Article (Scientific journals)
bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting MDFIC Gene
Hu, Xin; Xing, Yishen; Ren, Ling et al.
2020In Genes, 20, p. 1232
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Keywords :
bta-miR-23a; fetal muscle development; MDFIC
Abstract :
[en] miR-23a, a member of the miR-23a/24-2/27a cluster, has been demonstrated to play pivotal roles in many cellular activities. However, the mechanisms of how bta-miR-23a controls the myogenic differentiation (MD) of PDGFRα − bovine progenitor cells (bPCs) remain poorly understood. In the present work, bta-miR-23a expression was increased during the MD of PDGFRα− bPCs. Moreover, bta-miR-23a overexpression significantly promoted the MD of PDGFRα− bPCs. Luciferase reporter assays showed that the 3’-UTR region of MDFIC (MyoD family inhibitor domain containing) could be a promising target of bta-miR-23a, which resulted in its post-transcriptional down-regulation. Additionally, the knockdown of MDFIC by siRNA facilitated the MD of PDGFRα− bPCs, while the overexpression of MDFIC inhibited the activating effect of bta-miR-23a during MD. Of note, MDFIC might function through the interaction between MyoG transcription factor and MEF2C promoter. This study reveals that bta-miR-23a can promote the MD of PDGFRα− bPCs through post-transcriptional downregulation of MDFIC.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Hu, Xin
Xing, Yishen
Ren, Ling
Wang, Yahui
Li, Qian
Yang, Qiyuan
Du, Min
Xu, Lingyang
Willems, Luc  ;  Université de Liège - ULiège > Cancer-Cellular and Molecular Epigenetics
Li, Junya
Zhang, Lupei
Language :
English
Title :
bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting MDFIC Gene
Publication date :
20 October 2020
Journal title :
Genes
ISSN :
2073-4425
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Switzerland
Volume :
20
Pages :
1232
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NSCF - National Natural Science Foundation of China [CN]
Cattle Breeding Innovative Research Team
Project of College Innovation Improvement under Beijing Municipality
Available on ORBi :
since 23 November 2020

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