Regulation of neuraminidase expression in Streptococcus pneumoniae

Carbon catabolite repression; Metabolic regulation; Sialic acid; ABC transporter; Streptococcus; Streptococcus gordonii; Streptococcus mitis; Streptococcus oralis; Streptococcus pneumoniae; Streptococcus sanguinis; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Hexosamines; Humans; N-Acetylneuraminic Acid; Neuraminidase; Operon

Abstract :

[en] Background: Sialic acid (N-acetylneuraminic acid; NeuNAc) is one of the most important carbohydrates for Streptococcus pneumoniae due of its role as a carbon and energy source, receptor for adhesion and invasion and molecular signal for promotion of biofilm formation, nasopharyngeal carriage and invasion of the lung. Results: In this work, NeuNAc and its metabolic derivative N-acetyl mannosamine (ManNAc) were used to analyze regulatory mechanisms of the neuraminidase locus expression. Genomic and metabolic comparison to Streptococcus mitis, Streptococcus oralis, Streptococcus gordonii and Streptococcus sanguinis elucidates the metabolic association of the two amino sugars to different parts of the locus coding for the two main pneumococcal neuraminidases and confirms the substrate specificity of the respective ABC transporters. Quantitative gene expression analysis shows repression of the locus by glucose and induction of all predicted transcriptional units by ManNAc and NeuNAc, each inducing with higher efficiency the operon encoding for the transporter with higher specificity for the respective amino sugar. Cytofluorimetric analysis demonstrated enhanced surface exposure of NanA on pneumococci grown in NeuNAc and ManNAc and an activity assay allowed to quantify approximately twelve times as much neuraminidase activity on induced cells as opposed to glucose grown cells. Conclusions: The present data increase the understanding of metabolic regulation of the nanAB locus and indicate that experiments aimed at the elucidation of the relevance of neuraminidases in pneumococcal virulence should possibly not be carried out on bacteria grown in glucose containing media. © 2012 Gualdi et al.; licensee BioMed Central Ltd.

Disciplines :

Microbiology

Author, co-author :

Gualdi, L.; Dipartimento di Biotecnologie, Universit di Siena, Siena, Italy

Hayre, J. K.; Dipartimento di Biotecnologie, Universit di Siena, Siena, Italy

Gerlini, A.; Dipartimento di Biotecnologie, Universit di Siena, Siena, Italy

Bidossi, A.; Dipartimento di Biotecnologie, Universit di Siena, Siena, Italy

Colomba, L.; Dipartimento di Biotecnologie, Universit di Siena, Siena, Italy

Trappetti, C.; Dipartimento di Biotecnologie, Universit di Siena, Siena, Italy, Research Centre for Infectious Diseases, School of Molecular and Biomedical Science, University of Adelaide, SA 5005, Australia

Pozzi, G.; Dipartimento di Biotecnologie, Universit di Siena, Siena, Italy

Docquier, Jean-Denis ; Université de Liège - ULiège > Département des sciences de la vie > Centre d'ingénierie des protéines

Andrew, P.; Department of Infection Immunity and Inflammation, University of Leicester, Leicester, United Kingdom

Ricci, S.; Dipartimento di Biotecnologie, Universit di Siena, Siena, Italy

Oggioni, M. R.; Dipartimento di Biotecnologie, Universit di Siena, Siena, Italy, UOC Batteriologia, Azienda Universitaria Ospedaliera Senese, Siena, Italy, LAMMB, Policlinico le Scotte Lotto, 5 piano 1, Siena, 53100, Italy, Research Centre for Infectious Diseases, School of Molecular and Biomedical Science, University of Adelaide, SA 5005, Australia

Language :

English

Title :

Regulation of neuraminidase expression in Streptococcus pneumoniae

Publication date :

2012

Journal title :

BMC Microbiology

eISSN :

1471-2180

Publisher :

BioMed Central, United Kingdom

Volume :

Peer reviewed :

Peer Reviewed verified by ORBi

European Projects :

FP7 - 222983 - PNEUMOPATH - A comprehensive dissection of pneumococcal-host interactions

Funders :

CE - Commission Européenne

Available on ORBi :

since 19 November 2020

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