phys. Antrop. 29:1969;225-242.
Corvol M.T., Carrascosa A., Tsadris L., Blanchard O., Rappaport R. Evidence for a direct in vitro action of sex steroids on rabbit cartilage cells during skeletal growth:influence of age and sex. Endocrinology. 120:1987;1422-1429.
de Zegher F., Maes M., Gargosky S.E., Heinrichs C., Du Caju M.V.L., Thiry G., De Schepper J., Craen M., Breysem L., Löfström A., Jönsson P., Bourguignon J.P., Malvaux P., Rosenfeld R.G. High dose growth hormone treatment of short children born small for gestational age. J. Clin. Endocrinol. Metab. 81:1996;1887-1892.
Dohler K.D., Wuttke W. Changes with age in levels of serum gonadotropins, prolactin, and gonadal steroids in prepubertal male and female rats. Endocrinology. 97:1975;898-907.
Duncker M., Tönjes R., Rothe C., Scherkenbach C., Zglinicki T. Experimentelle Untersuchungen zum Einfluss von Testosteron auf die Schädelmorphogenese der Ratte. Fortschr Kieferorthop. 49:1988;342-357.
Eden S., Albertsson-Wikland K., Isaksson O. Plasma levels of growth hormone in female rats at different ages. Acta Endocrinol. Copenh. 88:1978;676-690.
Eden S. Age- and sex-related differences in episodic growth hormone secretion in the rat. Endocrinology. 105:1979;555-560.
Engström C., Linde A., Thilander B. Craniofacial morphology and growth in the rat. Cephalometric analysis of the effects of a low calcium and vitamin D-deficient diet. J. Anat. 134:1982;299-314.
Fishman L.S. Radiographic evaluation of skeletal maturation. Angle Orthod. 52:1982;88-122.
Grave K.C. Timing of facial growth: a study of relations with stature and ossification in the hand around puberty. Austr. Ortho. J. 3:1973;117-122.
Hybrechts L.M., De Cupere E., Kuhn E.R., Lauterio T.J. Growth hormone secretory response to TRH in normal and dwarf chickens. Reprod. Nutr. Dev. 25:1985;641-645.
Jansson J.O., Eden S., Isaksson O. Sites of action of testosterone and estradiol on longitudinal bone growth. Am. J. Physiol. 243:1983;E135-E140.
Jansson J.O., Ekberg S., Isaksson O.G.P., Eden S. Influence of gonadal steroids on age- and sex-related secretory patterns of growth hormone in the rat. Endocrinology. 114/4:1984;1287-1294.
Jansson J.O., Ekberg S., Isaksson O., Mode A., Gustafsson J.A. Imprinting of growth hormone secretion, body growth, and hepatic steroid metabolism by neonatal testosterone. Endocrinology. 177/5:1985(a);1881-1889.
Jansson J.O., Eden S., Isaksson O. Sexual dimorphism in the control of growth hormone secretion. Endoc. Rev. 6:1985(b);128-150.
Kasperk C., Wergedal J.E., Farley J.R., Linkkhart T.A., Turner R.T., Baylink D. Androgens directly stimulate proliferation of bone cells in vitro. Endocrinology. 124:1989;1576-1579.
Kasperk C., Fitzsimmons R., Strong D., Mohan S., Jenning J., Wergedal J., Baylink D. Studies of the mechanism by which androgens enhance mitogenesis and differentiation in bone cells. J. Clin. Endocrinol. Metab. 71:1990;1322-1329.
Keenan B.S., Richards G.E., Ponder S.W., Dallas J.S., Nagamani M., Smith E.R. Androgen-stimulated pubertal growth: the effects of testosterone and dihydrotestosterone on growth hormone and insuline-like growth factor-I in the treatment of short stature and delayed puberty. J. Clin. Endocrinol. Metab. 76:1993;996-1001.
Kerrigan J.R., Rogol A.D. The impact of gonadal steroid hormone action on growth hormone secretion during childhood and adolescence. Endocr. Rev. 13:(2):1992;281-298.
Killiany D.M., Johnson O.N., Jonhston L.E. Combined cephalometric and transcranial radiography of the rat condyle. Angle Orthod. 57:1987;162-167.
Kiliaridis S., Engström C., Thilander B. The relationship between masticatory function and craniofacial morphology. I. A cephalometric longitudinal analysis in the growing rat fed a soft diet. Eur. J. Orthod. 7:1985;273-283.
Korach K.S. Insights from the study of animals lacking functional estrogen receptor. Science. 266:1994;1524-1527.
Laron Z., Sarel R., Pertzelan A. Puberty in Laron type dwarfism. Eur. J. Pediatr. 134:1980;79-83.
Morishima A., Grumbach M.M., Simpson E.R., Fisher C., Qin K. Aromatase deficiency in male and female siblings caused by a novel mutation and the physiological role of estrogens. J. Clin. Endocrinol. Metab. 80:1995;3689-3698.
Moss L.M., Baer M.J. Differential growth of the rat skull. Growth. 20:1956;107-120.
Persson E.C., Engström C., Thilander B. The effect of thyroxine on craniofacial morphology in the growing rat. Part I: a longitudinal cephalometric analysis. Eur. J. Orthod. 11:1989;59-66.
Piascek B.E., Goodspeed M.P. Maturation of the pituitary-gonadal system in the male rat. J. Reprod. Fert. 52:1978;29-35.
Redding T.W., Schally A.V., Tice T.R., Meyers W.E. Long-acting delivery systems for peptides: inhibition of rat prostate tumors by controlled release of (D-Trp) luteinizing hormone-releasing hormone from injectable microcapsules. Proc. Natl. Acad. Sci. 81:1984;5845-5848.
Reitsma J.W. Het merken van laboratorium dieren. Biotechniek. 2:1963;135-143.
Renaville R., Massart S., Lognay G., Devolder A., Sneyers M., Marlier M., Severin M., Burny A., Portetelle D. Influence of a hormonal preparation containing glucocorticoids (dexamethasone esters), progestagen (chloramidone acetate) and oestrogen (ethinyl oestradiol) on testerone, insuline-like growth factor-I (IFG-I), IGF-binding proteins and spermatogenic cells in finishing bulls. Anim. Prod. 59:1994;189-196.
Riesenfeld, A. Endocrine and biochemical control of craniofacial growth: an experimental study. Hum. Biol. 46:1974;531-572.
Schoutens A., Verhas M., L'Hermite-Baleriaux, L'Hermite M., Verschaeren A., Dourov N., Mone M., Heilporn A., Tricot A. Growth and bone haemodynamic responses to castration in male rats. Reversibility by testosterone. Acta Endocrinol. 107:1984;428-432.
Smith E.P., Boyd J., Frank G.R., Takahashi H., Cohen R.M., Specker B., Williams T.C., Lubahn D.B., Korach K.S. Estrogen resistance caused by a mutation in the estrogen-receptor gene in a man. N. Engl. J. Med. 331:(16):1994;1056-1061.
Stanhope R., Bommen M., Brook C.G.D. Constitutional delay of growth and puberty in boys: the effect of a short course of treatment with fluoxymesterone. Acta Paediatrica Scandinavica. 74:1985;390-393.
Stutzmann, J.J., Petrovic, A.J., 1978. Der Einfluss von Testosteron auf die Wachstumsgeschwindigkeit des Kondylenknorpels der jungen Ratte. Die Rolle des "Vergleichers" des Servosystems, welches das Längenwachstum des Unterkiefers kontrolliert, 39. Fortschr Kieferorthop., pp. 345-362.
Symons A.L., Paxinos D., Leong K., Waters M.J., Seymour G.J. Effect of growth hormone on odontogenesis in the rat. J. Dent. Res. 74:1995;456.
Tanner J.M., Whitehouse R.H., Hughes P.C.R., Carter B.S. Relative importance of growth hormone and sex steroids for the growth at puberty of trunk length, limb length, and muscle width in growth hormone deficient children. J. Pediatr. 89:1976;1000.
Thompson G.W., Popovich F. Relationship of craniofacial changes and skeletal increment in females. Hum. Biol. 45:1973;595-603.
Turner R.T., Hannon K.S., Demers L.M., Buchanan J., Bell N.H. Differential effects of gonadal function on bone histomorphometry in male and female rats. J. Bone Min. Res. 4:1989;557-563.
Turner R.T., Wakley G.K., Hannon K.S. Differential effects of androgens on cortical bone histomorphometry in gonadectomized male and female rats. J. Orth. Res. 8:1990;612-617.
Vilmann H. The growth of the cranial base in the albino rat revealed by roentgenocephalometry. J. Zool. 159:1969;283-291.
Wakley G.K., Schritte H.D., Kathleen S.H., Turner R.T. Androgen treatment prevent loss of cancellous bone in the orchidectomized rat. J. Bone Mineral Res. 6:1991;325-330.