Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species

Tosso, Dji-ndé Félicien; Doucet, Jean-Louis; Kaymak, Esra; Daïnou, Kasso; Duminil, Jerome; Hardy, Olivier

doi:10.3732/apps.1600029

Download

Article (Scientific journals)

Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species

Tosso, Dji-ndé Félicien; Doucet, Jean-Louis; Kaymak, Esra et al.

2016 • In Applications in Plant Sciences, 4

Peer Reviewed verified by ORBi

Permalink
https://hdl.handle.net/2268/254167

DOI
10.3732/apps.1600029

Files (1)Send to Details Statistics Bibliography Similar publications

Files

Full Text

Tosso et al. 2016_nSSRs Guibourtia tessmannii.pdf

Publisher postprint (544.21 kB)

Download

All documents in ORBi are protected by a user license.

Send to

RIS BibTex APA Chicago Permalink X Linkedin

Details

Keywords :

Fabaceae; flow cytometry;; Guibourtia; microsatellites; next-generation sequencing; olyploidy

Abstract :

[en] Premise of the study: Nuclear microsatellites (nSSRs) were designed for Guibourtia tessmannii (Fabaceae, Caesalpinioideae), a highly exploited African timber tree, to study population genetic structure and gene flow. • Methods and Results: We developed 16 polymorphic nSSRs from a genomic library tested in three populations of G. tessmannii and two populations of G. coleosperma. These nSSRs display three to 14 alleles per locus (mean 8.94) in G. tessmannii. Crossamplification tests in nine congeneric species demonstrated that the genus Guibourtia contains diploid and polyploid species. Flow cytometry results combined with nSSR profiles suggest that G. tessmannii is octoploid. • Conclusions: nSSRs revealed that African Guibourtia species include both diploid and polyploid species. These markers will provide information on the mating system, patterns of gene flow, and genetic structure of African Guibourtia species.

Disciplines :

Genetics & genetic processes

Author, co-author :

Tosso, Dji-ndé Félicien ; Université de Liège > Ingénierie des biosystèmes (Biose) > Laboratoire de Foresterie des régions trop. et subtropicales

Doucet, Jean-Louis ; Université de Liège > Ingénierie des biosystèmes (Biose) > Laboratoire de Foresterie des régions trop. et subtropicales

Kaymak, Esra

Daïnou, Kasso ; Université de Liège > Ingénierie des biosystèmes (Biose) > Laboratoire de Foresterie des régions trop. et subtropicales

Duminil, Jerome

Hardy, Olivier

Language :

English

Title :

Microsatellite development for the genus Guibourtia (Fabaceae, Caesalpinioideae) reveals diploid and polyploid species

Publication date :

July 2016

Journal title :

Applications in Plant Sciences

eISSN :

2168-0450

Publisher :

Botanical Society of America, St. Louis, United States - Missouri

Volume :

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

Etude génétique et physiologiques du genre Guibourtia dans un contexte de changement climatique

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique

Available on ORBi :

since 20 December 2020

Statistics

Number of views

65 (1 by ULiège)

Number of downloads

49 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Chybicki, I. J., and J. Burczyk. 2009. Simultaneous estimation of null alleles and inbreeding coefficients. Journal of Heredity 100: 106-113.
Culley, T. M., S. G. Weller, A. K. Sakai, and K. A. Putnam. 2008. Characterization of microsatellite loci in the Hawaiian endemic shrub Schiedea adamantis (Caryophyllaceae) and amplification in related species and genera. Molecular Ecology Resources 8: 1081-1084.
Fougère-Danezan, M., S. Maumont, and A. Bruneau. 2007. Relationships among resin-producing Detarieae s.l. (Leguminosae) as inferred by molecular data. Systematic Botany 32: 748-761.
Fu, X., Y. Huang, S. Deng, R. Zhou, G. Yang, X. Ni, W. Li, and S. Shi. 2005. Construction of a SSH library of Aegiceras corniculatum under salt stress and expression analysis of four transcripts. Plant Science 169: 147-154.
Hardy, O. J., and X. Vekemans. 2002. SPAGeDi: A versatile computer program to analyse spatial genetic structure at the individual or population levels. Molecular Ecology Notes 2: 618-620.
Holleley, C. E., and P. G. Geerts. 2009. Multiplex Manager 1.0: A crossplatform computer program that plans and optimizes multiplex PCR. BioTechniques 46: 511-517.
Léonard, J. 1949. Notulae systematicae IV (Caesalpiniaceae-Amherstieae africanae americanaeque). Bulletin du Jardin Botanique de l'État, Bruxelles/Bulletin van den Rijksplantentuin, Brussel 19: 383-408.
Mariac, C., N. Scarcelli, J. Pouzadou, A. Barnaud, C. Billot, A. Faye, A. Kougbeadjo, et al. 2014. Cost-effective enrichment hybridization capture of chloroplast genomes at deep multiplexing levels for population genetics and phylogeography studies. Molecular Ecology Resources 14: 1103-1113.
Marie, D., and S. C. Brown . 1993. A cytometric exercise in plant DNA histograms, with 2C values for 70 species. Biology of the Cell 78: 41-51.
Masella, A. P., A. K. Bartram, J. M. Truszkowski, D. G. Brown, and J. D. Neufeld. 2012. PANDAseq: Paired-end assembler for Illumina sequences. BMC Bioinformatics 13: 31.
Meglécz, E., N. Pech, A. Gilles, V. Dubut, P. Hing amp, A. Trilles, R. Grenier, and J. F. Martin. 2014. QDD version 3.1: A user-friendly computer program for microsatellite selection and primer design revisited: Experimental validation of variables determining genotyping success rate. Molecular Ecology Resources 14: 1302-1313.
Micheneau, C., G. Dauby, N. Bourland, J.-L. Doucet, and O. J. Hardy. 2011. Development and characterization of microsatellite loci in Pericopsis elata (Fabaceae) using a cost-efficient approach. American Journal of Botany 98: E268-e270.
Schuelke, M. 2000. An economic method for the fluorescent labeling of PCR fragments. Nature Biotechnology 18: 233-234.
Tosso, F., K. Daïnou, O. J. Hardy, B. Sinsin, and J.-L. Doucet. 2015. Le genre Guibourtia Benn., un taxon a haute valeur commerciale et societale (synthese bibliographique). Biotechnologie, Agronomie, Société et Environnement 19: 71-88.