Article (Scientific journals)
Toward a standardised protocol for the stable isotope analysis of scleractinian corals
Sturaro, Nicolas; Hsieh, Yunli Eric; Chen, Qi et al.
2020In Rapid Communications in Mass Spectrometry, 34, p. 8663
Peer reviewed
 

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Keywords :
Corals; Stable isotopes
Abstract :
[en] Rationale: The stable isotope analysis of carbon and nitrogen is a powerful tool in many ecological studies, but different sample treatments may affect stable isotope ratios and hamper comparisons among studies. The goal of this study was to determine whether treatments that are commonly used to prepare scleractinian coral samples for stable isotope analysis yield different δ15N and δ13C values, and to provide guidelines toward a standardised protocol. Methods: The animal tissues and Symbiodiniaceae of two symbiotic scleractinian coral species (Stylophora pistillata and Porites lutea) were divided into subsamples to test the effects of the drying method, lipid extraction, acidification treatment and water washing. All of the subsamples were analysed for δ15N and δ13C values, using continuous flow – elemental analysis – isotope ratio mass spectrometry. Results: The drying method and lipid extraction treatment had no substantial effects on the δ15N and δ13C values of Symbiodiniaceae and animal tissues. Acid treatment did cause significant differences in δ13C values (mean differences ≤ 0.5‰, with individual samples becoming up to 2.0‰ more negative), whereas no ecologically significant differences were observed in δ15N values. Animal tissue δ13C values may vary depending on whether samples are washed or not. Conclusions: For going toward a standardised protocol in coral research, we recommend using either available drying method (as they are equally acceptable) for the stable isotope analysis of scleractinian corals, examining the need for lipid extraction on a case-by-case basis, performing a direct acidification of Symbiodiniaceae and animal tissues, and avoiding washing animal tissue with distilled water.
Disciplines :
Aquatic sciences & oceanology
Zoology
Environmental sciences & ecology
Author, co-author :
Sturaro, Nicolas ;  Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanographie biologique
Hsieh, Yunli Eric
Chen, Qi
Wang, Pei-Ling
Denis, Vianney
Language :
English
Title :
Toward a standardised protocol for the stable isotope analysis of scleractinian corals
Publication date :
2020
Journal title :
Rapid Communications in Mass Spectrometry
Volume :
34
Pages :
e8663
Peer reviewed :
Peer reviewed
Available on ORBi :
since 21 January 2021

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