Extracellular traps released by antimicrobial TH17 cells contribute to host defense.

[en] TH17 cell subpopulations have been defined that contribute to inflammation and homeostasis, yet the characteristics of TH17 cells that contribute to host defense against infection are not clear. To elucidate the antimicrobial machinery of the TH17 subset, we studied the response to Cutibacterium acnes, a skin commensal that is resistant to IL-26, the only known TH17 secreted protein with direct antimicrobial activity. We generated C. acnes-specific antimicrobial TH17 clones (AMTH17) with varying antimicrobial activity against C. acnes, which we correlated by RNA-seq to the expression of transcripts encoding proteins that contribute to antimicrobial activity. Additionally, we validated that AMTH17-mediated killing of C. acnes as well as bacterial pathogens, was dependent on the secretion of granulysin, granzyme B, perforin and histone H2B. We found that AMTH17s can release fibrous structures composed of DNA decorated with the histone H2B that entangle C. acnes that we call T cell extracellular traps (TETs). Within acne lesions, H2B and IL-17 colocalized in CD4+ T cells, in proximity to TETs in the extracellular space composed of DNA decorated with H2B. This study identifies a functionally distinct subpopulation of TH17 cells with an ability to form TETs containing secreted antimicrobial proteins that capture and kill bacteria.

Disciplines :

Dermatology
Immunology & infectious disease

Author, co-author :

Agak, George W.

Mouton, Alice ; Université de Liège - ULiège > Département des sciences de la vie > Laboratoire de génétique de la conservation

Teles, Rosane

Weston, Thomas A.

Morselli, Marco

Andrade, Priscila R.

Pellegrini, Matteo

Modlin, Robert L.

Language :

English

Title :

Extracellular traps released by antimicrobial TH17 cells contribute to host defense.

Publication date :

2020

Journal title :

The Journal of clinical investigation

ISSN :

0021-9738

eISSN :

1558-8238

Peer reviewed :

Peer reviewed

Available on ORBi :

since 06 January 2021

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68 (3 by ULiège)

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65 (3 by ULiège)

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