Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa

[en] The prevalence and the type(s) of metallo-β-lactamases (MBLs) produced by isolates of Pseudomonas aeruginosa were investigated. During 2001, 506 nonduplicate isolates were obtained from hospitalized patients. Eighty-two strains were selected because of resistance to carbapenems and/or ceftazidime. Screening for MBL production was performed in the latter isolates by the Etest MBL strips (AB Biodisk, Solna, Sweden) and by a broth microdilution method measuring minimum inhibitory concentrations (MICs) of imipenem alone and in the presence of metal-chelating agents (EDTA and o-phenanthroline). Specific DNA probes were used to investigate the presence of genes coding for IMP- or VIM-type enzymes. Overall, four isolates of P. aeruginosa (obtained from independent patients) were found to carry a blaVIM gene. Polymerase chain reaction (PCR) experiments and DNA sequencing revealed that the VIM-2 determinant was present in three cases, whereas VIM-1 was detected in one isolate. Surveillance programs should be adopted to avoid the spread of these worrisome resistance genes. © 2004 Elsevier Inc. All rights reserved.

Disciplines :

Microbiology

Author, co-author :

Luzzaro, F.; Laboratorio di Microbiologia, Osp. Circolo and Univ. dell'Insubria, I-21100 Varese, Italy

Endimiani, A.; Laboratorio di Microbiologia, Osp. Circolo and Univ. dell'Insubria, I-21100 Varese, Italy

Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Mugnaioli, C.; Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, I-53100 Siena, Italy

Bonsignori, M.; Laboratorio di Microbiologia, Osp. Circolo and Univ. dell'Insubria, I-21100 Varese, Italy

Amicosante, G.; Dipto. Sci. e Tecnologie Biomediche, Università di l'Aquila, I-67100 L'Aquila, Italy

Rossolini, G. M.; Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, I-53100 Siena, Italy

Toniolo, A.; Laboratorio di Microbiologia, Osp. Circolo and Univ. dell'Insubria, I-21100 Varese, Italy

Language :

English

Title :

Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa

Publication date :

2004

Journal title :

Diagnostic Microbiology and Infectious Disease

ISSN :

0732-8893

Publisher :

Elsevier Inc.

Volume :

Issue :

Pages :

131-135

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

European Training and Mobility of Researchers Network on Metallo-beta-Lactaqmases (grant no. FMRX-CT98-0232)

Available on ORBi :

since 24 November 2020

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Bibliography

Bush K., Jacoby G.A., Medeiros A.A. A functional classification scheme for β-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother. 39:1995;1211-1233.
Docquier J.-D., Luzzaro F., Amicosante G., Toniolo A., Rossolini G.M. Multidrug-resistant Pseudomonas aeruginosa producing PER-1 extended-spectrum serine-β-lactamase and VIM-2 metallo-β-lactamase. Emerg Infect Dis. 7:2001;910-911.
Docquier J.-D., Riccio M.L., Mugnaioli C., Luzzaro F., Endimiani A., Toniolo A., Amicosante G., Rossolini G.M. IMP-12, a new plasmid-encoded metallo-β-lactamase from a Pseudomonas putida clinical isolate. Antimicrob Agents Chemother. 47:2003;1522-1528.
Kohler T., Michea-Hamzehpour T., Simone F.E., Pechere J.C. Carbapenem activities against Pseudomonas aeruginosa respective contribution of OprD and efflux systems . Antimicrob Agents Chemother. 43:1999;424-427.
Lauretti L., Riccio M.L., Mazzariol A., Cornaglia G., Amicosante G., Fontana R., Rossolini G.M. Cloning and characterization of blaVIM, a new integron-borne metallo-β-lactamase gene from a Pseudomonas aeruginosa clinical isolate. Antimicrob Agents Chemother. 43:1999;1584-1590.
Li X.-Z., Nikaido H., Poole K. Role of MexA-MexB-OprM in antibiotic efflux system in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 39:1995;1948-1953.
Lombardi G., Luzzaro F., Docquier J.-D., Riccio M.L., Perilli M., Colì A., Amicosante G., Rossolini G.M., Toniolo A. Nosocomial infections caused by multidrug-resistant isolates of Pseudomonas putida producing the VIM-1 metallo-β-lactamase. J Clin Microbiol. 40:2002;4051-4055.
Migliavacca R., Docquier J.-D., Mugnaioli C., Amicosante G., Daturi R., Lee K., Rossolini G.M., Pagani L. Simple microdilution test for detection of metallo-β-lactamase production in Pseudomonas aeruginosa. J Clin Microbiol. 40:2002;4388-4390.
Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 2000;NCCLS, Wayne, PA. Approved standard M7-A5.
Nordmann P., Poirel L. Emerging carbapenemases in Gram-negative aerobes. Clin Microbiol Infect. 8:2002;321-331.
Pai H., Kim J.-W., Kim J., Lee J.H., Choe K.W., Gotoh N. Carbapenem resistance mechanisms in Pseudomonas aeruginosa clinical isolates. Antimicrob Agents Chemother. 45:2001;480-484.
Poirel L., Naas T., Nicolas D., Collet L., Bellais S., Cavallo J.-D., Nordmann P. Characterization of VIM-2 a carbapenem-hydrolyzing metallo-β-lactamase and its plasmid- and integron-borne gene from a Pseudomonas aeruginosa clinical isolate in France. Antimicrob Agents Chemother. 44:2000;891-897.
Rossolini G.M., Riccio M.L., Cornaglia G., Pagani L., Lagatolla C., Selan L., Fontana R. Carbapenem-resistant Pseudomonas aeruginosa with acquired blaVIM metallo-β-lactamase determinants, Italy. Emerg Infect Dis. 6:2000;312-313.
Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning a laboratory manual . 3rd ed:2001;Cold Spring Harbor Laboratory Press, New York.
Senda K., Arakawa Y., Nakashima K., Ito H., Ichiyama S., Shimokata K., Kato N., Ohta M. Multifocal outbreaks of metallo-β-lactamase-producing Pseudomonas aeruginosa resistant to broad-spectrum β-lactams, including carbapenems. Antimicrob Agents Chemother. 40:1996;349-353.
Walsh T.R., Bolmström A., Qwärnström A., Gales A. Evaluation of a new Etest for detecting metallo-β-lactamases in routine clinical testing. J Clin Microbiol. 40:2002;2755-2759.
Yan J.-J., Hsueh P.-R., Ko W.-C., Luh K.-T., Tsai S.-H., Wu H.-M., Wu J.-J. Metallo-β-lactamases in clinical Pseudomonas isolates in Taiwan and identification of VIM-3, a novel variant of the VIM-2 enzyme. Antimicrob Agents Chemother. 45:2001;2224-2228.