Molecular heterogeneity of blaVIM-2-containing integrons from Pseudomonas aeruginosa plasmids encoding the VIM-2 metallo-β-lactamase

Pallecchi, L.; Riccio, M. L.; Docquier, Jean-Denis; Fontana, R.; Rossolini, G. M.

doi:10.1016/S0378-1097(01)00002-7

Article (Scientific journals)

Molecular heterogeneity of blaVIM-2-containing integrons from Pseudomonas aeruginosa plasmids encoding the VIM-2 metallo-β-lactamase

Pallecchi, L.; Riccio, M. L.; Docquier, Jean-Denis et al.

2001 • In FEMS Microbiology Letters, 195 (2), p. 145-150

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/252860

DOI
10.1016/S0378-1097(01)00002-7

PubMed
11179643

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Keywords :

Metallo-β-lactamase; Plasmid; Pseudomonas aeruginosa; Acetyltransferases; Amino Acid Sequence; Base Sequence; DNA Transposable Elements; Integrases; Molecular Sequence Data; Plasmids; Recombination, Genetic; Pseudomonas

Abstract :

[en] A blaVIM-2 metallo-β-lactamase determinant, identical to that previously identified in Pseudomonas aeruginosa COL-1 isolate from a French hospital, was detected on a 28-kb plasmid carried by a nosocomial isolate of P. aeruginosa from Verona, Italy. In this plasmid the blaVIM-2 determinant was inserted into a class 1 integron of original structure, named In72, that contains a partially deleted intI1 integrase gene and two gene cassettes. The first cassette carries an aacA4 aminoglycoside acetyl transferase determinant. The second cassette carries a blaVIM-2 determinant followed by a partially deleted attC site. The structure of In72 was notably different from that of In56, the blaVIM-2-containing integron found in the COL-1 isolate, revealing the existence of molecular heterogeneity among blaVIM-2-containing integrons in clinical isolates of P. aeruginosa from Europe. © 2001 Federation of European Microbiological Societies.

Disciplines :

Microbiology

Author, co-author :

Pallecchi, L.; Dipartimento Di Biologia Molecolare, Sezione Di Microbiologia, Università Di Siena, Via Laterina, 53100 Siena, Italy

Riccio, M. L.; Dipartimento Di Biologia Molecolare, Sezione Di Microbiologia, Università Di Siena, Via Laterina, 53100 Siena, Italy

Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Fontana, R.; Dipartimento Di Patologia, Sezione Di Microbiologia, Università Di Verona, 37134 Verona, Italy

Rossolini, G. M.; Dipartimento Di Biologia Molecolare, Sezione Di Microbiologia, Università Di Siena, Via Laterina, 53100 Siena, Italy

Language :

English

Title :

Molecular heterogeneity of blaVIM-2-containing integrons from Pseudomonas aeruginosa plasmids encoding the VIM-2 metallo-β-lactamase

Publication date :

2001

Journal title :

FEMS Microbiology Letters

ISSN :

0378-1097

eISSN :

1574-6968

Publisher :

Oxford University Press, United Kingdom

Volume :

195

Issue :

Pages :

145-150

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

European research network on metallo-L-lactamases within the Training and Mobility of Researchers program (Contract No. FMRXCT98-0232)

Funders :

UE - Union Européenne
MIUR - Ministero dell'Istruzione, dell'Università e della Ricerca

Available on ORBi :

since 20 November 2020

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