[en] BACKGROUND: During photoaging, the density of melanin chromatophores is heterogeneous in the epidermis. AIMS: To define the patterns of pheomelanin-enriched melanotic hypermelanosis of the face in phototype II subjects and to assess the effect of depigmenting agents. Azelaic acid and glycolic acid were tested as well as a soy extract, reported to reduce pigmentation through interaction with the protease-activated receptor 2 (PAR-2) of keratinocytes. METHOD: Evaluations were made by image analysis of high magnification pictures obtained by a video camera equipped with an internal ultraviolet-emitting unit (Visioscan((R))). RESULTS: Three patterns of subclinical facial hypermelanosis were recognized including the spotty perifollicular type, the accretive globular type and the elongated type of the sunny side of wrinkles. Azelaic acid and the soy extract led to significant skin lightening after a 3-week treatment. By contrast, glycolic acid showed an inconsistent effect. CONCLUSION: Sensitive fluorescence video recording combined with image analysis represents an advance in the noninvasive assessment of the mottled subclinical skin pigmentation. The depigmenting effect observed with the soy extract indicates that the inhibition of PAR-2 may be a novel way to approach certain pigmentary disorders of the skin.
Disciplines :
Dermatology
Author, co-author :
Hermanns, Jean-François ; Université de Liège - ULiège > Département des sciences cliniques > Dermatopathologie
Pierard G.E., Pierard-Franchimont C., Laso Dosal F., Ben Mosbah T., Arrese Estrada J., Rurangirwa A., Dowlati A., Vardar M. (1991) Pigmentary changes in skin senescence. J Appl Cosmetol 9:67-63.
Nikkels A., Ben Mosbah T., Pierard-Franchimont C., De la Brassinne M., Pierard G.E. (1991) Comparative morphometry study of eruptive PUVA-induced and chronic sun-induced lentigines of the skin. Anal Quant Cytol Histol 13:23-26.
Kikuchi I., Idermori M., Uchimura H., Inoue S. (1983) Reflection ultraviolet photography as surface photography of the skin. J Dermatol 10:551-555.
Arai S. (1997) Analysis of pigmentation of human skin (UV-light images)., Wilhelm KP, Elsner P, Berardesca E, Maibach HI (eds): Bioengineering of the Skin: Skin Surface Imaging and Analysis. Boca Raton, CRC Press; 85-94.
Fulton J.E. (1997) Utilizing the ultraviolet (UV detect) camera to enhance the appearance of photo-damage and other skin conditions. Dermatol Surg 23:163-169.
Kollias N., Gillies R., Cohen-Goihman C., Phillips S.B., Muccini J.A., Stiller M., Drake L.A. (1997) Fluorescence photography in the evaluation of hyperpigmentation in photodamaged skin. J Am Acad Dermatol 36:226-230.
Pierard-Franchimont C., Pierard G.E. (1998) Heliodermie heterochrome et risque de cancers cutanes. Rev Med Liege 53:355-356.
Nystedt S., Emilsson K., Wahlestedt C., Sundelin J. (1994) Molecular cloning of a potential proteinase activated receptor. Proc Natl Acad Sci USA 91:9208-9212.
Nystedt S., Larsson A.K., Aberg H., Sundelin J. (1995) The mouse proteinase-activated receptor-2 cDNA and gene: Molecular cloning and functional expression. J Biol Chem 270:5950-5955.
Seiberg M., Paine C., Sharlow E., Andrade-Gordon P., Costanzo M., Eisinger M., Shapiro S. (2000) The protease-activated receptor-2 regulates pigmentation via keratinocyte-melanocyte interactions. Exp Cell Res 254:25-32.
Seiberg M., Paine C., Sharlow E., Andrade-Gordon P., Costanzo M., Eisinger M., Shapiron S. Inhibition of melanosome transfer results in skin lightening. J Invest Dermatol, in press; .
Sharlow E.R., Paine C.S., Babiarz L., Eisinger M., Shapiro S., Seiberg M. The protease activated receptor-2 upregulates keratinocyte phagocytosis. J Invest Dermatol, in press; .
Dery O., Corvera C.U., Steinhoff M., Bunnett N.W. (1998) Proteinase-activated receptors: Novel mechanisms of signaling by serine proteases. Am J Physiol 247.
Pierard G.E. (1998) EEMCO guidance for the assessment of skin colour. J Eur Acad Dermatol Venereol 10:1-11.
Leffell D.J., Stetz M.L., Milstone L.M., Deckelbaum L.I. (1988) In vivo fluorescence of human skin: A potential marker of photoaging. Arch Dermatol 124:1514-1518.
Takema Y., Yorimoto Y., Ohsu H., Osanai O., Kawai M. (1997) Age-related discontinuous changes in the in vivo fluorescence of human facial skin. J Dermatol Sci 15:55-58.
Prota G. (1994) Melanins, melanogenesis and skin photoprotection. Eur J Cancer 30 A:553-554.
Thody A.J., Higgins E.M., Burchill S.A., Marks J.M., Ito S. (1990) Epidermal eumelanin and phaeomelanin concentrations in different skin types and in response to PUVA. Br J Dermatol 122:288-289.
Ortonne J.P., Prota G. (1993) Hair melanins and hair color: Ultrastructural and biochemical aspects. J Invest Dermatol 101.
Dooley T.P. (1997) Topical skin depigmentation agents: Current products and discovery of novel inhibitor of melanogenesis. J Dermatol Treat 8:275-279.
