Characteristics of clinical isolates of Acinetobacter genomospecies 10 carrying two different metallo-β-lactamases

Acinetobacter genomospecies 10; Imipenem resistance; MBL genes; Acinetobacter; Acinetobacter infection; DNA sequence; Acinetobacter Infections; Adult; Aged; Anti-Bacterial Agents; Bacterial Proteins; DNA, Bacterial; Female; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Molecular Sequence Data; Sequence Analysis, DNA

Abstract :

[en] Acquired metallo-β-lactamase (MBL) production is an important mechanism of carbapenem resistance. To our knowledge, carriage of two different MBLs has not been described previously in Acinetobacter spp. We present the characteristics of five Acinetobacter isolates carrying two different MBL genes. The species of all five Acinetobacter isolates with two different MBL genes were Acinetobacter genomospecies 10, and blaIMP-1, blaVIM-2 and blaSIM-1 may coexist in this species. Minimum inhibitory concentrations (MICs) of imipenem for all five isolates with two different MBLs were ≥32μg/mL, whilst those for two segregants that lost both MBLs were 0.5μg/mL. The presence of MBL gene-carrying integrons with identical structures suggested the easily transferable nature of the elements, whilst instability of the MBL genes indicated potentially erroneous phenotypic and genetic characterisation. © 2010 Elsevier B.V. and the International Society of Chemotherapy.

Disciplines :

Microbiology

Author, co-author :

Lee, K.; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea

Kim, C. K.; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea

Hong, S. G.; Department of Laboratory Medicine, College of Medicine, Cha University, Seongnam, South Korea

Choi, J.; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea

Song, S.; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea

Koh, E.; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea

Yong, D.; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea

Jeong, S. H.; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea

Yum, J. H.; Department of Clinical Laboratory Science, Dong-eui University, Busan, South Korea

Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Rossolini, G. M.; Department of Molecular Biology, Section of Microbiology, University of Siena, Siena, Italy

Chong, Y.; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, South Korea

Language :

English

Title :

Characteristics of clinical isolates of Acinetobacter genomospecies 10 carrying two different metallo-β-lactamases

Publication date :

2010

Journal title :

International Journal of Antimicrobial Agents

ISSN :

0924-8579

eISSN :

1872-7913

Publisher :

Elsevier, Amsterdam, Netherlands

Volume :

Issue :

Pages :

259-263

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 20 November 2020

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Bibliography

Bush K. New β-lactamases in Gram-negative bacteria: diversity and impact on the selection of antimicrobial therapy. Clin Infect Dis 2001, 32:1085-1089.
Lee M.-F., Peng C.-F., Hsu H.J., Chen Y.-H. Molecular characterisation of the metallo-β-lactamase genes in imipenem-resistant Gram-negative bacteria from a university hospital in southern Taiwan. Int J Antimicrob Agents 2008, 32:475-480.
Lee K., Yum J.H., Yong D., Lee H.M., Kim H.D., Docquier J.-D., et al. Novel acquired metallo-β-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea. Antimicrob Agents Chemother 2005, 49:4485-4491.
Lim Y.M., Shin K.S., Kim J. Distinct antimicrobial resistance patterns and antimicrobial resistance-harboring genes according to genomic species of Acinetobacter isolates. J Clin Microbiol 2007, 45:902-905.
Yan J.J., Ko W.C., Tsai S.H., Wu H.M., Wu J.J. Outbreak of infection with multidrug-resistant Klebsiella pneumoniae carrying blaIMP-8 in a university medical center in Taiwan. J Clin Microbiol 2001, 39:4433-4439.
Takahashi A., Yomoda S., Kobayashi I., Okubo T., Tsunoda M., Iyobe S. Detection of carbapenemase-producing Acinetobacter baumannii in a hospital. J Clin Microbiol 2000, 38:526-529.
Lee K., Yong D., Yum J.H., Lim Y.S., Bolmstrom A., Qwarnstrom A., et al. Evaluation of Etest MBL for detection of blaIMP-1 and blaVIM-2 allele-positive clinical isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol 2005, 43:942-944.
Lim Y.S., Yong D., Yum J.H., Lee K., Chong Y. Difficulties in the detection of resistance gene and the determination of imipenem resistance of the metallo-β-lactamase-producing Gram-negative bacilli. 42nd Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), 27-30 September 2002, San Diego, CA 2002, 139. ASM Press, Washington, DC, [Abstr. D 530].
Turton J.F., Kaufmann M.E., Coelho J.M., Warner M., Pike R., Pitt T.L. Detection and typing of integrons in epidemic strains of Acinetobacter baumannii found in the United Kingdom. J Clin Microbiol 2005, 43:3074-3082.
Lee K., Kim M.-N., Choi T.Y., Cho S.-E., Lee S., Whang D.H., et al. Wide dissemination of OXA-type carbapenemases in clinical Acinetobacter spp. isolates from South Korea. Int J Antimicrob Agents 2009, 33:520-524.
Chang H.C., Wei Y.F., Dijkshoorn L., Vaneechoutte M., Tang C.T., Chang T.C. Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence analysis of the 16S-23S rRNA gene spacer region. J Clin Microbiol 2005, 43:1632-1639.
Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Nineteenth informational supplement. Document M100-S19. Wayne, PA: CLSI; 2009.
Tsakris A., Ikonomidis A., Poulou A., Spanakis N., Vrizas D., Diomidous M., et al. Clusters of imipenem-resistant Acinetobacter baumannii clones producing different carbapenemases in an intensive care unit. Clin Microbiol Infect 2008, 14:588-594.
Gombac F., Riccio M.L., Rossolini G.M., Lagatolla C., Tonin E., Monti-Bragadin C., et al. Molecular characterization of integrons in epidemiologically unrelated clinical isolates of Acinetobacter baumannii from Italian hospitals reveals a limited diversity of gene cassette arrays. Antimicrob Agents Chemother 2002, 46:3665-3668.
Barton B.M., Harding G.P., Zuccarelli A.J. A general method for detecting and sizing large plasmids. Anal Biochem 1995, 226:235-240.