Caractérisation moléculaire du virus d'Epstein-Barr associé au lymphome de Hodgkin classique en Tunisie: Prévalence des délétions de l'oncogène LMP1 et des souches virales A et B
Trimeche, M.; Korbi, S.; Ziadi, S.et al.
2005 • In Annales de Biologie Clinique, 63 (2, MAR-APR), p. 193-199
[en] Epstein-Barr virus (EBV) is detected in Hodgkin's lymphoma (HL) at variable frequencies, depending on various factors including the geographic location. The prevalence of EBV is high among HL diagnosed in the region of the center of Tunisia. The aim of the current work was to define the genotypic features of EBV in a series of EBV-positive HL in this area, in comparison to those of EBV found in association with benign lymphadenopathies. A 30 bp and a 69 bp deletion within the LMP1 gene were detected in 41% and 11% of HL cases, respectively. In reactive lymph nodes, the 30 bp deletion was found in 43% of the cases. Type A EBV was detected in 84% of HL cases and in 96% of reactive lymphoadenopathies, and type B EBV was detected in others. No significant association was found between histological type of HL, age of the patients, or stage at diagnosis, and the molecular configuration of LMP1. However, LMP1 deletions were found more frequently among male patients. The significance of these results is discussed.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Trimeche, M.
Korbi, S.
Ziadi, S.
Amara, K.
Boniver, Jacques ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
de Leval, Laurence ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Language :
French
Title :
Caractérisation moléculaire du virus d'Epstein-Barr associé au lymphome de Hodgkin classique en Tunisie: Prévalence des délétions de l'oncogène LMP1 et des souches virales A et B
Alternative titles :
[en] Molecular characterization of Epstein-Barr virus associated with classical Hodgkin's lymphoma in Tunisia: prevalence of the LMP1 oncegene deletions and A and B viruses strains
IARC working group on the evaluation of carcinogenic risks to humans. Epstein-Barr virus and Kaposi's sarcoma herpesvirus/human herpesvirus 8. Lyon : IARC Press, 1997.
Glaser SL, Lin RJ, Stewart SL, et al. Epstein-Barr virus-associated Hodgkin's disease : epidemiologic characteristics in international data. Int J Cancer 1997 ; 70 : 375-82.
Delsol G, Brousset P, Chittal S, Rigal-Huguet F. Correlation of the expression of Epstein-Barr virus-latent membrane protein and in situ hybridization with biotinylated Bam HI-W probes in Hodgkin's disease. Am J Pathol 1992 ; 140 : 247-53.
Wang D, Liebowitz D, Kieff E. An EBV membrane protein expressed in immortalized lymphocytes transforms established rodent cells. Cell 1985 ; 43 : 831-40.
Hu LF, Zabarovsky ER, Chen F, et al. Isolation and sequencing of the Epstein-Barr virus BNLF-1 gene (LMP-1) from a Chinese nasopharyngeal carcinoma. J Gen Virol 1991 ; 72 : 2399-409.
Chen ML, Tsai CN, Liang CL, et al. Cloning and characterization of the latent membrane protein (LMP) of a specific Epstein-Barr virus variant derived from the nasopharyngeal carcinoma in the Taiwanese population. Oncogene 1992 ; 7 : 2131-40.
Leung SY, Yuen ST, Chung LP, Chan AS, Wong MP. Prevalence of mutations and 30-bp deletion in the C-terminal region of Epstein-Barr virus latent membrane protein-1 oncogene in reactive lymphoid tissue and non-nasopharyngeal EBV-associated carcinomas in Hong Kong Chinese. Int J Cancer 1997 ; 72 : 225-30.
Knecht H, Bachmann E, Brousset P, et al. Deletions within the LMP-1 oncogene of Epstein-Barr virus are clustered in Hodgkin's disease and identical to those observed in nasopharyngeal carcinoma. Blood 1993 ; 82 : 2937-42.
Sandvej K, Peh SC, Andresen BS, Pallesen G. Identification of potential hot spots in the carboxy-terminal part of the Epstein-Barr virus (EBV) BNLF-1 gene in both malignant and benign EBV-associated diseases : high frequency of a 30-bp deletion in Malaysian and Danish peripheral T-cell lymphomas. Blood 1994 ; 84 : 4053-60.
Knecht H, Raphael M, McQuain C, et al. Deletion variants within the NF-kB activation domain of the LMP1 oncogene prevail in acquired immunodeficiency syndrome-related large cell lymphomas and human immunodeficiency virus-negative atypical lymphoproliferations. Blood 1996 ; 87 : 876-81.
Knecht H, Martius F, Bachmann E, et al. A deletion mutant of the LMP-1 oncogene of Epstein-Barr virus is associated with evolution of angioimmunoblastic lymphadenopathy into B immunoblastic lymphoma. Leukemia 1995 ; 9 : 458-65.
Chang YS, Su IJ, Chung PJ, et al. Detection of an Epstein-Barr virus variant in T-cell lymphoma tissue identical to the distinct strain observed in nasopharyngeal carcinoma in the Taiwanaise population. Int J Cancer 1995 ; 62 : 673-7.
Chen WG, Chen YY, Bacchi MM, Bacchi CE, Alvarenga M, Weiss LM. Genotyping of Epstein-Barr virus in Brazilian Burkitt's lymphoma and reactive lymphoid tissue : type A with a high prevalence of deletions within the latent membrane protein gene. Am J Pathol 1996 ; 148 : 17-23.
Kingma DW, Weiss WB, Jaffe ES, Kumar S, Frekko K, Raffeld M. Epstein-Barr virus latent membrane protein-1 oncogene deletions : correlations with malignancy in Epstein-Barr virus-associated lymphoproliferative disorders and malignant lymphomas. Blood 1996 ; 88 : 242-51.
Santon A, Martin C, Manzanal AI, Preciado MV, Bellas C. Paediatric Hodgkin's disease in Spain : association with Epstein-Barr virus strains carrying latent membrane protein-1 oncogene deletions and high frequency of dual infections. Br J Haematol 1998 ; 103 : 129-36.
Klein C, Rothenberger S, Niemeyer C, et al. EBV-associated lymphoproliferative syndrome with a distinct 69 base-pair deletion in the LMP-1 oncogene. Br J Haematol 1995 ; 91 : 938-40.
Tacyildiz N, Cavdar AO, Ertem U, et al. Unusually high frequency of a 69-bp deletion within the carboxy terminus of the LMP1 oncogene of Epstein-Barr virus detected in Burkitt's lymphoma of Turkish children. Leukemia 1998 ; 12 : 1796-805.
Dirnhofer S, Angeles-Angeles A, Ortiz-Hidalgo C, et al. High prevalence of a 30-base pair deletion in the Epstein-Barr virus (EBV) latent membrane protein 1 gene and of strain type B EBV in Mexican classical Hodgkin's disease and reactive lymphoid tissue. Hum Pathol 1999 ; 30 : 781-7.
Kim I, Park ER, Park SH, Lin Z, Kim YS. Characteristics of Epstein-Barr virus isolated from the malignant lymphomas in Korea. J Med Virol 2002 ; 67 : 59-66.
Hu LF, Chen F, Zheng X, et al. Clonability and tumorigenicity of human epithelial cells expressing the EBV encoded membrane protein LMP1. Oncogene 1993 ; 8 : 1575-83.
Li SN, Chang YS, Liu ST. Effect of a 10-amino acid deletion on the oncogenic activity of latent membrane protein 1 of Epstein-Barr virus. Oncogene 1996 ; 12 : 2129-35.
Dolcetti R, Quaia M, Gloghini A, et al. Biologically relevant phenotypic changes and enhanced growth properties induced in B lymphocytes by an EBV strain derived from a histologically aggressive Hodgkin's disease. Int J Cancer 1999 ; 80 : 240-9.
Khanim F, Yao QY, Niedobitek G, Sihota S, Rickinson AB, Young LS. Analysis of Epstein-Barr virus gene polymorphisms in normal donors and in virus-associated tumours from different geographic locations. Blood 1996 ; 88 : 491-501.
Hayashi K, Chen WG, Chen YY, et al. Deletion of Epstein-Barr virus latent membrane protein-1 gene in United States and Brazilian Hodgkin's disease and reactive lymphoid tissue : high frequency of a 30-bp deletion. Hum Pathol 1997 ; 28 : 1408-14.
Hayashi K, Chen WG, Chen YY, et al. Deletion of Epstein-Barr virus latent membrane protein 1 gene in Japanese and Brazilian gastric carcinomas, metastatic lesions, and reactive lymphocytes. Am J Pathol 1998 ; 152 : 191-8.
Itakura O, Yamada S, Narita M, Kikuta H. High prevalence of a 30-base pair deletion within the carboxy terminal end of the LMP-1 oncogene of Epstein-Barr virus in the Japanese population. Oncogene 1996 ; 13 : 1549-53.
Mansoor A, Stevenson MS, Li RZ, et al. Prevalence of Epstein-Barr viral sequences and EBV LMP1 oncogene deletions in Burkitt's lymphoma from Pakistan : epidemiological correlations. Hum Pathol 1997 ; 28 : 283-8.
Cheinfeld AG, Nador RG, Cesarman E, Chadburn A, Knowles DM. Epstein-Barr virus latent membrane protein-1 oncogene deletion in post-transplantation lymphoproliferative disorders. Am J Pathol 1997 ; 151 : 805-12.
Smir BN, Hauke RJ, Bierman PJ, et al. Molecular epidemiology of deletions and mutations of the latent membrane protein 1 oncogene of the Epstein-Barr virus in posttransplant lymphoproliferative disorders. Lab Invest 1996 ; 75 : 575-88.
Adldinger HK, Delius H, Freese UK, Clarke J, Bornkamm GW. A putative transforming gene of Jijoye virus differs from that of Epstein-Barr virus prototypes. Virology 1985 ; 141 : 221-34.
Rowe M, Young LS, Cadwallader K, Petti L, Kieff E, Rickinson AB. Distinction between Epstein-Barr virus type A (EBNA 2A) and type B (EBNA 2B) isolates extends to the EBNA 3 family of nuclear proteins. J Virol 1989 ; 63 : 1031-9.
Rickinson AB, Young LS, Rowe M. Influence of the Epstein-Barr virus nuclear antigen EBNA 2 on the growth phenotype of virus-transformed B cells. J Virol 1987 ; 61 : 1310-7.
Young LS, Yao QY, Rooney CM, et al. New type B isolates of Epstein-Barr virus from Burkitt's lymphoma and from normal individuals in endemic areas. J Gen Virol 1987 ; 68 : 2853-62.
Boyle MJ, Vasak E, Tschuchnigg M, et al. Subtypes of Epstein-Barr virus (EBV) in Hodgkin's disease : association between B-type EBV and immunocompromise. Blood 1993 ; 81 : 468-74.
Boyle MJ, Sewell WA, Sculley TB, et al. Subtypes of Epstein-Barr virus in human immunodeficiency virus-associated non-Hodgkin lymphoma. Blood 1991 ; 78 : 3004-11.
Belkaid ML, Briere J, Djebbara Z, Beldjord K, Andrieu JM, Colonna P. Comparison of Epstein-Barr virus markers in Reed-sternberg cells in adult Hodgkin's disease tissues from an industrialized and a developing country. Leuk Lymphoma 1995 ; 17 : 163-8.
Korbi S, Trimeche M, Sriha B, et al. Epstein-Barr virus in Hodgkin's disease : the example of central Tunisia. Ann Pathol 2002 ; 22 : 96-101.
Edinger JW, Bonneville M, Scotet E, Houssaint E, Schumacher HR, Posnett DN. EBV gene expression not altered in rheumatoid synovia despite the presence of EBV antigen-specific T cell clones. J Immunol 1999 ; 162 : 3694-701.
Lin JC, Lin SC, De BK, Chan WP, Evatt B. Precision of genotyping of Epstein-Barr virus by polymerase chain reaction using three gene loci (EBNA-2, EBNA-3C, and EBER) : predominance of type A virus associated with Hodgkin's disease. Blood 1993 ; 81 : 3372-81.
Essop MF, Engel M, Close P, Sinclair-Smith C, Pallesen G. Epstein-barr virus in Hodgkin's disease : frequency of a 30-bp deletion in the latent membrane protein (LMP-1) oncogene in South African patients. Int J Cancer 1999 ; 84 : 449-51.
Zhou XG, Sandvej K, Li PJ, et al. Epstein-Barr virus gene polymorphisms in Chinese Hodgkin's disease cases and healthy donors : identification of three distinct virus variants. J Gen Virol 2001 ; 82 : 1157-67.
Dolcetti R, Zancai P, De Re V, et al. Epstein-Barr virus strains with latent membrane protein-1 deletions : Prevalence in the Italian population and high association with human immunodeficiency virus-related Hodgkin's disease. Blood 1997 ; 89 : 1723-31.
Kingma DW, Weiss WB, Jaffee ES, Kumar S, Frekko K, Raffeld M. Epstein-Barr virus latent membrane protein-1 oncogene deletions : correlations with malignancy in Epstein-Barr virus-associated lymphoproliferative disorders and malignant lymphomas. Blood 1996 ; 88 : 242-51.
Kaye KM, Izumi KM, Mosialos G, Kieff E. The Epstein-Barr virus LMP1 cytoplasmic carboxy terminus is essential for B-lymphocyte transformation ; fibroblast cocultivation complements a critical function within the terminal 155 residues. J Virol 1995 ; 69 : 675-83.
Mitchell T, Sugden B. Stimulation of NF-kappa B-mediated transcription by mutant derivatives of the latent membrane protein of Epstein-Barr virus. J Virol 1995 ; 69 : 2968-76.
Mosialos G, Birkenbach M, Yalamanchili R, Vanarsdale T, Ware C, Kieff E. The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family. Cell 1995 ; 80 : 389-99.
Chiang AK, Wong KY, Liang AC, Srivastava G. Comparative analysis of Epstein-Barr virus gene polymorphisms in nasal T/NK-cell lymphomas and normal nasal tissues : implication on virus strain selection in malignancy. Int J Cancer 1999 ; 80 : 356-64.
Sandvej K, Gratama JW, Munch M, et al. Sequence analysis of the Epstein-Barr virus (EBV) latent membrane protein-1 gene and promoter region : identification of four variants among wild-type EBV isolates. Blood 1997 ; 90 : 323-30.
Krugmann J, Tzankov A, Gschwendtner A, et al. Longer failure-free survival interval of Epstein-Barr virus-associated classical Hodgkin's lymphoma : a single-institution study. Mod Pathol 2003 ; 16 : 566-73.
Bellas C, Santon A, Manzanal A, et al. Pathological, immunological, and molecular features of Hodgkin's disease association with HIV infection. Comparison with ordinary Hodgkin's disease. Am J Surg Pathol 1996 ; 20 : 1520-4.
Tomita Y, Ohsawa M, Kanno H, et al. Epstein-Barr virus in Hodgkin's disease patients in Japan. Cancer 1996 ; 77 : 186-92.
