[en] To meet increasing global food demand, breeders and scientists aim to improve the yield and quality of major food crops. Plant diseases threaten food security and are expected to increase because of climate change. CRISPR genome-editing technology opens new opportunities to engineer disease resistance traits. With precise genome engineering and transgene-free applications, CRISPR is expected to resolve the major challenges to crop improvement. Here, we discuss the latest developments in CRISPR technologies for engineering resistance to viruses, bacteria, fungi, and pests. We conclude by highlighting current concerns and gaps in technology, as well as outstanding questions for future research.
Hickey, L.T., Breeding crops to feed 10 billion (2019) Nat Biotechnol, 37 (7), pp. 744-754. , COI: 1:CAS:528:DC%2BC1MXhtFymtbfM, PID: 31209375
Ray, D.K., Recent patterns of crop yield growth and stagnation (2012) Nat Commun, 3, p. 1293. , PID: 23250423, COI: 1:CAS:528:DC%2BC3sXhsVCjsro%3D
Ritchie, H.M., Roseruse, L., (2019), https://ourworldindata.org/land-use, Published online at OurWorldInData.org, Accessed 22 Nov 2020
Añel, J.A., Understanding climate change from a global analysis of city analogues (2019) Plos One, 14 (7). , COI: 1:CAS:528:DC%2BC1MXhs12ktL7P
Newbery, F., Qi, A., Fitt, B.D., Modelling impacts of climate change on arable crop diseases: progress, challenges and applications (2016) Curr Opin Plant Biol, 32, pp. 101-109. , PID: 27471781
Roussi, A., Why gigantic locust swarms are challenging governments and researchers (2020) Nature, 579 (7799), p. 330. , COI: 1:CAS:528:DC%2BB3cXltFCit7g%3D, PID: 32184472
Pingali, P.L., Green revolution: impacts, limits, and the path ahead (2012) Proc Natl Acad Sci U S A, 109 (31), pp. 12302-12308. , COI: 1:CAS:528:DC%2BC38XhtleqsrvJ, PID: 22826253
Tester, M., Langridge, P., Breeding technologies to increase crop production in a changing world (2010) Science, 327 (5967), pp. 818-822. , COI: 1:CAS:528:DC%2BC3cXhslWisLg%3D, PID: 20150489
Bailey-Serres, J., Genetic strategies for improving crop yields (2019) Nature, 575 (7781), pp. 109-118. , COI: 1:CAS:528:DC%2BC1MXitFWnurrE, PID: 31695205
Ezezika, O.C., Factors influencing agbiotech adoption and development in sub-Saharan Africa (2012) Nat Biotechnol, 30 (1), pp. 38-40. , COI: 1:CAS:528:DC%2BC38XlsF2nug%3D%3D, PID: 22231092
Zaidi, S.S., New plant breeding technologies for food security (2019) Science, 363 (6434), pp. 1390-1391. , COI: 1:CAS:528:DC%2BC1MXhtFCjurfO, PID: 30923209
Ghosh, S., Speed breeding in growth chambers and glasshouses for crop breeding and model plant research (2018) Nat Protoc, 13 (12), pp. 2944-2963. , COI: 1:CAS:528:DC%2BC1cXit1eiu7rE, PID: 30446746
Watson, A., Speed breeding is a powerful tool to accelerate crop research and breeding (2018) Nat Plants, 4 (1), pp. 23-29. , PID: 29292376
Barabaschi, D., Next generation breeding (2016) Plant Sci, 242, pp. 3-13. , COI: 1:CAS:528:DC%2BC2MXhtlSjur7M, PID: 26566820
Zorrilla-Fontanesi, Y., Strategies to revise agrosystems and breeding for Fusarium 1 wilt control of banana (2020) Nature Foods, , In Press
He, Y., Zhao, Y., Technological breakthroughs in generating transgene-free and genetically stable CRISPR-edited plants (2019) aBIOTECH, 1 (1), pp. 88-96
Liang, Z., Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins (2018) Nat Protoc, 13, pp. 413-430. , COI: 1:CAS:528:DC%2BC1cXitVCmsrc%3D, PID: 29388938
Woo, J.W., DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins (2015) Nat Biotech, 33 (11), pp. 1162-1164. , COI: 1:CAS:528:DC%2BC2MXhs1yjtrnL
Baltes, N.J., DNA replicons for plant genome engineering (2014) Plant Cell, 26 (1), pp. 151-163. , COI: 1:CAS:528:DC%2BC2cXks1Sguro%3D, PID: 24443519
Demirer, G.S., High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants (2019) Nat Nanotechnol, 14 (5), pp. 456-464. , COI: 1:CAS:528:DC%2BC1MXmtlynt78%3D, PID: 30804481
Kwak, S.Y., Chloroplast-selective gene delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers (2019) Nat Nanotechnol, 14 (5), pp. 447-455. , COI: 1:CAS:528:DC%2BC1MXmtlyntrY%3D, PID: 30804482
Waltz, E., Gene-edited CRISPR mushroom escapes US regulation (2016) Nature, 532 (7599), p. 293. , COI: 1:CAS:528:DC%2BC28XmsFaqsrg%3D, PID: 27111611
Waltz, E., CRISPR-edited crops free to enter market, skip regulation (2016) Nat Biotechnol, 34 (6), p. 582. , COI: 1:CAS:528:DC%2BC28XpsFClt7w%3D, PID: 27281401
Waltz, E., With a free pass, CRISPR-edited plants reach market in record time (2018) Nat Biotechnol, 36 (1), pp. 6-7. , COI: 1:CAS:528:DC%2BC1cXmtlSksw%3D%3D, PID: 29319694
Pickar-Oliver, A., Gersbach, C.A., The next generation of CRISPR–Cas technologies and applications (2019) Nat Rev Mol Cell Biol, 20 (8), pp. 490-507. , COI: 1:CAS:528:DC%2BC1MXhtVOju7jM, PID: 31147612
Stella, S., Montoya, G., The genome editing revolution: a CRISPR-Cas TALE off-target story (2016) Bioessays, 38 (S1), pp. S4-S13. , COI: 1:CAS:528:DC%2BC28XhtFKksrnJ, PID: 27417121
Chen, K., CRISPR/Cas genome editing and precision plant breeding in agriculture (2019) Annu Rev Plant Biol, 70, pp. 667-697. , COI: 1:CAS:528:DC%2BC1MXktFegsr8%3D, PID: 30835493
Li, J.F., Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9 (2013) Nat Biotechnol, 31 (8), pp. 688-691. , COI: 1:CAS:528:DC%2BC3sXht1Cgs7jM, PID: 23929339
Shan, Q., Targeted genome modification of crop plants using a CRISPR-Cas system (2013) Nat Biotechnol, 31 (8), pp. 686-688. , COI: 1:CAS:528:DC%2BC3sXht1Cgsb%2FI, PID: 23929338
Nekrasov, V., Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease (2013) Nat Biotechnol, 31 (8), pp. 691-693. , COI: 1:CAS:528:DC%2BC3sXht1CgtrnP, PID: 23929340
Hanna, R.E., Doench, J.G., Design and analysis of CRISPR-Cas experiments (2020) Nat Biotechnol, 38 (7), pp. 813-823. , COI: 1:CAS:528:DC%2BB3cXmvFylu7c%3D, PID: 32284587
Jinek, M., A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity (2012) Science, 337 (6096), pp. 816-821. , COI: 1:CAS:528:DC%2BC38XhtFOqsb3L, PID: 22745249
Wright, A.V., Nunez, J.K., Doudna, J.A., Biology and applications of CRISPR systems: harnessing nature’s toolbox for genome engineering (2016) Cell, 164 (1-2), pp. 29-44. , COI: 1:CAS:528:DC%2BC28XhtFShsbw%3D, PID: 26771484
O'Connell, M.R., Programmable RNA recognition and cleavage by CRISPR/Cas9 (2014) Nature, 516 (7530), pp. 263-266. , COI: 1:CAS:528:DC%2BC2cXhvVemtr%2FK, PID: 25274302
Zaidi, S.S., Mahfouz, M.M., Mansoor, S., CRISPR-Cpf1: a new tool for plant genome editing (2017) Trends Plant Sci, 22 (7), pp. 550-553. , COI: 1:CAS:528:DC%2BC2sXnsl2qsrw%3D, PID: 28532598
Aman, R., RNA virus interference via CRISPR/Cas13a system in plants (2018) Genome Biol, 19 (1), p. 1. , PID: 29301551, COI: 1:CAS:528:DC%2BC1cXitFGrsbfN
Abudayyeh, O.O., RNA targeting with CRISPR–Cas13 (2017) Nature, 550, pp. 280-284
Smargon, A.A., Cas13b is a type VI-B CRISPR-associated RNA-guided RNase differentially regulated by accessory proteins Csx27 and Csx28 (2017) Mol Cell, 65 (4), pp. 618-630. , COI: 1:CAS:528:DC%2BC2sXltlSlug%3D%3D, PID: 28065598, e7
Guilinger, J.P., Thompson, D.B., Liu, D.R., Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification (2014) Nat Biotechnol, 32 (6), pp. 577-582. , COI: 1:CAS:528:DC%2BC2cXmvV2ktL4%3D, PID: 24770324
Li, Z., A potent Cas9-derived gene activator for plant and mammalian cells (2017) Nat Plants, 3, pp. 930-936
Piatek, A., RNA-guided transcriptional regulation in planta via synthetic dCas9-based transcription factors (2015) Plant Biotechnol J, 13 (4), pp. 578-589. , COI: 1:CAS:528:DC%2BC2MXntFSgtbg%3D, PID: 25400128
Terns, M.P., CRISPR-based technologies: impact of RNA-targeting systems (2018) Mol Cell, 72 (3), pp. 404-412. , COI: 1:CAS:528:DC%2BC1cXitVyhurzK, PID: 30388409
Shimatani, Z., Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion (2017) Nat Biotechnol, 35, pp. 441-443
Xu, Z., Engineering broad-spectrum bacterial blight resistance by simultaneously disrupting variable TALE-binding elements of multiple susceptibility genes in rice (2019) Mol Plant, 12 (11), pp. 1434-1446. , COI: 1:CAS:528:DC%2BC1MXitVKgt73P, PID: 31493565
Oliva, R., Broad-spectrum resistance to bacterial blight in rice using genome editing (2019) Nat Biotechnol, 37 (11), pp. 1344-1350. , COI: 1:CAS:528:DC%2BC1MXitVCksL3F, PID: 31659337
Si, X., Manipulating gene translation in plants by CRISPR-Cas9-mediated genome editing of upstream open reading frames (2020) Nat Protoc, 15 (2), pp. 338-363. , COI: 1:CAS:528:DC%2BB3cXlslaqsw%3D%3D, PID: 31915386
Cai, Y., CRISPR/Cas9-mediated deletion of large genomic fragments in soybean (2018) Int J Mol Sci, 19 (12), p. 3835. , COI: 1:CAS:528:DC%2BC1MXot1Wlsbs%3D
Zhou, H., Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice (2014) Nucleic Acids Res, 42 (17), pp. 10903-10914. , COI: 1:CAS:528:DC%2BC2MXis1Chtbk%3D, PID: 25200087
Nishimasu, H., Engineered CRISPR-Cas9 nuclease with expanded targeting space (2018) Science, 361 (6408), pp. 1259-1262. , COI: 1:CAS:528:DC%2BC1cXhsleht7jJ, PID: 30166441
Ashfield, T., Evolution of a complex disease resistance gene cluster in diploid Phaseolus and tetraploid Glycine (2012) Plant Physiol, 159 (1), pp. 336-354. , COI: 1:CAS:528:DC%2BC38XntV2gs7c%3D, PID: 22457424
van Vu, T., Challenges and perspectives in homology-directed gene targeting in monocot plants (2019) Rice, 12, p. 95
Liu, M., Methodologies for improving HDR efficiency (2019) Front Genet, 9, p. 691
Butt, H., Efficient CRISPR/Cas9-mediated genome editing using a chimeric single-guide RNA molecule (2017) Front Plant Sci, 8, p. 1441. , PID: 28883826
Wang, M., Gene targeting by homology-directed repair in rice using a geminivirus-based CRISPR/Cas9 system (2017) Mol Plant, 10 (7), pp. 1007-1010. , COI: 1:CAS:528:DC%2BC2sXls1Wiur8%3D, PID: 28315751
Zhang, H., Back into the wild-apply untapped genetic diversity of wild relatives for crop improvement (2017) Evol Appl, 10 (1), pp. 5-24. , PID: 28035232
Arora, S., Resistance gene cloning from a wild crop relative by sequence capture and association genetics (2019) Nat Biotechnol, 37 (2), pp. 139-143. , COI: 1:CAS:528:DC%2BC1MXosV2qtrw%3D, PID: 30718880
Kourelis, J., van der Hoorn, R.A.L., Defended to the nines: 25 years of resistance gene cloning identifies nine mechanisms for R protein function (2018) Plant Cell, 30 (2), pp. 285-299
Komor, A.C., Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage (2016) Nature, 533 (7603), pp. 420-424. , COI: 1:CAS:528:DC%2BC28XmsVehsr8%3D, PID: 27096365
Bastet, A., Mimicking natural polymorphism in eIF4E by CRISPR-Cas9 base editing is associated with resistance to potyviruses (2019) Plant Biotechnol J, 17 (9), pp. 1736-1750. , COI: 1:CAS:528:DC%2BC1MXhsFOjtLrL, PID: 30784179
Butt, H., CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors (2019) Genome Biol, 20 (1), p. 73. , PID: 31036069
Li, C., Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors (2020) Nat Biotechnol, 38 (7), pp. 875-882. , COI: 1:CAS:528:DC%2BB3cXotFGltQ%3D%3D, PID: 31932727
Butt, H., CRISPR-based directed evolution for crop improvement (2020) Trends Biotechnol, 38 (3), pp. 236-240. , COI: 1:CAS:528:DC%2BC1MXhs1alurvI, PID: 31477243
Aman, R., Engineering RNA virus interference via the CRISPR/Cas13 machinery in Arabidopsis (2018) Viruses, 10 (12), p. 732
Zhang, T., Establishing CRISPR/Cas13a immune system conferring RNA virus resistance in both dicot and monocot plants (2019) Plant Biotechnol J, 17 (7), pp. 1185-1187. , PID: 30785668
Zhang, T., Establishing RNA virus resistance in plants by harnessing CRISPR immune system (2018) Plant Biotechnol J, 16 (8), pp. 1415-1423. , COI: 1:CAS:528:DC%2BC1cXhtlSlsL%2FL, PID: 29327438
Ali, Z., CRISPR/Cas9-mediated viral interference in plants (2015) Genome Biol, 16 (1), p. 238. , PID: 26556628, COI: 1:CAS:528:DC%2BC28Xotlajtr8%3D
Ali, Z., CRISPR/Cas9-mediated immunity to geminiviruses: differential interference and evasion (2016) Sci Rep, 6, p. 26912. , COI: 1:CAS:528:DC%2BC28XovVyrtb4%3D, PID: 27225592
Baltes, N.J., Conferring resistance to geminiviruses with the CRISPR–Cas prokaryotic immune system (2015) Nat Plants, 1 (10), p. 15145. , COI: 1:CAS:528:DC%2BC2MXhvFClsLfK
Ji, X., Establishing a CRISPR-Cas-like immune system conferring DNA virus resistance in plants (2015) Nat Plants, 1 (10), p. 15144. , COI: 1:CAS:528:DC%2BC2MXhvFClsLfJ, PID: 27251395
Tashkandi, M., Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato (2018) Plant Signal Behav, 13 (10). , PID: 30289378, COI: 1:CAS:528:DC%2BC1cXhvVGms7fO
Yin, K., Engineer complete resistance to Cotton Leaf Curl Multan virus by the CRISPR/Cas9 system in Nicotiana benthamiana (2019) Phytopathol Res, 1
Mehta, D., Linking CRISPR-Cas9 interference in cassava to the evolution of editing-resistant geminiviruses (2019) Genome Biol, 20 (1), p. 80. , PID: 31018865
Roy, A., Multiplexed editing of a begomovirus genome restricts escape mutant formation and disease development (2019) Plos One, 14 (10). , COI: 1:CAS:528:DC%2BC1MXit1Witb7K, PID: 31644604
Tripathi, J.N., CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding (2019) Commun Biol, 2, p. 46. , PID: 30729184
Macovei, A., Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus (2018) Plant Biotechnol J, 16 (11), pp. 1918-1927. , COI: 1:CAS:528:DC%2BC1cXhvFSjurnI, PID: 29604159
Gomez, M.A., Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence (2019) Plant Biotechnol J, 17 (2), pp. 421-434. , COI: 1:CAS:528:DC%2BC1MXhtFCgtrY%3D, PID: 30019807
Santillan Martinez, M.I., CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew (2020) BMC Plant Biol, 20 (1), p. 284. , COI: 1:CAS:528:DC%2BB3cXht1Cmt7jK, PID: 32560695
Zhang, S., Knockout of SlMAPK3 reduced disease resistance to Botrytis cinerea in tomato plants (2018) J Agric Food Chem, 66 (34), pp. 8949-8956. , COI: 1:CAS:528:DC%2BC1cXhsVOlsLfM, PID: 30092129
Prihatna, C., Barbetti, M.J., Barker, S.J., A novel tomato fusarium wilt tolerance gene (2018) Front Microbiol, 9, p. 1226. , PID: 29937759
Wang, F., Enhanced rice blast resistance by CRISPR/Cas9-targeted mutagenesis of the ERF transcription factor gene OsERF922 (2016) PLoS One, 11 (4). , PID: 27116122, COI: 1:CAS:528:DC%2BC28XhsVSgtL%2FE
Wang, X., CRISPR/Cas9-mediated efficient targeted mutagenesis in grape in the first generation (2017) Plant Biotechnol J, 16 (4), pp. 844-855
Nekrasov, V., Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion (2017) Sci Rep, 7 (1), p. 482. , PID: 28352080, COI: 1:CAS:528:DC%2BC1cXhs1yksbnM
Dale, J., Transgenic Cavendish bananas with resistance to Fusarium wilt tropical race 4 (2017) Nat Commun, 8 (1), p. 1496. , PID: 29133817, COI: 1:CAS:528:DC%2BC1cXovFygtro%3D
Xie, K., Yang, Y., RNA-guided genome editing in plants using a CRISPR–Cas system (2013) Mol Plant, 6 (6), pp. 1975-1983. , COI: 1:CAS:528:DC%2BC3sXhvVart7vP, PID: 23956122
Malnoy, M., DNA-free genetically edited grapevine and apple protoplast using CRISPR/Cas9 ribonucleoproteins (2016) Front Plant Sci, 7 (1904), p. 1904. , PID: 28066464
Wang, Y., Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew (2014) Nat Biotechnol, 32 (9), pp. 947-951. , COI: 1:CAS:528:DC%2BC2cXhtFygs7jJ, PID: 25038773
Zhang, Y., Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat (2017) Plant J, 91 (4), pp. 714-724. , COI: 1:CAS:528:DC%2BC2sXpslWjtLY%3D, PID: 28502081
Zhou, J., Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice (2015) Plant J, 82 (4), pp. 632-643. , COI: 1:CAS:528:DC%2BC2MXotVWntbk%3D, PID: 25824104
Kim, Y.A., Moon, H., Park, C.J., CRISPR/Cas9-targeted mutagenesis of Os8N3 in rice to confer resistance to Xanthomonas oryzae pv. oryzae (2019) Rice (N Y), 12 (1), p. 67
Jiang, W., Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice (2013) Nucleic Acids Res, 41 (20). , COI: 1:CAS:528:DC%2BC3sXhslWrt73E, PID: 23999092
Ortigosa, A., Design of a bacterial speck resistant tomato by CRISPR/Cas9-mediated editing of SlJAZ2 (2019) Plant Biotechnol J, 17 (3), pp. 665-673. , COI: 1:CAS:528:DC%2BC1MXjtlChsbo%3D, PID: 30183125
de Toledo Thomazella, D.P., (2016) CRISPR-Cas9 mediated mutagenesis of a DMR6 ortholog in tomato confers broad-spectrum disease resistance, p. p. doi. , https://doi.org/10.1101/064824
Jia, H., Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker (2017) Plant Biotechnol J, 15 (7), pp. 817-823. , COI: 1:CAS:528:DC%2BC2sXhtVWjsrfE, PID: 27936512
Peng, A., Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus (2017) Plant Biotechnol J, 15 (12), pp. 1509-1519. , COI: 1:CAS:528:DC%2BC2sXhvV2nsLrE, PID: 28371200
Gumtow, R., A Phytophthora palmivora extracellular cystatin-like protease inhibitor targets papain to contribute to virulence on papaya (2018) Mol Plant-Microbe Interact, 31 (3), pp. 363-373. , COI: 1:CAS:528:DC%2BC1cXhvFCqtrfK, PID: 29068239
Fister, A.S., Transient expression of CRISPR/Cas9 machinery targeting TcNPR3 enhances defense response in Theobroma cacao (2018) Front Plant Sci, 9, p. 268. , PID: 29552023
Zaidi, S.S., Engineering plants for geminivirus resistance with CRISPR/Cas9 system (2016) Trends Plant Sci, 21 (4), pp. 279-281. , COI: 1:CAS:528:DC%2BC28XitlSgu7g%3D, PID: 26880316
Mahas, A., Aman, R., Mahfouz, M., CRISPR-Cas13d mediates robust RNA virus interference in plants (2019) Genome Biol, 20 (1), p. 263. , COI: 1:CAS:528:DC%2BC1MXitlynsbnO, PID: 31791381
Hadidi, A., Next-generation sequencing and CRISPR/Cas13 editing in viroid research and molecular diagnostics (2019) Viruses, 11 (2), p. 120
Rybicki, E.P., CRISPR-Cas9 strikes out in cassava (2019) Nat Biotechnol, 37 (7), pp. 727-728. , COI: 1:CAS:528:DC%2BC1MXhtlSmsL7L, PID: 31197230
Zaidi, S.S., Engineering plant immunity: using CRISPR/Cas9 to generate virus resistance (2016) Front Plant Sci, 7, p. 1673. , PID: 27877187
Giraud, T., Gladieux, P., Gavrilets, S., Linking the emergence of fungal plant diseases with ecological speciation (2010) Trends Ecol Evol, 25 (7), pp. 387-395. , PID: 20434790
Acevedo-Garcia, J., Kusch, S., Panstruga, R., Magical mystery tour: MLO proteins in plant immunity and beyond (2014) New Phytol, 204 (2), pp. 273-281. , COI: 1:CAS:528:DC%2BC2cXhsFymtb%2FE, PID: 25453131
Freisleben, R., Lein, A., Über die Auffindung einer mehltauresistenten Mutante nach Röntgenbestrahlung einer anfälligen reinen Linie von Sommergerste (1942) Naturwissenschaften, 30 (40), p. 608
Jørgensen, I.H., Discovery, characterization and exploitation of Mlo powdery mildew resistance in barley (1992) Euphytica, 63 (1-2), pp. 141-152
van Schie, C.C., Takken, F.L., Susceptibility genes 101: how to be a good host (2014) Annu Rev Phytopathol, 52, pp. 551-581. , PID: 25001453, COI: 1:CAS:528:DC%2BC2cXhsl2ms7%2FJ
Maher, M.F., Plant gene editing through de novo induction of meristems (2020) Nat Biotechnol, 38 (1), pp. 84-89. , COI: 1:CAS:528:DC%2BC1MXisVSktb3L, PID: 31844292
Merker, L., Enhancing in planta gene targeting efficiencies in Arabidopsis using temperature‐tolerant CRISPR/LbCas12a (2020) Plant Biotechnol J, 18, pp. 2382-2384
Lu, Y., Tian, Y., Shen, R., Targeted, efficient sequence insertion and replacement in rice (2020) Nat Biotechnol, , https://doi.org/10.1038/s41587-020-0581-5
Barone, P., Efficient gene targeting in maize using inducible CRISPR-Cas9 and marker-free donor template (2020) Mol Plant, 13 (8), pp. 1219-1227. , COI: 1:CAS:528:DC%2BB3cXhsVClsL3K, PID: 32574856
Beying, N., CRISPR-Cas9-mediated induction of heritable chromosomal translocations in Arabidopsis (2020) Nat Plants, 6 (6), pp. 638-645. , COI: 1:CAS:528:DC%2BB3cXhtVelsr7L, PID: 32451449
Schmidt, C., Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering (2020) Nat Commun, 11 (1), p. 4418. , PID: 32887885
Hanley-Bowdoin, L., Geminiviruses: masters at redirecting and reprogramming plant processes (2013) Nat Rev Microbiol, 11 (11), pp. 777-788. , COI: 1:CAS:528:DC%2BC3sXhsFOitL3K, PID: 24100361
Yang, X., Geminivirus-associated betasatellites: exploiting chinks in the antiviral arsenal of plants (2019) Trends Plant Sci, 24 (6), pp. 519-529. , COI: 1:CAS:528:DC%2BC1MXms1Gqsr8%3D, PID: 31003895
Zubair, M., An insight into Cotton leaf curl Multan betasatellite, the most important component of cotton leaf curl disease complex (2017) Viruses, 9 (10), p. 280. , COI: 1:CAS:528:DC%2BC1cXitFeltr%2FI
Aguilar, E., Garnelo Gomez, B., Lozano-Duran, R., Recent advances on the plant manipulation by geminiviruses (2020) Curr Opin Plant Biol, 56, pp. 56-64. , COI: 1:CAS:528:DC%2BB3cXnsleltb8%3D, PID: 32464465
Custers, R., The regulatory status of gene-edited agricultural products in the EU and beyond (2017) Emerg Top Life Sci, 1 (2), pp. 221-229
Globus, R., Qimron, U., A technological and regulatory outlook on CRISPR crop editing (2017) J Cell Biochem, 119, pp. 1291-1298
Huang, S., A proposed regulatory framework for genome-edited crops (2016) Nat Genet, 48 (2), pp. 109-111. , COI: 1:CAS:528:DC%2BC28XhsFyitL8%3D, PID: 26813761
Eriksson, D., Why the European Union needs a national GMO opt-in mechanism (2018) Nat Biotechnol, 36 (1), pp. 18-19. , COI: 1:CAS:528:DC%2BC1cXmtlSkuw%3D%3D, PID: 29319683
Ali, Z., Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice (2020) Commun Biol, 3