Article (Scientific journals)
Nosocomial infections caused by multidrug-resistant isolates of Pseudomonas putida producing VIM-1 metallo-β-lactamase
Lombardi, G.; Luzzaro, F.; Docquier, Jean-Denis et al.
2002In Journal of Clinical Microbiology, 40 (11), p. 4051-4055
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Keywords :
Achromobacter; Escherichia coli; Gram negative infection; Italy; Pseudomonas aeruginosa; Pseudomonas putida; Aged; Carbapenems; Cross Infection; Drug Resistance, Multiple, Bacterial; Humans; Integrons; Microbial Sensitivity Tests; Middle Aged; Molecular Sequence Data; Pseudomonas Infections; Urinary Tract Infections; Achromobacter xylosoxidans; Bacteria (microorganisms); Negibacteria; Pseudomonas
Abstract :
[en] Successful carbapenem-based chemotherapy for the treatment of Pseudomonas infections has been seriously hindered by the recent appearance of IMP- and VIM-type metallo-β-lactamases, which confer high-level resistance to carbapenems and most other β-lactams. Recently, multidrug-resistant Pseudomonas putida isolates for which carbapenem MICs were ≥32 μg/ml were recovered from cultures of urine from three inpatients in the general intensive care unit of the Ospedale di Circolo, Varese, Italy. Enzyme assays revealed production of a metallo-β-lactamase activity, while molecular analysis detected in each isolate a blaVIM-1 determinant carried by an apparently identical medium-sized plasmid. Conjugation experiments were unsuccessful in transferring the β-lactamase determinant to Escherichia coli or Pseudomonas aeruginosa. Macrorestriction analysis by pulsed-field gel electrophoresis demonstrated that the isolates were of clonal origin. PCR mapping and sequencing of the variable region of the plasmid-borne class 1 integron carrying the blaVIM-1 determinant (named In110) showed that the blaVIM-1-containing cassette was identical to that previously found in strains of different species from other Italian hospitals and that the cassette array of In110 was not identical but clearly related to that of In70 (a blaVIM-1-containing plasmid-borne integron from an Achromobacter xylosoxidans isolate), pointing to a common origin of this cassette and to a related evolutionary history of their cognate integrons.
Disciplines :
Microbiology
Author, co-author :
Lombardi, G.;  Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 Siena, Italy
Luzzaro, F.;  Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 Siena, Italy
Docquier, Jean-Denis ;  Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
Riccio, M. L.;  Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 Siena, Italy
Perilli, M.;  Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 Siena, Italy
Colì, A.;  Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 Siena, Italy
Amicosante, G.;  Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 Siena, Italy
Rossolini, G. M.;  Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 Siena, Italy
Toniolo, A.;  Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 Siena, Italy
Language :
English
Title :
Nosocomial infections caused by multidrug-resistant isolates of Pseudomonas putida producing VIM-1 metallo-β-lactamase
Publication date :
2002
Journal title :
Journal of Clinical Microbiology
ISSN :
0095-1137
eISSN :
1098-660X
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
Volume :
40
Issue :
11
Pages :
4051-4055
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
European Training and Mobility of Researchers Network on Metallo-beta-Lactaqmases (grant no. FMRX-CT98-0232)
Available on ORBi :
since 24 November 2020

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