BEL-2, an extended-spectrum β-lactamase with increased activity toward expanded-spectrum cephalosporins in Pseudomonas aeruginosa

Poirel, L.; Docquier, Jean-Denis; De Luca, F.; Verlinde, A.; Ide, L.; Rossolini, G. M.; Nordmann, P.

doi:10.1128/AAC.00859-09

Article (Scientific journals)

BEL-2, an extended-spectrum β-lactamase with increased activity toward expanded-spectrum cephalosporins in Pseudomonas aeruginosa

Poirel, L.; Docquier, Jean-Denis; De Luca, F. et al.

2010 • In Antimicrobial Agents and Chemotherapy, 54 (1), p. 533-535

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/252856

DOI
10.1128/AAC.00859-09

PubMed
19884378

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Keywords :

Pseudomonas aeruginosa; Belgium; Cephalosporinase; Cephalosporins; Cross Infection; Escherichia coli; Genes, Bacterial; Humans; Kinetics; Microbial Sensitivity Tests; Pseudomonas Infections

Abstract :

[en] A Pseudomonas aeruginosa isolate recovered in Belgium produced a novel extended-spectrum ß-lactamase, BEL-2, differing from BEL-1 by a single Leu162Phe substitution. That modification significantly altered the kinetic properties of the enzyme, increasing its affinity for expanded-spectrum cephalosporins. The blaBEL-2 gene was identified from a P. aeruginosa isolate clonally related to another blaBEL-1-positive isolate. Copyright © 2010, American Society for Microbiology. All Rights Reserved.

Disciplines :

Biochemistry, biophysics & molecular biology
Microbiology

Author, co-author :

Poirel, L.; Service de Bactériologie-Virologie, Hôpital de Bicêtre, Université Paris-Sud, K.-Bicêtre, France

Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

De Luca, F.; Dipartimento di Biologia Molecolare, Università di Siena, Policlinico Santa Maria Alle Scotte, I-53100 Siena, Italy

Verlinde, A.; Department of Microbiology, Heilig Hartziekenhuis Roeselare, Wilgenstraat 2, 8800 Roeselare, Belgium

Ide, L.; Department of Microbiology, Heilig Hartziekenhuis Roeselare, Wilgenstraat 2, 8800 Roeselare, Belgium

Rossolini, G. M.; Dipartimento di Biologia Molecolare, Università di Siena, Policlinico Santa Maria Alle Scotte, I-53100 Siena, Italy

Nordmann, P.; Service de Bactériologie-Virologie, Hôpital de Bicêtre, Université Paris-Sud, K.-Bicêtre, France

Language :

English

Title :

BEL-2, an extended-spectrum β-lactamase with increased activity toward expanded-spectrum cephalosporins in Pseudomonas aeruginosa

Publication date :

2010

Journal title :

Antimicrobial Agents and Chemotherapy

ISSN :

0066-4804

eISSN :

1098-6596

Publisher :

American Society for Microbiology, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

533-535

Peer reviewed :

Peer Reviewed verified by ORBi

European Projects :

FP7 - 223031 - TROCAR - Translational Research on Combating Antimicrobial Resistance

Funders :

UE - Union Européenne
CE - Commission Européenne

Available on ORBi :

since 20 November 2020

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