Scientific conference in universities or research centers (Scientific conferences in universities or research centers)
A novel connection between dicarbonyl stress and epigenetic regulation in triple negative breast cancer
Tiamiou, Assia
2020
 

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Keywords :
Dicarbonyl stress; Epigenetic; Triple-negative breast cancer
Abstract :
[en] Triple-negative breast cancer (TNBC) represents the breast cancer subtype with the worse prognosis and TNBC metabolic profiling indicates that this subtype of breast tumors is generally glycolytic. Methylglyoxal (MG), a very reactive dicarbonyl molecule is derived from glycolysis. In the context of this project we wanted to better understand the relation between MG stress and epigenetic in TNBCs. For that we generated a breast cancer cell line stably depleted for GLO1 (the major defense against MG) to induce an endogenous MG stress. RNA sequencing analysis revealed a pro-metastatic MG signature comprising the regulation of metastatic-related genes expression. Among them, the de novo DNA methyltransferase 3A (DNMT3A) and 3B (DNMT3B) were found significantly increased, suggesting the potential impact of MG stress on epigenetic regulation. Subsequently, we performed a genome wide methylation analysis with 850K CpGs array on GLO1-depleted cells to point to a significant global hypermethylation. The integrative analysis of gene expression data with gene regulatory region methylation status revealed a significant down-regulation of tumor suppressor genes under MG stress in cancer cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Tiamiou, Assia ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire
Language :
English
Title :
A novel connection between dicarbonyl stress and epigenetic regulation in triple negative breast cancer
Alternative titles :
[en] Nouveau lien entre le stress au dicarbonyle et la régulation épigénétique dans le cancer du sein triple négatif
Publication date :
04 December 2020
Event name :
GIGA-Cancer seminar session
Event organizer :
ULiège - Université de Liège
Event place :
Liège, Belgium
Event date :
04 décembre 2020
Available on ORBi :
since 28 December 2020

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