Novel acquired metallo-β-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea

Lee, K.; Yum, J. H.; Yong, D.; Lee, H. M.; Kim, H. D.; Docquier, Jean-Denis; Rossolini, G. M.; Chong, Y.

doi:10.1128/AAC.49.11.4485-4491.2005

Article (Scientific journals)

Novel acquired metallo-β-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea

Lee, K.; Yum, J. H.; Yong, D. et al.

2005 • In Antimicrobial Agents and Chemotherapy, 49 (11), p. 4485-4491

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https://hdl.handle.net/2268/252993

DOI
10.1128/AAC.49.11.4485-4491.2005

PubMed
16251286

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Keywords :

Acinetobacter baumannii; Korea; MBL gene; Pseudomonas aeruginosa; Adolescent; Aged; Amino Acid Sequence; Base Sequence; Carbapenems; Child; Child, Preschool; Drug Resistance, Bacterial; Female; Humans; Integrons; Male; Microbial Sensitivity Tests; Middle Aged; Molecular Sequence Data

Abstract :

[en] Carbapenem resistance mediated by acquired carbapenemase genes has been increasingly reported, particularly for clinical isolates of Pseudomonas aeruginosa and Acinetobacter spp. Of 1,234 nonduplicate isolates of carbapenem-resistant Pseudomonas spp. and Acinetobacter spp. isolated at a tertiary-care hospital in Seoul, Korea, 211 (17%) were positive for metallo-β-lactamase (MBL). Of these, 204 (96%) had either the bla IMP-1 or blaVIM-2 allele. In addition, seven Acinetobacter baumannii isolates were found to have a novel MBL gene, which was designated blaSIM-1. The SIM-1 protein has a pI of 7.2, is a new member of subclass B1, and exhibits 64 to 69% identity with the IMF-type MBLs, which are its closest relatives. All SIM-1-producing isolates exhibited relatively low imipenem and meropenem MICs (8 to 16 μg/ml) and had a multidrug resistance phenotype. Expression of the cloned blaSIM-1 gene in Escherichia coli revealed that the encoded enzyme is capable of hydrolyzing a broad array of β-lactams, including penicillins, narrow- to expanded-spectrum cephalosporins, and carbapenems. The blaSIM-1 gene was carried on a gene cassette inserted into a class 1 integron, which included three additional cassettes (arr-3, catB3, and aadA1). The strains were isolated from sputum and urine specimens from patients with pneumonia and urinary tract infections, respectively. All patients had various underlying diseases. Pulsed-field gel electrophoresis of SmaI-digested genomic DNAs showed that the strains belonged to two different clonal lineages, indicating that horizontal transfer of this gene had occurred and suggesting the possibility of further spread of resistance in the future. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Disciplines :

Microbiology

Author, co-author :

Lee, K.; Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul 120-752, South Korea, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-752, South Korea, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, South Korea

Yum, J. H.; Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-752, South Korea, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, South Korea

Yong, D.; Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul 120-752, South Korea, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-752, South Korea, Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, South Korea

Lee, H. M.; Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul 120-752, South Korea, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-752, South Korea

Kim, H. D.; Department of Pediatrics, Yonsei University College of Medicine, Seoul 120-752, South Korea

Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Rossolini, G. M.; Department of Molecular Biology, Microbiology Section, University of Siena, Siena I-53100, Italy

Chong, Y.; Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul 120-752, South Korea, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 120-752, South Korea, Department of Laboratory Medicine, Yonsei University College of Medicine, 134 Shinchondong, Seodaemunku, Seoul 120-752, South Korea

Language :

English

Title :

Novel acquired metallo-β-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea

Publication date :

2005

Journal title :

Antimicrobial Agents and Chemotherapy

ISSN :

0066-4804

eISSN :

1098-6596

Publisher :

American Society for Microbiology, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

4485-4491

Peer reviewed :

Peer Reviewed verified by ORBi

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