Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa

[en] The prevalence and the type(s) of metallo-β-lactamases (MBLs) produced by isolates of Pseudomonas aeruginosa were investigated. During 2001, 506 nonduplicate isolates were obtained from hospitalized patients. Eighty-two strains were selected because of resistance to carbapenems and/or ceftazidime. Screening for MBL production was performed in the latter isolates by the Etest MBL strips (AB Biodisk, Solna, Sweden) and by a broth microdilution method measuring minimum inhibitory concentrations (MICs) of imipenem alone and in the presence of metal-chelating agents (EDTA and o-phenanthroline). Specific DNA probes were used to investigate the presence of genes coding for IMP- or VIM-type enzymes. Overall, four isolates of P. aeruginosa (obtained from independent patients) were found to carry a blaVIM gene. Polymerase chain reaction (PCR) experiments and DNA sequencing revealed that the VIM-2 determinant was present in three cases, whereas VIM-1 was detected in one isolate. Surveillance programs should be adopted to avoid the spread of these worrisome resistance genes. © 2004 Elsevier Inc. All rights reserved.

Disciplines :

Microbiology

Author, co-author :

Luzzaro, F.; Laboratorio di Microbiologia, Osp. Circolo and Univ. dell'Insubria, I-21100 Varese, Italy

Endimiani, A.; Laboratorio di Microbiologia, Osp. Circolo and Univ. dell'Insubria, I-21100 Varese, Italy

Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Mugnaioli, C.; Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, I-53100 Siena, Italy

Bonsignori, M.; Laboratorio di Microbiologia, Osp. Circolo and Univ. dell'Insubria, I-21100 Varese, Italy

Amicosante, G.; Dipto. Sci. e Tecnologie Biomediche, Università di l'Aquila, I-67100 L'Aquila, Italy

Rossolini, G. M.; Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, I-53100 Siena, Italy

Toniolo, A.; Laboratorio di Microbiologia, Osp. Circolo and Univ. dell'Insubria, I-21100 Varese, Italy

Language :

English

Title :

Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa

Publication date :

2004

Journal title :

Diagnostic Microbiology and Infectious Disease

ISSN :

0732-8893

Publisher :

Elsevier Inc.

Volume :

Issue :

Pages :

131-135

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

European Training and Mobility of Researchers Network on Metallo-beta-Lactaqmases (grant no. FMRX-CT98-0232)

Available on ORBi :

since 24 November 2020

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