[en] A microdilution test measuring imipenem MICs in the presence or absence of a mixture of EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18 VIM-and 4 IMP-type metallo-β-lactamase (MBL) producers. The chelator mixture reduced by fourfold or more the imipenem MICs for MBL producers, while a lower effect or no effect was usually observed with MBL nonproducers.
Disciplines :
Microbiology
Author, co-author :
Migliavacca, R.; Dipartimento di Scienze Morfologiche, Sezione di Microbiologia, Universita di Pavia, Via Brambilla, 74, 27100 Pavia, Italy
Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines
Mugnaioli, C.; Dipartimento di Scienze Morfologiche, Sezione di Microbiologia, Universita di Pavia, Via Brambilla, 74, 27100 Pavia, Italy
Amicosante, G.; Dipartimento di Scienze Morfologiche, Sezione di Microbiologia, Universita di Pavia, Via Brambilla, 74, 27100 Pavia, Italy
Daturi, R.; Dipartimento di Scienze Morfologiche, Sezione di Microbiologia, Universita di Pavia, Via Brambilla, 74, 27100 Pavia, Italy
Lee, K.; Dipartimento di Scienze Morfologiche, Sezione di Microbiologia, Universita di Pavia, Via Brambilla, 74, 27100 Pavia, Italy
Rossolini, G. M.; Dipartimento di Scienze Morfologiche, Sezione di Microbiologia, Universita di Pavia, Via Brambilla, 74, 27100 Pavia, Italy
Pagani, L.; Dipartimento di Scienze Morfologiche, Sezione di Microbiologia, Universita di Pavia, Via Brambilla, 74, 27100 Pavia, Italy
Language :
English
Title :
Simple microdilution test for detection of metallo-β-lactamase production in pseudomonas aeruginosa
Publication date :
2002
Journal title :
Journal of Clinical Microbiology
ISSN :
0095-1137
eISSN :
1098-660X
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
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